Avaliação do uso tópico do metronidazol no processo de cicatrização de feridas: um estudo experimental

Trindade, Lilian Cristine Teixeira; Biondo-Simões, Maria de Lourdes Pessole; Sampaio, Cláudia Paraguaçu Pupo; Farias, Rogério Estevam; Pierin, Rodrigo Jardim; Netto, Miguel Chomiski

doi:10.1590/s0100-69912010000500009

Cited by 15 publications

(11 citation statements)

References 15 publications

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“…It has been used systemically and/or topically to treat wound infections, for example, fungating carcinomas and necrotic pressure ulcers [45,46], because of its ability to reduce the malodor of anaerobically colonized wounds (malodorous wounds). Moreover, two studies suggested that topical metronidazole increased epithelialization during wound healing by secondary intention in rats [47,48]. However, most treatments use conventional gel preparations for MZ, which require generous application and need a secondary dressing.…”

Section: Introductionmentioning

confidence: 99%

Thermo-Responsive Poly(N-Isopropylacrylamide)-Cellulose Nanocrystals Hybrid Hydrogels for Wound Dressing

Zubik¹,

Singhsa

Wang³

et al. 2017

Polymers

137

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Thermo-responsive hydrogels containing poly(N-isopropylacrylamide) (PNIPAAm), reinforced both with covalent and non-covalent interactions with cellulose nanocrystals (CNC), were synthesized via free-radical polymerization in the absence of any additional cross-linkers. The properties of PNIPAAm-CNC hybrid hydrogels were dependent on the amounts of incorporated CNC. The thermal stability of the hydrogels decreased with increasing CNC content. The rheological measurement indicated that the elastic and viscous moduli of hydrogels increased with the higher amounts of CNC addition, representing stronger mechanical properties of the hydrogels. Moreover, the hydrogel injection also supported the hypothesis that CNC reinforced the hydrogels; the increased CNC content exhibited higher structural integrity upon injection. The PNIPAAm-CNC hybrid hydrogels exhibited clear thermo-responsive behavior; the volume phase transition temperature (VPTT) was in the range of 36 to 39 • C, which is close to normal human body temperature. For wound dressing purposes, metronidazole, an antibiotic and antiprotozoal often used for skin infections, was used as a target drug to study drug-loading and the release properties of the hydrogels. The hydrogels showed a good drug-loading capacity at room temperature and a burst drug release, which was followed by slow and sustained release at 37 • C. These results suggested that newly developed drugs containing injectable hydrogels are promising materials for wound dressing.

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Section: Introductionmentioning

confidence: 99%

Thermo-Responsive Poly(N-Isopropylacrylamide)-Cellulose Nanocrystals Hybrid Hydrogels for Wound Dressing

Zubik¹,

Singhsa

Wang³

et al. 2017

Polymers

137

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“…In a study that evaluated the chondrocyte proliferation activity by the expression of cellular proliferation nuclear antigen in the zone of cartilage growth after 30 days of subtotal 5/6 nephrectomy in rats, it was possible to conclude that the number of proliferating cells in the growth plateau was significantly lower in uremic rats when compared to control rats 1 . Another experimental study in rats that investigated the density of myofibroblasts in wound healing by the same method employed in this study concluded that metronidazole, applied topically to wound healing with second intention, does not interfere with the contraction of the wound and delays the appearance of myofibroblasts 32 .…”

Section: Discussionmentioning

confidence: 78%

Effects of Renal Dysfunction on Healing of Colonic Anastomosis: Experimental Study in Wistar Rats

Silva

Repka

Souza³

et al. 2018

ABCD, arq. bras. cir. dig.

