Avaliação de diferentes meios de cultura na esporulação de Scytalidium lignicola

Carnaúba, Juliana Paiva; Sobral, Márcio Félix; Amorim, Edna Peixoto da Rocha; Silva, Júlio César da; Santos, Vanderley Borges dos; Félix, Kátia Cilene da Silva

doi:10.1590/s0100-54052007000200018

Cited by 4 publications

(1 citation statement)

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“…However, the media likely influence the selection some isolated species. According to Arnold et al ( 2000 ), tropical forests contribute substantially to fungal diversity, and considering statements by Carnaúba et al ( 2007 ), the media composition is a determining factor in fungal growth.…”

Section: Discussionmentioning

confidence: 98%

Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source

et al. 2013

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Endophytic fungi live inside plants, apparently do not cause any harm to their hosts and may play important roles in defense and growth promotion. Fungal growth is a routine practice at microbiological laboratories, and the Potato Dextrose Agar (PDA) is the most frequently used medium because it is a rich source of starch. However, the production of potatoes in some regions of the world can be costly. Aiming the development of a new medium source to tropical countries, in the present study, we used leaves from the guarana (a tropical plant from the Amazon region) and the olive (which grows in subtropical and temperate regions) to isolate endophytic fungi using PDA and Manihot Dextrose Agar (MDA). Cassava (Manihot esculenta) was evaluated as a substitute starch source. For guarana, the endophytic incidence (EI) was 90% and 98% on PDA and MDA media, respectively, and 65% and 70% for olive, respectively. The fungal isolates were sequenced using the ITS- rDNA region. The fungal identification demonstrated that the isolates varied according to the host plant and media source. In the guarana plant, 13 fungal genera were found using MDA and six were found using PDA. In the olive plant, six genera were obtained using PDA and 4 were obtained using MDA. The multivariate analysis results demonstrated the highest fungal diversity from guarana when using MDA medium. Interestingly, some genera were isolated from one specific host or in one specific media, suggesting the importance of these two factors in fungal isolation specificity. Thus, this study indicated that cassava is a feasible starch source that could serve as a potential alternative medium to potato medium.

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Section: Discussionmentioning

confidence: 98%

Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source

et al. 2013

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Produção de esporos e efeito da temperatura e luminosidade sobre germinação e infecção de Pseudocercospora vitis em videira

et al. 2015

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RESUMO Dentre as doenças fúngicas da videira, merece destaque a mancha-da-folha causada por Pseudocercospora vitis. As condições favoráveis ao desenvolvimento da mancha-da-folha ainda não foram totalmente determinadas. Sabe-se que a formação de conídios ocorre sob condições de alta umidade e são disseminados pela ação do vento, iniciando novas infecções. Para aumentar o conhecimento sobre a produção, germinação e infecção do patógeno foram realizados três experimentos. No primeiro experimento, avaliou-se a produção de esporos de P. vitis, nos meios de cultura: BDA (batata-ágar-dextrose); BDA+Leite de coco (5%); BDA+extrato de uva cv. Isabel (5%); ST (suco de tomate-carbonato de cálcio-ágar); ST+leite de coco (5%); ST+extrato de uva cv. Isabel (5%). Houve esporulação em todos os meios testados e diferentes períodos de incubação. No segundo experimento, verificou-se que a temperatura de 25 °C propiciou maior porcentagem de conídios germinados e que houve maior germinação na ausência de luz. No terceiro experimento observou-se que a temperatura de 20 °C propiciou maior severidade da mancha-da-folha em mudas de videiras, após 48 horas de incubação no escuro.

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Utilização do extrato aquoso de cinamomo no controle da antracnose da videira

2012

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RESUMOPalavras-chave adicionais: cinamomo, agroecologia, extratos vegetais, produção orgânicaThe aim of this study was to evaluate the effect of aqueous extract of chinaberry (Melia azedarach L.) on Elsinoe ampelina, the etiological agent of grapevine anthracnose, and on the disease control. For the trials of mycelial growth, sporulation and conidium germination, the concentrations 0, 10, 20, 30, 40 and 50 mL L -1 extract were used, besides standard treatments with bordeaux mixture and mancozeb. Under field conditions, an experiment was conducted in a commercial vineyard for two consecutive cycles (2009/2010, 2010/2011) to evaluate increasing concentrations of extract and vegetable oil (2.5 mL L -1 ), in addition to an absolute control (without treatment) and standard treatment with bordeaux mixture. Concentrations equal to or higher Silva, C.M.; Botelho, R.V.; Faria, C.M.R.D. Use of aqueous extract of chinaberry to control grapevine anthracnose. Summa Phytopathologica, v.38, n.4, p.312-318, 2012. than 20 mL L -1 extract led to complete inhibition of sporulation. The concentration of 50 mL L -1 decreased by 99.4% the colony diameter and did not differ from the treatment with bordeaux mixture, besides reducing conidium germination by 84.8 and 90.8%, compared to control, at 12 and 24 hours after incubation. In the first year of the experiment in the field, there was a negative linear effect of extract concentrations on anthracnose severity. However, in the second year, the use of vegetable oil as adjuvant masked the extract effect. The isolate application of vegetable oil reduced AUDPC by 64.0%, which is similar to the results obtained with all concentrations of chinaberry extract and the standard treatment with bordeaux mixture.O objetivo desse trabalho foi avaliar o efeito do extrato aquoso de cinamomo (Melia azedarach L.) sobre Elsinoe ampelina, agente etiológico da antracnose da videira, e no controle da doença. Para os experimentos de crescimento micelial, esporulação e geminação de conídios do fungo foram utilizadas as concentrações de 0, 10, 20, 30, 40 e 50 mL L -1 de extrato, além dos tratamentos padrões com calda bordalesa e mancozebe. Em condições de campo, um experimento foi conduzido em vinhedo comercial por dois ciclos consecutivos (2009/2010, 2010/2011) Additional keywords: chinaberry, agroecology, plant extracts, organic production. ABSTRACTA videira é economicamente uma das mais importantes fruteiras cultivadas no mundo, devido às inúmeras utilizações dos seus frutos para consumo in natura e processamento. As doenças fúngicas constituem-se como sendo um dos principais problemas de interesse econômico na viticultura, em consequência das perdas registradas. Conforme Sônego & Garrido (28), dentre as principais doenças fúngicas, destaca-se a antracnose, causada por Elsinoe ampelina (de Bary) Shear e responsável por grandes danos para a viticultura no sul do Brasil, assim como em outras regiões vitícolas do mundo.A antracnose da videira é uma doença de origem européia que reduz a qualidade e a quan...

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Avaliação de diferentes meios de cultura na esporulação de Scytalidium lignicola

Cited by 4 publications

References 2 publications

Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source

Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source

Produção de esporos e efeito da temperatura e luminosidade sobre germinação e infecção de Pseudocercospora vitis em videira

Utilização do extrato aquoso de cinamomo no controle da antracnose da videira

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