Atividade antimicrobiana de extratos peptídicos de folhas de berinjela na inibição do crescimento de Ralstonia solanacearum e Clavibacter michiganensis subsp. michiganensis

Almeida, Hebréia Oliveira; Teixeira, Felipe Roberti; Silva, Reginaldo Barboza da; Silva, Derly José Henriques da; Pereira, Paulo Roberto Gomes; Fontes, Elizabeth P. B.; Baracat‐Pereira, Maria Cristina

doi:10.1590/s0100-54052008000100012

Cited by 3 publications

(4 citation statements)

References 8 publications

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“…For fully expanded leaves, a Coomassie‐stained three‐phase SDS‐Tricine‐PAGE showed the presence of peptide in CWE‐CF2 and in SE‐CF1 and SE‐CF2 (Almeida et al., 2006). These previous results are consistent with the high antimicrobial activities observed for these fractions (Table 1).…”

Section: Discussionmentioning

confidence: 99%

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Peptide Fraction Inhibiting Plant Pathogen Growth Predominated in Cell Wall Extracts from Young Plants or in Soluble Cell Fraction from Expanded leaves from Eggplants

Almeida

Mattos

Barbosa

et al. 2007

Journal of Phytopathology

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Some varieties of eggplant are little susceptible to some plant pathogens, and antimicrobial peptides (AMPs) could be part of this innate-defence mechanism. This work aimed to evaluate the in vitro antimicrobial activity of peptide-enriched fractions from cell wall extracts (CWE) and soluble extracts (SE) from young plants and fully expanded leaves of eggplants against Ralstonia solanacearum and Clavibacter michiganensis ssp. michiganensis growth. The results indicated that the highest inhibition level of the multiplication of both bacteria was obtained with CWE from 5-cm-height plants, while SE promoted the highest inhibition rate when 15-cm-height plants or fully expanded leaves were analysed. These results suggest that young plants exhibit an innate-defence mechanism, very likely involving cell wall AMPs action to minimize plant antimicrobial invasion, while expanded leaves could produce soluble defence molecules. The inhibition rate was dependent for the extract concentration and the extracts were more effective against the growth of Gram-negative bacteria.

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Section: Discussionmentioning

confidence: 99%

“…michiganensis were assayed as plant pathogens. Micro‐organism’s origin, cultivation and maintenance were according to Teixeira et al (2006) and Almeida et al. (2007).…”

Section: Methodsmentioning

confidence: 99%

Peptide Fraction Inhibiting Plant Pathogen Growth Predominated in Cell Wall Extracts from Young Plants or in Soluble Cell Fraction from Expanded leaves from Eggplants

Almeida

Mattos

Barbosa

et al. 2007

Journal of Phytopathology

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“…CW proteins represent a subproteome of great importance, since many of these molecules are involved in the maintenance of the cellular structure and in processes of plant defense, for instance in responses to abiotic and biotic stresses ( Jamet et al , 2006 ; Kong et al , 2010 ), or as a constitutive barrier against pathogenic microorganisms. Studies in our laboratory evidenced high constitutive antimicrobial activity against two plant-pathogenic bacteria by the peptide fraction obtained from CW extracts from leaves of bell pepper ( Figure 2 , Teixeira et al , 2006 ) and 60-day-old eggplant ( Almeida et al , 2008 ). Also for eggplants, the highest inhibition levels were obtained with CW extracts from 5-cm-tall plants, while soluble extracts promoted the highest inhibition rate when fully expanded leaves were analyzed ( Table 1 ).…”

Section: Preparing and Isolating Low-abundance Proteins And Peptides mentioning

confidence: 94%

“…Plant CWs are highly dynamic and contain chemically active compounds secreted by the cells, which are essentially polysaccharides and proteins, the latter comprising approximately 10% of the CW mass ( Jamet et al , 2008 ). These proteins, which are difficult to isolate from these complex matrixes, require specific extraction methods ( Teixeira et al , 2006 ; Zhu et al , 2006 ; Almeida et al , 2007 , 2008 ; Kong et al , 2010 ), due to their low abundance. Differential extraction enriches the extract, allows access to the CW LAP, and facilitates the comparison of the expression profiles under different stress conditions ( Watson and Summer, 2006 ; Negri et al , 2008 ).…”

Section: Preparing and Isolating Low-abundance Proteins And Peptides mentioning

confidence: 99%

Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review

Baracat‐Pereira¹,

M²,

et al. 2012

Genet. Mol. Biol.

