Desenvolvimento de modelo experimental de endometriose em ratas

Amaral, Vivian Ferreira do; Lago, Eduardo Andreazza Dal; Kondo, William; Guarita-Souza, Luiz César; Francisco, Júlio César

doi:10.1590/s0100-69912009000300012

Cited by 18 publications

(16 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They implanted total sections of uterus to abdominal wall of rats. In their allograft transplantation, they found non-signi cant differences in the total weight of animals [20]. In contrast, we found weight loss in allograft and xenograft transplantations to the abdominal wall of mice than transplantation to mesentery layer.…”

Section: Discussioncontrasting

confidence: 63%

Presentation of a New Endometriosis Mice Model Through Rat to Mice Xenograft Transplantation of Endometrium with some Modifications; A Pilot Study

Abdolmaleki¹,

Jalili²,

Mansouri³

et al. 2021

Preprint

View full text Add to dashboard Cite

Human endometriosis can lead to many complications, including permanent infertility. Rodent model of endometriosis is complicated due to the impractical isolation of myometrium. In this method, we defined a new model of endometriosis with some modifications. 40 female mice were grouped as; 1st sham, 2nd mice-mice allograft uterus transplantation of mice to anterior abdominal wall of mice, 3rd mice-mice allograft uterus transplantation of mice to mesentery of mice, 4th rat-mice xenograft endometrial transplantation of rat to anterior abdominal wall of mice, 5th rat-mice xenograft endometrial transplantation of rat to mesentery of mice. In recipient animals, estrous cycle was synchronized. Endometrium was dissected in rats and transplanted in abdominal wall or mesentery layer. Angiogenetic and inflammatory factors, serum levels of CA125, oxidative stress in peritoneal fluid, physical and histopathological features, and genes expression in uterus were assessed. Peritoneal concentrations of VEGF-A, TNF-α, NO, MDA, and serum levels of CA125, IL-37 were increased significantly (P < 0.05) in transplanted groups, especially in the 4th group than control. Total body weight of animals was decreased significantly (P < 0.05), while weight and size of endometrial lesions were increased significantly (P > 0.05). Genes expression of HOXA10 and HOXA11 were decreased in endometrium of uterus. Finally, histopathological features of endometrial lesions were confirmed by H&E and Perl staining. Xenograft transplantation of endometrium from rat to anterior abdominal wall of mice can potentially mimic the human endometriosis morphologically, histologically and genetically.

show abstract

Section: Discussioncontrasting

confidence: 63%

Presentation of a New Endometriosis Mice Model Through Rat to Mice Xenograft Transplantation of Endometrium with some Modifications; A Pilot Study

Abdolmaleki¹,

Jalili²,

Mansouri³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Two weeks post-surgery, 100% of animals implanted in the gastrocnemius muscle with uterus tissue developed a cystic lesion, which contrasts with the lower rate of success reported for the induction of peritoneal endometriosis-like lesions (Cason et al, 2003; Nogueira Neto et al, 2007; Do Amaral et al, 2009). Particular characteristics of skeletal muscle as a tissue receptor might have contributed to the high rate of cystic lesion induction observed here.…”

Section: Discussionmentioning

confidence: 69%

Ectopic uterine tissue as a chronic pain generator

et al. 2012

View full text Add to dashboard Cite

While chronic pain is a main symptom in endometriosis, the underlying mechanisms and effective therapy remain elusive. We developed an animal model enabling exploration of ectopic endometrium as a source of endometriosis pain. Rats were surgically implanted with autologous uterus in the gastrocnemius muscle. Within two weeks, visual inspection revealed the presence of a reddish-brown fluid filled cystic structure at the implant site. Histology demonstrated cystic glandular structures with stromal invasion of the muscle. Immunohistochemical studies of these lesions revealed the presence of markers for nociceptor nerve fibers and neuronal sprouting. Fourteen days after surgery rats exhibited persistent mechanical hyperalgesia at the site of the ectopic endometrial lesion. Intralesional, but not contralateral, injection of progesterone was dose-dependently antihyperalgesic. Systemic administration of leuprolide also produced antihyperalgesia. In vivo electrophysiological recordings from sensory neurons innervating the lesion revealed a significant increase in their response to sustained mechanical stimulation. These results are consistent with clinical and pathologic findings observed in patients with endometriosis, compatible with the ectopic endometrium as a source of pain. This model of endometriosis allows mechanistic exploration at the lesion site facilitating our understanding of endometriosis pain.

show abstract

“…Serosal side of the uterus was attached directly to the peritoneum as endometrial side was always facing the abdominal cavity. 22 The rats were individually caged after the operation and were left for a recovery period. After 4 weeks, their daily vaginal smears were monitored, and a second laparotomy was performed in their estrus phase to determine the attachment and viability of endometrial implants.…”

Section: Methodsmentioning

confidence: 99%