Multiplicação in vitro de gemas axilares de acácia-negra (Acacia mearnsii De Wild.)

Júnior, Norton Borges; Soborsa, Rita de Cássia; Martins-Coder, Maisa Pimentel

doi:10.1590/s0100-67622004000400004

Cited by 11 publications

(4 citation statements)

References 10 publications

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“…Já MANTOVANI et al (1999) observaram que o segmento nodal de caixeta (Didymopanax morototoni) inoculado em meio acrescido de 1,0mg L -1 de BAP apresentou um maior alongamento de brotos. Para a acácia-negra (Acacia mearnsii), houve um efeito negativo no crescimento dos brotos quando utilizada a dose de 2,0mg L -1 de BAP (BORGES JÚNIOR et al, 2004).…”

Section: Resultsunclassified

Regeneração in vitro de Parapiptadenia rigida

et al. 2009

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Section: Resultsunclassified

Regeneração in vitro de Parapiptadenia rigida

et al. 2009

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“…Studying the in vitro propagation of Heliconia standleyi Macbride, Sosa-Rodriguez et al The beneficial action of BAP can be explained by the ability of plant tissue to metabolise natural hormones more quickly than synthetic hormones; it also has the lowest acquisition cost (BORGES JÚNIOR et al, 2004;ARAGON et al, 2011). Furthermore, BAP is one of the fastest absorbed of the cytokinins, acting quickly on plant metabolism and promoting the signalling of multiple shoot induction when grown in a culture medium (ROLLI et al, 2011;TAIZ and ZEIGER, 2013 Under suitable concentrations, the presence of BAP in the culture medium triggers activation of an enzyme complex in the plants, responsible for stimulating the initial vegetative growth of the lateral buds by promoting cell division (MURAI, 2014).…”

Section: Resultsmentioning

confidence: 99%

Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

Rodrigues

Santos

Carvalho

2016

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Considering the growing economic importance of tropical flowers and the advantages of techniques applied to the in vitro cultivation of these plants, it is necessary to carry out studies to evaluate growth in species such as Heliconia latispatha. The aim of this study therefore, was to evaluate in vitro shoot induction for different concentrations of BAP and NAA and as a function of the photoperiod. Explants from zygotic embryos were inoculated in MS medium containing different concentrations of BAP (0.0, 0.5, 1.0, 1.5, 2.0 or 2.5 mg L -1 ), with the cultures kept in a growth room at a temperature of 24.0 ± 2.0° C, under a photoperiod of 12 and 16 hours of light and a light intensity of 30 μmol m -2 s -1 . At 21, 28, 35, 42 and 49 days after inoculation, the number of shoots per explant was evaluated. The treatment at the BAP concentration that gave the best multiplication rate (2.5 mg L -1 ) was set, and was tested in a further trial with different concentrations of NAA (0.0, 0.2, 0.4, 0 6, 0.8 or 1.0 mg L -1 ) under the same conditions as the previous experiment. The experimental designs were completely randomised, with five replications, and analysed in a 6 x 2 factorial. The data were submitted to analysis of variance and regression. No significant differences were seen in relation to the photoperiod or its interaction with the cytokinin and auxin under test. Multiplication was greater in the presence of 2.5 mg L -1 BAP, which gave a rate of 1.25 shoots/explant at 49 days of in vitro culture. The association of this BAP dosage with 1.0 mg L -1 NAA was even more efficient, producing 1.83 shoots per explant at 30 days of growth. The use of BAP together with NAA is beneficial to the induction of shoots in H. latispatha. Keywords: Heliconiaceae, 6-benzylaminopurine, naphthaleneacetic acid, tissue culture. RESUMO Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimentoConsiderando a crescente importância econômica das flores tropicais e as vantagens das técnicas aplicadas ao cultivo in vitro para estas plantas, torna-se necessária a realização de estudos que avaliem a propagação de espécies como a Heliconia latispatha. Diante do exposto, o objetivo do trabalho foi avaliar a indução de brotações in vitro em função de diferentes concentrações de BAP e ANA em função de fotoperíodos. Os explantes, oriundos de embriões zigóticos, foram inoculados em meio MS contendo diferentes concentrações de BAP (0,0; 0,5; 1,0; 1,5; 2,0 ou 2,5 mg L -1 ), mantendo-se as culturas em sala de crescimento com temperatura de 24,0 ± 2,0 ºC sob fotoperíodo de 12 e 16 horas de luz e intensidade luminosa de 30 μmol m -2 s -1 . Aos 21, 28, 35, 42 e 49 dias após a inoculação, foi avaliado o número de brotos obtidos por explante. O tratamento com a concentração de BAP que proporcionou melhor taxa de multiplicação (2,5 mg L -1 ) foi fixado e, em outro ensaio foi testado com diferentes concentrações de ANA (0,0; 0,2; 0,4; 0,6; 0,8 ou 1,0 mg L -1 ) nas mesmas condições do experimento ...

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“…A formação de calo na base do explante reportado nesse estudo, também tem sido relatada por outros autores como um dos muitos fatores que interferem negativamente na taxa de multiplicação de espécies lenhosas, como Acacia mearnssii Wild. (Borges Júnior et al 2004), Peltophorum dubium (Spreng.) Taub.…”

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Conservação in vitro de Erythrina velutina a partir de embriões zigóticos

Fonseca

Nepomuceno

Silva

et al. 2015

Sitientibus sér. Ci. Biol.

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Erythrina velutina is native from the Caatinga domain, which has been heavily affected by the exploitation of natural resources. Thus, the aim of this study was to evaluate the induction of buds from nodal segments of in vitro germinated plants, aiming at the conservation of E. velutina. In the experiment I, intact seeds and zygotic embryos were inoculated in WPM culture and the number of buds, leaves and roots, germination percentage, shoot length and percentage of normal plants were analyzed. In the experiment II, the nodal segments of plants from germination of zygotic embryos in vitro were inoculated in WPM culture supplemented with BAP and CIN [0.0, 10.0, 20.0, 30.0 and 40.0 µM] and percentage of responsive explants to form buds, number of shoots and leaves, shoot length and the percentage of callus formation were analyzed. The cultivation of zygotic embryos provided more vigorous and uniform plants, with more gems than those obtained from the germination of intact seeds. The concentration of 20 µM of BAP is indicated for the regeneration of plants from zygotic embryos of E. velutina; with this concentration, an average of 2.78 shoots/explant was obtained.

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Multiplicação in vitro de gemas axilares de acácia-negra (Acacia mearnsii De Wild.)

Cited by 11 publications

References 10 publications

Regeneração in vitro de Parapiptadenia rigida

Regeneração in vitro de Parapiptadenia rigida

Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

Conservação in vitro de Erythrina velutina a partir de embriões zigóticos

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