Transmissão experimental revela novos potenciais reservatórios do Cowpea aphid-borne mosaic virus

Silva, Leonardo Assis da; Garcêz, Renata Maia; Chaves, Alexandre Levi Rodrigues; Colariccio, Addolorata; Eiras, Marcelo

doi:10.1590/s0100-54052012000200014

Cited by 6 publications

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“…Fruit hardening virus, caused by the Cowpea aphid-borne mosaic virus (CABMV) is a species of the genus Potyvirus (Cerqueira- Silva et al, 2014;Rodrigues et al, 2015). It is transmitted by aphids, grafting and mechanically by means of buffered leaf extract and occurs in the main producing areas of Brazil (SILVA et al, 2012;GARCÊZ et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea

et al. 2018

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Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs.

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Section: Introductionmentioning

confidence: 99%

Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea

et al. 2018

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“…The CABMV isolate was obtained from P. edulis (yellow passion fruit) produced in Monte Alegre do Sul, state of São Paulo, Brazil, and characterized according to Silva et al (2012). Sample leaves, collected in the field and stored in calcium chloride at -20ºC, were triturated with previously sterilized and chilled mortar and pestle containing a 1:5 (g:mL) sodium sulfite 0.5% medium (pH 6.0) (Gibbs & Harrison 1976) to obtain the viral inoculum.…”

Section: Methodsmentioning

confidence: 99%

Efeito do Cowpea aphid-borne mosaic virus sobre crescimento e variação quantitativa de fenois totais e flavonoides de Passiflora edulis Sims

et al. 2014

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Effect of Cowpea aphid-borne mosaic virus on growth and quantitative variation of total phenolics and flavonoid from Passiflora edulis Sims.) Cowpea aphid-borne mosaic virus induces woodiness of fruit pericarp, stunting, leaf mosaic and blistering in passion fruit plants. Total amount of phenols and flavonoids from leaves of health and artificially infected plants were quantified by the Folin-Ciocalteu method and reaction with aluminum chloride. Heights of all plants were measured at the beginning and end of the experiment. Infected plants presented 80% less height growth than healthy plants. There was no statistical difference in the amount of total phenols and flavonoids among treatments. Key words:Potyvirus, passion fruit woodiness disease, secondary metabolism.Resumo -(Efeito do Cowpea aphid-borne mosaic virus sobre crescimento e variação quantitativa de fenois totais e flavonoides de Passiflora edulis Sims.) Cowpea aphid-borne mosaic virus induz o endurecimento do pericarpo do fruto, nanismo, mosaico foliar e bolhas em plantas de maracujá. Os teores totais de fenois e flavonoides de folhas sadias e artificialmente infectadas foram quantificados pelo método de Folin-Ciocalteu e reação com cloreto de alumínio. Alturas de todas as plantas foram medidas no início e no final do experimento. As plantas infectadas apresentaram alturas 80% menores do que as plantas sadias. Não houve diferença estatística nos teores de fenois totais e de flavonoides entre tratamentos. Palavras-chave:Potyvirus, endurecimento dos frutos do maracujazeiro, metabolismo secundário, fenois, flavonoides.

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“…Apesar de o CABMV isoladamente provocar menores perdas na produção, a infecção simultânea com o CPSMV pode ocasionar sintomas mais severos, devido ao sinergismo entre esses vírus, ocasionando maiores perdas (27). Assim como mencionado para o CPSMV, a maneira mais eficiente para o controle do CABMV é a utilização de variedades resistentes (3, 9, 11,18,22,26). O estudo da base genética da resistência ao CPSMV e ao CABMV tem apontado, na maioria das vezes, para uma herança recessiva monogênica (1, 2, 26).…”

