Peptide Fraction Inhibiting Plant Pathogen Growth Predominated in Cell Wall Extracts from Young Plants or in Soluble Cell Fraction from Expanded leaves from Eggplants

Almeida, Hebréia Oliveira; Mattos, Eliciane Cevolani; Barbosa, M.O.; Teixeira, Felipe Roberti; Magalhães, Rubens Daniel Miserani; Romeiro, R. S.; Fontes, Elizabeth P. B.; Baracat‐Pereira, Maria Cristina

doi:10.1111/j.1439-0434.2007.01308.x

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…We found that green-manured treatments suppressed the disease more and produced more yield than FYM-treated ones. Similar results were reported by other scientists (Ooshiro et al, 2004;Arthy et al, 2005;Matsushita et al, 2006;Olivier et al, 2006;Almeida et al, 2007;Posas et al, 2007;Acharya and Srivastava, 2009;Paret et al, 2010;Amorim et al, 2011;Yuan et al, 2012). Concentration of the soil amendments is however important.…”

Section: Survival Percentagesupporting

confidence: 88%

Evaluating Soil Amendments for the Management of Bacterial Wilt Disease of Tomato

Junaid¹,

Ahmad²,

Ullah³

2021

SJA

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Due broad range of host plants availability it is very difficult to control bacterial wilt disease completely. Soil fumigants tested by Murakoshi and Takahashi (1984) were found non-effective against bacterial wilt pathogen. Antibiotics are good but their applications are sometimes restricted and cannot be applied to field, tetracycline, penicillin, ampicillin and streptomycin have little effect (Farag et al., 1982). Biological control has also been investigated, but not satisfactory. In some previous researches Abstract | Two organic composts i.e. green manure (GM) and farmyard manure (FYM) were added to the soil at three concentrations i.e. C1= 1%, C2=5%, and C3=10%, to amend topsoil. Results revealed that increase in amendment's concentrations to 10% decreased the chance of plant survival to 68.06% while maximum (89.72%) number of plants survived for 1% amendments concentration. Significantly high number (87.22) of plants survived for green manure as compared to FYM with 78.47. C2 amended was found to be best with lowest (1.19) disease ratings. Significantly higher (i.e. 1.75 and 1.89) disease was observed for C1 and C3 amended soils. FYM was better as it produced significantly less (1.14) disease as compared to green manure with 1.35 disease severity. On the other hand, among concentrations, 5% was the best yielding with 2.17kg plant -1 , while the other concentrations i.e. 1% and 10% gave statistically similar and less yield (1.83 and 1.77kg plant -1 , respectively). Thus, it was concluded, that the application of green manure compost (used in 1% and 5% V/V concentration) to the soil, suppress the disease more and produced more yield than FYMtreated plants, however, the higher concentration i.e., 10% soil amendments had negative effect.

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Section: Survival Percentagesupporting

confidence: 88%

Evaluating Soil Amendments for the Management of Bacterial Wilt Disease of Tomato

Junaid¹,

Ahmad²,

Ullah³

2021

SJA

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“…chili ( Capsicum annum ) ( 117 ), Chinese gall ( Rhus chinensis ) ( 142 ), clove ( Szygyum aromaticum ) ( 11 ), cole ( Brassica sp.) ( 13 , 90 , 97 ), eggplant ( Solanum melongena ), ( 9 ), eucalyptus ( Eucalyptus globules ) ( 94 , 95 ), geranium ( Geranium carolinianum ) ( 91 ), guava ( Psidium guajava and P. quineense ) ( 3 ), hinoki ( Chamaecyparis obtusa ) ( 141 ), Japanese cedar ( Cryptomeria japonica ) ( 46 , 80 ), lemongrass ( Cimbopogon citratus ) ( 94 , 95 ), marigold ( Tagetes patula ) ( 118 ), neem ( Azadirachta indica ) ( 96 ), palmarosa ( Cimbopogon martint ) ( 94 , 95 ), pigeon pea ( Cajanus cajan ), sunn hemp ( Crotalaria juncea ) ( 20 ), tamarillo ( Cyphomandra betacea ) ( 92 ), thyme ( Thymus spp.) ( 53 , 99 ), wood wax tree ( Toxicodendron xylvestre ) ( 142 ), and worm killer ( Aristolochia bracteata ) ( 109 ).…”

Section: Methods Used For Crop Protectionmentioning

confidence: 99%

Recent Trends in Control Methods for Bacterial Wilt Diseases Caused by <i>Ralstonia solanacearum</i>

2015

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Previous studies have described the development of control methods against bacterial wilt diseases caused by Ralstonia solanacearum. This review focused on recent advances in control measures, such as biological, physical, chemical, cultural, and integral measures, as well as biocontrol efficacy and suppression mechanisms. Biological control agents (BCAs) have been dominated by bacteria (90%) and fungi (10%). Avirulent strains of R. solanacearum, Pseudomonas spp., Bacillus spp., and Streptomyces spp. are well-known BCAs. New or uncommon BCAs have also been identified such as Acinetobacter sp., Burkholderia sp., and Paenibacillus sp. Inoculation methods for BCAs affect biocontrol efficacy, such as pouring or drenching soil, dipping of roots, and seed coatings. The amendment of different organic matter, such as plant residue, animal waste, and simple organic compounds, have frequently been reported to suppress bacterial wilt diseases. The combined application of BCAs and their substrates was shown to more effectively suppress bacterial wilt in the tomato. Suppression mechanisms are typically attributed to the antibacterial metabolites produced by BCAs or those present in natural products; however, the number of studies related to host resistance to the pathogen is increasing. Enhanced/modified soil microbial communities are also indirectly involved in disease suppression. New promising types of control measures include biological soil disinfection using substrates that release volatile compounds. This review described recent advances in different control measures. We focused on the importance of integrated pest management (IPM) for bacterial wilt diseases.