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Background: Chronic kidney disease affects more than 500 million people worldwide. In this context, the uremic toxins present are related to worsening in tissue healing. Aim: Evaluate on healing of colonic anastomosis in uremic rats, serum and anatomopathological indicators, which may be related to the change tissue repair process. Methods: Twenty Wistar rats, were randomly separated into two groups. In the sham group they were submitted to 5/6 nephrectomy simulation in left kidney, simulation right nephrectomy, median laparotomy, colotomy and colorraphy. In the uremia group, they were submitted to 5/6 nephrectomy of the left kidney, total nephrectomy of the right kidney and median laparotomy, colotomy and colorraphy. Were collected for serum urea, creatinine and CRP dosages and the colonic segments were studied for evaluation of granulation tissue, collagen maturation, microvascular and myofibroblasts density, and cell viability. Through histochemical processing, microvascular density was evaluated by anti-CD34 monoclonal antibody marking, cell viability by cell proliferation nuclear antigen screening and myofibroblasts density with monoclonal anti-α-actin antibody. Computerized histometry was used for evaluations of collagens type I and III by the coloration of picrosirius. Results: The group submitted to nephrectomy 5/6, compared to the sham group, show urea increase (p<0.0000) and higher C reactive protein (p=0.0142). Decrease of granulation tissue formation (border reepithelialization p=0,0196, angiofibroblast proliferation p=0.0379), mean collagen I (p=0,0009) and collagen III (p=0,016), microvascular density (p=0,0074), cell proliferation nuclear antigen (p<0,0000) and myofibroblasts (p<0,0001). Conclusion: The uremia induced by nephrectomy 5/6 model establishes negative impact in the colonic wound healing.

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“…In the present study, the tissue densities of myofibroblasts did not differ between the arginine and control groups (p=0.0511), although there was a numerical difference. An experimental study in rats that investigated the density of myofibroblasts in wound healing by the same method employed here concluded that metronidazole, applied topically to wounds with secondary intention healing, does not interfere with wound contraction and delays the appearance of myofibroblasts 23 .…”

Section: Figure 2 Comparison Between the Mean Percentages Of Collagementioning

confidence: 78%

Efeitos da arginina na reparação cicatricial da parede abdominal em ratos Wistar.

Caron

Repka

Milgioransa

et al. 2019

Rev. Col. Bras. Cir.

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RESUMO Objetivo: avaliar os efeitos da arginina na cicatrização da parede abdominal de ratos Wistar. Métodos: vinte ratos Wistar foram submetidos à laparotomia e separados em dois grupos (arginina e controle), que receberam tratamento diário por via intraperitoneal com arginina (300mg/kg/dia) e solução tampão fosfato em dose equivalente ao peso, respectivamente, durante cinco dias. No sétimo dia pós-operatório, coletaram-se amostras de sangue e da cicatriz da parede abdominal de ambos os grupos. Avaliaram-se o nível sérico de nitratos e nitritos, a evolução cicatricial pelas dosagens de hidroxiprolina tecidual, formação de tecido de granulação, determinação da porcentagem de colágeno maduro e imaturo, densidade de miofibroblastos e angiogênese. Empregaram-se os testes de ANOVA e t de Student com p=0,05 para as comparações entre os grupos. Resultados: não ocorreram diferenças significantes entre os grupos estudados para dosagens de nitratos e nitritos (p=0,9903), hidroxiprolina tecidual (p=0,1315) e densidade de miofibroblastos (p=0,0511). O grupo arginina apresentou maior densidade microvascular (p=0,0008), maior porcentagem de colágeno tipo I (p=0,0064) e melhora na formação do tecido de granulação, com melhores índices de proliferação angiofibroblástica (p=0,0007) e re-epitelização das bordas (p=0,0074). Conclusão: na avaliação cicatricial da parede abdominal de ratos Wistar sob tratamento com arginina, não houve alteração do nível sérico de nitratos e nitritos, da deposição de colágeno total e da densidade de miofibroblastos. Verificaram-se aumento da maturação de colágeno do tipo I, da densidade microvascular e melhora na formação do tecido de granulação cicatricial pelas melhores re-epitelização de bordas e proliferação angiofibroblástica.

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Avaliação do uso tópico do metronidazol no processo de cicatrização de feridas: um estudo experimental

Abstract: Metronidazole 4% solution at a dose of 50 mg/kg applied topically to wounds healing by secondary intention facilitates early peripheral epithelialization, does not interfere with wound contraction and delays the appearance of myofibroblasts.

Cited by 15 publications

References 15 publications

Thermo-Responsive Poly(N-Isopropylacrylamide)-Cellulose Nanocrystals Hybrid Hydrogels for Wound Dressing

Thermo-Responsive Poly(N-Isopropylacrylamide)-Cellulose Nanocrystals Hybrid Hydrogels for Wound Dressing

Effects of Renal Dysfunction on Healing of Colonic Anastomosis: Experimental Study in Wistar Rats

Efeitos da arginina na reparação cicatricial da parede abdominal em ratos Wistar.

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