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The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of proteins involved in important physiological processes, such as plant defense against pathogens. This review discusses the challenges and strategies of separomics applied to the identification of low-abundance proteins and peptides in plants, especially in plants challenged by pathogens. Separomics is described as a group of methodological strategies for the separation of protein molecules for proteomics. Several tools have been used to remove highly abundant proteins from samples and also non-protein contaminants. The use of chromatographic techniques, the partition of the proteome into subproteomes, and an effort to isolate proteins in their native form have allowed the isolation and identification of rare proteins involved in different processes.

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Fitodefensivos em plantas medicinais: macromoléculas hidrofílicas de folhas de mil folhas (Achillea millefolium L.) inibem o crescimento in vitro de bactérias fitopatogênicas

Tessarollo¹,

Carrijo²,

Barbosa³

et al. 2013

Rev. bras. plantas med.

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RESUMO:Extratos aquosos da planta medicinal Achillea millefolium contêm macromoléculas de interesse para desenvolver fitodefensivos para a agricultura. Duas frações de mil folhas foram obtidas por ultrafiltração, E1 (contendo moléculas maiores que 30 kDa), e E3 (peptídeos entre 1 e 10 kDa) que inibiram o crescimento das bactérias fitopatogênicas Ralstonia solanacearum, gramnegativa, e Clavibacter michiganensis subsp. michiganensis, gram-positiva, com dependência de concentração. Os valores de concentração inibitória mínima (CIM) para ambos os extratos e bactérias foram baixos, entre 20 e 80mM. A CIM relativa à proteína total evidenciou a presença de macromoléculas muito ativas em E3, embora com baixa concentração proteica. E3 se aplica à prospecção de peptídeos antimicrobianos. Estimar a CIM relativa à quantidade de amostra vegetal valorizou o potencial antimicrobiano natural de E1, que contém alta concentração proteica. E1e E3 se aplicam ao desenvolvimento de fitodefensivos para uso biotecnológico. A ultrafiltração fracionou as amostras de forma nativa, rápida, e com baixo custo; além de dessalinizar, clarificar, purificar, e concentrar E1 e E3. Esse estudo inédito sobre a separômica e a ação antimicrobiana de extratos macromoleculares aquosos de mil folhas sugere que plantas cicatrizantes podem apresentar grande potencial para desenvolver fitodefensivos agrícolas naturais não danosos, à semelhança de medicamentos fitoterápicos.Palavras-chave: Macromoléculas de defesa, peptídeos antimicrobianos, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, concentração inibitória mínima relativa.ABSTRACT: Agrochemicals in medicinal plants: hydrophilic macromolecules from leaves of "mil folhas" (Achillea millefolium L.) inhibit in vitro growth of phytopathogenic bacteria. Aqueous extracts from the medicinal plant Achillea millefolium contain macromolecules of interest to develop agrochemicals for agriculture. Two fractions of "mil folhas" were obtained by ultrafiltration, E1 (containing molecules larger than 30 kDa) and E3 (peptides between 1 and 10 kDa), which inhibited the growth of phytopathogenic bacteria Ralstonia solanacearum, gramnegative, and Clavibacter michiganensis subsp. michiganensis, gram-positive, concentrationdependent. The values of minimum inhibitory concentration (MIC) for both extracts and both bacteria were low, ranging from 20 to 80mM. The MIC relative to total protein evidenced the presence of very active macromolecules in E3, although showing low protein concentration. E3 applies to the prospection of antimicrobial peptides. The estimated MIC relative to the amount of plant sample valued the natural antimicrobial potential of E1, which contains high protein concentration. E1 and E3 can be used in the development of agrochemicals for biotechnological purposes. The ultrafiltration procedure fractionated the samples in a rapid and native way and at a low cost; it also desalted, clarified, concentrated and purified both E1 and E3. This pioneering study on the separomics and on the a...

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Atividade antimicrobiana de extratos peptídicos de folhas de berinjela na inibição do crescimento de Ralstonia solanacearum e Clavibacter michiganensis subsp. michiganensis

Cited by 3 publications

References 8 publications

Peptide Fraction Inhibiting Plant Pathogen Growth Predominated in Cell Wall Extracts from Young Plants or in Soluble Cell Fraction from Expanded leaves from Eggplants

Peptide Fraction Inhibiting Plant Pathogen Growth Predominated in Cell Wall Extracts from Young Plants or in Soluble Cell Fraction from Expanded leaves from Eggplants

Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review

Fitodefensivos em plantas medicinais: macromoléculas hidrofílicas de folhas de mil folhas (Achillea millefolium L.) inibem o crescimento in vitro de bactérias fitopatogênicas

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