Expressão citofotométrica do fator de proliferação celular ki-67 no bócio colóide e no carcinoma papilífero da tireóide

Souza, Gleim Dias de; Czeczko, Nicolau Gregori; Moreira, Hamílton; Filho, Jurandir Marcondes Ribas; Mafafaia, Osvaldo; Czeczko, Leticia Elizabeth Augustin; Thiele, Edilson Schwansee; Aguiar, Luiz Roberto Farion de

doi:10.1590/s0100-69912009000200003

Cited by 2 publications

(2 citation statements)

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“…23 Mammals possess three pairs of major salivary glands: the parotid, submandibular and sublingual, all of which have a rich vascular plexus and nervous structure, surrounding the secretory and ductal components and relating directly to blood infiltrates. [24][25][26] However, although there are studies linking protozoan infections and the consequent impairment of the salivary glands, such as those associated with Trypanosoma cruzi, which causes Chagas disease, 27,28 research correlating Leishmania spp. infection and salivary glands in mammals are lacking in the literature, despite the predilection of the parasites in the salivary glands.…”

Section: Introductionmentioning

confidence: 99%

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Does Leishmaniasis disease alter the parenchyma and protein expression in salivary glands?

Júnior

Carvalho

Dantas

et al. 2015

Exp Biol Med (Maywood)

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Leishmaniasis is considered a serious public health problem in several regions in Brazil and worldwide. This research aimed to perform a histopathological and proteomic study of parotid, submandibular and sublingual glands of BALB/c mice infected by Leishmania (L) infantum chagasi using histological, immunohistochemical and epifluorescence techniques. Twelve isogenic BALB/ c male mice, around six-to eight-weeks old, were separated into two groups: the animals of the control group were injected with 0.15 ml of NaCl, while those in the experimental group were inoculated with 5 Â 10 6 amastigote forms of Leishmania (L) infantum chagasi by the ip route. After 50 days, animals were euthanized and major salivary glands were collected to perform histological, immunohistochemical and epifluorescence techniques using anti-Caspase-2, anti-Ki-67 and anti-b-catenin antibodies, respectively. The histological and morphometric evaluation showed clusters of mononuclear inflammatory cells and a higher area and perimeter of the parotid gland. However, none of the salivary glands had morphophysiological impairment. There was no immunoreactivity to the anti-caspase-2 antibody and Ki67 expression in acinar and ductal cells in both groups. According to the immunofluorescence staining, the b-catenin antibodies did not show nuclear expression, suggesting no uncontrolled proliferation. The data obtained in this study showed population and morphological stability of major salivary glands after 50 days post-infection by Leishmania (L) infantum chagasi.

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