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“…Folhas Teixeira et al (2006) e Almeida et al (2007; com modificações, na proporção 1:20 (g de folhas: mL de tampão de extração). As folhas (40g) trituradas foram maceradas em 50mM de TrisHCl, pH 7,0, contendo inibidores de proteases (10 mM de ácido etilenodiamino tetra-acético (EDTA), 1mM de fluoreto de fenilmetilsulfonila (PMSF), 1mM de benzamidina e 2mM de tiouréia).…”

Section: Materials Biológicounclassified

Fitodefensivos em plantas medicinais: macromoléculas hidrofílicas de folhas de mil folhas (Achillea millefolium L.) inibem o crescimento in vitro de bactérias fitopatogênicas

Tessarollo¹,

Carrijo²,

Barbosa³

et al. 2013

Rev. bras. plantas med.

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RESUMO:Extratos aquosos da planta medicinal Achillea millefolium contêm macromoléculas de interesse para desenvolver fitodefensivos para a agricultura. Duas frações de mil folhas foram obtidas por ultrafiltração, E1 (contendo moléculas maiores que 30 kDa), e E3 (peptídeos entre 1 e 10 kDa) que inibiram o crescimento das bactérias fitopatogênicas Ralstonia solanacearum, gramnegativa, e Clavibacter michiganensis subsp. michiganensis, gram-positiva, com dependência de concentração. Os valores de concentração inibitória mínima (CIM) para ambos os extratos e bactérias foram baixos, entre 20 e 80mM. A CIM relativa à proteína total evidenciou a presença de macromoléculas muito ativas em E3, embora com baixa concentração proteica. E3 se aplica à prospecção de peptídeos antimicrobianos. Estimar a CIM relativa à quantidade de amostra vegetal valorizou o potencial antimicrobiano natural de E1, que contém alta concentração proteica. E1e E3 se aplicam ao desenvolvimento de fitodefensivos para uso biotecnológico. A ultrafiltração fracionou as amostras de forma nativa, rápida, e com baixo custo; além de dessalinizar, clarificar, purificar, e concentrar E1 e E3. Esse estudo inédito sobre a separômica e a ação antimicrobiana de extratos macromoleculares aquosos de mil folhas sugere que plantas cicatrizantes podem apresentar grande potencial para desenvolver fitodefensivos agrícolas naturais não danosos, à semelhança de medicamentos fitoterápicos.Palavras-chave: Macromoléculas de defesa, peptídeos antimicrobianos, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, concentração inibitória mínima relativa.ABSTRACT: Agrochemicals in medicinal plants: hydrophilic macromolecules from leaves of "mil folhas" (Achillea millefolium L.) inhibit in vitro growth of phytopathogenic bacteria. Aqueous extracts from the medicinal plant Achillea millefolium contain macromolecules of interest to develop agrochemicals for agriculture. Two fractions of "mil folhas" were obtained by ultrafiltration, E1 (containing molecules larger than 30 kDa) and E3 (peptides between 1 and 10 kDa), which inhibited the growth of phytopathogenic bacteria Ralstonia solanacearum, gramnegative, and Clavibacter michiganensis subsp. michiganensis, gram-positive, concentrationdependent. The values of minimum inhibitory concentration (MIC) for both extracts and both bacteria were low, ranging from 20 to 80mM. The MIC relative to total protein evidenced the presence of very active macromolecules in E3, although showing low protein concentration. E3 applies to the prospection of antimicrobial peptides. The estimated MIC relative to the amount of plant sample valued the natural antimicrobial potential of E1, which contains high protein concentration. E1 and E3 can be used in the development of agrochemicals for biotechnological purposes. The ultrafiltration procedure fractionated the samples in a rapid and native way and at a low cost; it also desalted, clarified, concentrated and purified both E1 and E3. This pioneering study on the separomics and on the a...

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Peptide Fraction Inhibiting Plant Pathogen Growth Predominated in Cell Wall Extracts from Young Plants or in Soluble Cell Fraction from Expanded leaves from Eggplants

Cited by 7 publications

References 11 publications

Evaluating Soil Amendments for the Management of Bacterial Wilt Disease of Tomato

Evaluating Soil Amendments for the Management of Bacterial Wilt Disease of Tomato

Recent Trends in Control Methods for Bacterial Wilt Diseases Caused by <i>Ralstonia solanacearum</i>

Fitodefensivos em plantas medicinais: macromoléculas hidrofílicas de folhas de mil folhas (Achillea millefolium L.) inibem o crescimento in vitro de bactérias fitopatogênicas

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