Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)

Oliveira, Daria Pimenta de; Oliveira, Luiz Edson Mota de; Filho, Nelson Delú

doi:10.1590/s0100-67622006000500001

Cited by 7 publications

(5 citation statements)

References 19 publications

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“…Invertase activity (IA, U/mL) was determined using the method of de Oliveira et al . , with slight modifications. In particular, 0.1 mL of cell‐free culture medium was mixed with 0.9 mL of 0.05M phosphate buffer (Na 2 HPO 4 ‐NaH 2 PO 4 , pH 7) and 1% w/v sucrose.…”

Section: Methodsmentioning

confidence: 99%

Oleaginous yeast Cryptococcus curvatus exhibits interplay between biosynthesis of intracellular sugars and lipids

Tchakouteu

Chatzifragkou

Kalantzi

et al. 2014

Euro J Lipid Sci & Tech

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Patterns of the biosynthesis of major metabolites of the oleaginous yeast Cryptococcus curvatus NRRL Y-1511 were investigated during cultivation on sugar-based media. When lactose or sucrose was employed as substrate under nitrogen-limited conditions, the yeast strain accumulated high quantities of intra-cellular total sugars (ITS) at the beginning of fermentation (up to 68% w/w), with ITS values progressively decreasing to 20%, w/w, at the end of the fermentation. Decrease in ITS content and consumption of extracellular lactose led to a subsequent rise in lipid accumulation, reaching 29.8% in dry cell weight at 80 g/L of initial lactose concentration. Lactose was a more favorable substrate for lipid production than sucrose. In nitrogen-excess conditions, ITS were produced in significant quantities despite the continuous presence of nitrogen in the medium. Growth on lactose was not followed by secretion of extra-cellular b-galactosidase. High quantities of extra-cellular invertase were observed during growth on sucrose. The composition of ITS was highly influenced by the sugar used as substrate. Cellular lipids contained mainly palmitic and to lesser extent linoleic and stearic acids. This is the first report in the literature that demonstrates the interplay between the biosynthesis of intra-cellular total sugars and lipid synthesis for oleaginous yeast strains.Practical applications: Cryptococcus curvatus represents a promising candidate for the successful production of microbial lipids and polysaccharides during growth on several types of low-cost sugars utilized as substrates. Thus, C. curvatus could be used to produce a variety of metabolites useful for industrial biotechnology.

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Section: Methodsmentioning

confidence: 99%

Oleaginous yeast Cryptococcus curvatus exhibits interplay between biosynthesis of intracellular sugars and lipids

Tchakouteu

Chatzifragkou

Kalantzi

et al. 2014

Euro J Lipid Sci & Tech

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“…Physiological and molecular regulation of sucrose metabolism has been intensively studied in relation to rubber production in the laticifers of the Hevea trunk, which is the site tapped for latex ( Tupy, 1989 ; Dusotoit-Coucaud et al, 2010 ; Tang et al, 2010 ; Liu et al, 2015 , 2016 ). By comparison, very few studies are available with regard to sucrose metabolism in Hevea leaves, although the co-existence of VIN and NIN isoforms has been shown in both growing and developed Hevea leaves ( de Oliveira et al, 2006 ). Hevea leaf development has been investigated mainly with respect to leaf diseases ( Lieberei et al, 1989 ; Voss, 2001 ; Kursar and Coley, 2003 ; Lieberei, 2007 ), especially South American leaf blight (SALB), a fungal disease that has hampered Hevea planting in South and Central America.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Sugar Contents and Sucrose Metabolizing Enzymes in Developing Leaves of Hevea brasiliensis

Zhu

Fang

et al. 2018

Front. Plant Sci.

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Sucrose-metabolizing enzymes in plant leaves have hitherto been investigated mainly in temperate plants, and rarely conducted in tandem with gene expression and sugar analysis. Here, we investigated the sugar content, gene expression, and the activity of sucrose-metabolizing enzymes in the leaves of Hevea brasiliensis, a tropical tree widely cultivated for natural rubber. Sucrose, fructose and glucose were the major sugars detected in Hevea leaves at four developmental stages (I to IV), with starch and quebrachitol as minor saccharides. Fructose and glucose contents increased until stage III, but decreased strongly at stage IV (mature leaves). On the other hand, sucrose increased continuously throughout leaf development. Activities of all sucrose-cleaving enzymes decreased markedly at maturation, consistent with transcript decline for most of their encoding genes. Activity of sucrose phosphate synthase (SPS) was low in spite of its high transcript levels at maturation. Hence, the high sucrose content in mature leaves was not due to increased sucrose-synthesizing activity, but more to the decline in sucrose cleavage. Gene expression and activities of sucrose-metabolizing enzymes in Hevea leaves showed striking differences compared with other plants. Unlike in most other species where vacuolar invertase predominates in sucrose cleavage in developing leaves, cytoplasmic invertase and sucrose synthase (cleavage direction) also featured prominently in Hevea. Whereas SPS is normally responsible for sucrose synthesis in plant leaves, sucrose synthase (synthesis direction) was comparable or higher than that of SPS in Hevea leaves. Mature Hevea leaves had an unusually high sucrose:starch ratio of about 11, the highest reported to date in plants.

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“…Invertases were extracted by using procedure of Oliveira et al (2006). Activities of AI and NI were assayed following the methods of Jaleel et al (2007) and Wongmetha et al (2012).…”

Section: Activitiesmentioning

confidence: 99%

Key Regulators of Sucrose Accumulation during Different Developmental Phases of Sugar Beet Plants

Ali

2018

Egypt. J. Bot.

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B ECAUSE of the economic importance of sugar beet (Beta vulgaris L) in sucrose production, a field experiment was conducted to elucidate the key regulators of sucrose metabolism in source leaves and sink roots at 135, 150, 165 and 180 day after sowing (DAS) that would be determined sucrose yield. Increase in sucrose phosphate synthase (SPS) activity in source and sink tissues during the first three selected harvest phases was associated in leaves with an increase in activities of acid invertase (AI) and neutral invertase (NI), chlorophyll a, total chlorophyll and reducing sugars on the one hand and the levels of sucrose and inorganic phosphorus (P i ) in roots on the other hand. The maximum sucrose level in roots was at 165 DAS. A gradual increase in root NI activity accompanied by an increase in reducing sugars and a decrease in the sucrose/reducing sugars ratio in roots by harvest date progress was observed. Pearson correlation test revealed contradictory findings whereas, SPS activity correlated with sucrose level in leaves negatively but positively in roots. All investigated enzymes in leaves correlated negatively with sucrose/reducing ratio. In roots, only NI activity correlated negatively with sucrose/reducing sugars ratio.

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Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)

Cited by 7 publications

References 19 publications

Oleaginous yeast Cryptococcus curvatus exhibits interplay between biosynthesis of intracellular sugars and lipids

Oleaginous yeast Cryptococcus curvatus exhibits interplay between biosynthesis of intracellular sugars and lipids

Characterization of Sugar Contents and Sucrose Metabolizing Enzymes in Developing Leaves of Hevea brasiliensis

Key Regulators of Sucrose Accumulation during Different Developmental Phases of Sugar Beet Plants

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