Section: Introductionunclassified

Obtenção de plantas de feijão-caupi resistentes ao Cowpea severe mosaic virus e ao Cowpea aphid-borne mosaic virus

et al. 2013

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RESUMOPalavras-chave adicionais: Vigna unguiculata, Comovirus, Potyvirus, melhoramento genético.Among the viruses tha t infect cowpea ( Vign a u n gu ic u lata L . Walp.), Cowpea severe mosaic virus (CPSMV) and Cowpea aphidb o rn e mo saic v iru s (CABMV) a re highlighted for their severity a n d wid esp rea d o ccu rr enc e, respe cti vel y. T h ere for e, the a im of t h is st u d y wa s t o o b t a i n a nd e v a l u a t e c o wpe a pl a n t s sh o wi n g resist a nce to CPS MV a nd CABMV in order t o develop n ew a nd e sse n t i a ll y d er i v e d c u l t iv a r s. E i g ht c r o sse s we r e pe r fo r me d , fo llo wed by ba c k cro sse s, u si ng t he lin e T E 9 7 -3 0 9 G-9 a n d t he cu l tiva r Pa ta tiva a s re sista nt pa rent a l do nors, a nd the c u ltiv a rs BR3-Tracuateua, BRS-Urubuquara, BRS-Novaera, BRS-Guariba and Pretinho a s su sceptible pa rents. Generations F2 a nd F2 RC1 were challenged for resista nce by mechanically inocu lating CPSMV a nd CABMV isolates. In F2RC1 genera tions, besides resista nce, other Barros, G.B.; Nogueira, M.S.R.; Oliveira, C.R.R.; Freire Filho, F.R.;Ribeiro, V.Q.; Veiga, C.F.M.; Brioso, P.S.T.; Eiras, M. Obtaining cowpea plants resistant to Cowpea severe mosaic virus and Cowpea aphid-borne mosaic virus. Summa Phytopathologica, v.39, n.2, p.130-136, 2013. traits were evaluated: number of days to the beginning of flowering, pod length, nu mber of seeds.pod-1, weight of 100 seeds, and yield of seeds.plant-1. All F2 and F2RC1 individuals were analyzed by the  2 test a nd fit to the expected frequency of 15 susceptible plants: 1 plant resistant to both viruses. The means of resistant F2RC1 plants, from each backcross, were compared with the mean of their respective recurrent parent by the t-test and means of backcrosses were compared by the Scott-Knott test. Genetic va riability among back crosses was detected for all traits. All backcrosses were considered promising for obta ining essentia lly derived cu ltiva rs resista nt to CPSMV a nd CABMV, and the selected plants have characteristics that allow the selection of lines with highly productive grains of good commercial quality.Dentre os vírus que infectam o feijão-caupi (Vigna ung uiculata L. Wa lp.) desta ca m-se, respectiva mente, pela severidade e ampla ocorrência o Cowpea severe mosaic virus (CPSMV) e o Cowpea aphidborne mosaic virus (CABMV). Portanto, objetivaram-se, no presente tra balho, obter e ava liar plantas de feijão-cau pi com resistência ao CPSMV e ao CABMV, visa ndo a o desenvolvimento de cu ltivares essencia lmente deriva da s e novas cu ltiva res. Rea liza ra m-se oito cruzamentos seguidos de retrocruzamentos, utilizando a linhagem TE 9 7 -30 9 G-9 e a cu ltiva r Pa ta tiva como genitores resistentes, e a s cu ltivares BR3 -Tra cua teu a, BRS-Urubu qua ra, BRS-Novaera, BRSGuariba e Pretinho como genitores suscetíveis. As gerações F 2 e F 2 RC 1 foram desafiadas quanto à resistência por meio de inoculação mecânica com isolados do CPSMV e do CABMV. Nas gerações F 2 RC 1 , além da resistência for...

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Transmissão experimental revela novos potenciais reservatórios do Cowpea aphid-borne mosaic virus

Cited by 6 publications

References 0 publications

Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea

Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea

Efeito do Cowpea aphid-borne mosaic virus sobre crescimento e variação quantitativa de fenois totais e flavonoides de Passiflora edulis Sims

Obtenção de plantas de feijão-caupi resistentes ao Cowpea severe mosaic virus e ao Cowpea aphid-borne mosaic virus

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