Preservação de urediniósporos de Puccinia melanocephala, agente causal de ferrugem em cana-de-açúcar

Oliveira-Garcia, Ely; Casagrande, Marcos V.; Rago, Alejandro; Júnior, Nelson Sidnei Massola

doi:10.1590/s0100-54052007000200009

Cited by 15 publications

(10 citation statements)

References 7 publications

(9 reference statements)

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“…Therefore, an effective uredospore preservation method would facilitate plant pathology assays. Several methodologies for the preservation of uredospores are described in the literature for other rusts, including wheat (Bromfield, 1964), sugarcane (Garcia et al, 2007) and bean rust (Faleiro et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Preservation of Phakopsora pachyrhizi Uredospores

Furtado

Alves

Czermainski

et al. 2007

Journal of Phytopathology

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This study compared different temperatures and dormancy-reversion procedures for preservation of Phakopsora pachyrhizi uredospores. The storage temperatures tested were room temperature, 5°C, )20°C and )80°C. Dehydrated and non-dehydrated uredospores were used, and evaluations for germination (%) and infectivity (no. of lesions⁄cm 2 ) were made with fresh harvested spores and after 15, 29, 76, 154 and 231 days of storage. The dormancy-reversion procedures evaluated were thermal shock (40°C⁄5 min) followed or not by hydration (moist chamber⁄24 h). Uredospores stored at room temperature were viable only up to a month of storage, regardless of their hydration condition. Survival of uredospores increased with storage at lower temperatures. Dehydration of uredospores prior to storage increased their viability, mainly for uredospores stored at 5°C, )20°C and )80°C. At 5°C and )20°C, dehydrated uredospores showed increases in viability of at least 47 and 127 days, respectively, compared to non-dehydrated spores. Uredospore germination and infectivity after storage for 231 days (7.7 months), could only be observed at )80°C, for both hydration conditions. At this storage temperature, dehydrated and non-dehydrated uredospores exhibited 56 and 28% of germination at the end of the experiment, respectively. Storage at )80°C also maintained uredospore infectivity, based upon levels of infection frequency, for both hydration conditions. Among the dormancyreversion treatments applied to spores stored at )80°C, those involving hydration allowed recoveries of 85 to 92% of the initial germination.

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Section: Introductionmentioning

confidence: 99%

Preservation of Phakopsora pachyrhizi Uredospores

Furtado

Alves

Czermainski

et al. 2007

Journal of Phytopathology

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“…Os resultados do bioensaio corroboram com os resultados alcançados por outros autores (5, 6, 9, 10, 15, 17, 19, 20) para preservação de fungos, parasitas obrigatórios de plantas. Teste semelhante a este foi realizado com urediniósporos de P. melanocephala, no qual os autores observaram que a preservação dos urediniósporos a baixas temperaturas (-20ºC e -80ºC), antecedidos de desidratação em sílica gel, manteve a viabilidade por um período de até um ano de armazenamento (10).…”

Section: Resultado E Discussão Preservação De Urediniósporos De Pucciunclassified

Métodos de preservação in vitro de urediniósporos de Puccinia kuehnii

et al. 2012

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RESUMOPalavras-chave adicionais: ferrugem alaranjada, liofilização, sílica gel, Saccharum officinarum, reidratação.With the aim of evaluating preservation methods of Puccinia kuehnii urediniospores, two bioassays were conducted: the first one (B1) with different methods of dehydration and the second one (B2) with different methods rehydration. B1 and B2 differed in the inclusion of silica gel granule in B1 for preservation in microcentrifuge tubes. Leaves with symptoms of orange rust were harvested from the sugarcane cultivar SP89 1115. P. kuehnii urediniospores were extracted from the leaves with the aid of a vacuum bomb. Later, they were placed in microcentrifuge tubes. Treatments for B1 were: l: dehydration in silica gel, lyophilization and without dehydration, ll: room temperature (20°C), refrigerator (5°C), freezer (-20°C) and deep-freezer (-80°C). Treatments for B2 were: l: dehydration in silica gel and without dehydration, ll: room temperature (20°C), refrigerator (5°C), freezer (-20°C) and deep-freezer (-80°C), lll: with and without rehydration in the evaluations. The initial germination was carried out for both experiments, other assessments were made at 15 and 30 days of storage and then at every 30 days until 180 Tibolla, F.; Sumida, C.H.; Peitl, D.C.; Canteri, M.G., Castro, A.M.C. In vitro preservation methods of Puccinia kuehnii urediniospores. Summa Phytopathologica, v.38, n.3, p.198-203, 2012. days. Urediniospore suspensions were prepared in water and a 0.1-mL aliquot was transferred to Petri dishes containing agar-water (15 g L -1 ). They remained at 20°C in the dark. To assess viability, the count of 200 urediniospores/plate was performed. The data were subjected to nonparametric Kruskal-Wallis analysis of variance and complemented by Dunn's test. Results showed that viability decreased with time, and the best treatments reached 27.6% and 6.6% at 30 days, and 12.0% and 1.9% at 60 days for B1 and B2, respectively. Dehydration method in silica gel followed by storage at -80°C was the only treatment that presented viable urediniospores (1.2%) at 180 days for B1. For B2, the best method was preservation with dehydration, followed by storage at 5°C, without rehydration. This method showed the best germination percentage at 120 days (0.4%). Influence of the factor thermal shock was not observed in the recovery of viable urediniospores in B2. Inclusion of silica gel granules in the microcentrifuge tube allowed the recovery of viability of urediniospores at 180 days.Com o objetivo de avaliar métodos de preservação de urediniósporos de Puccinia kuehnii, conduziram-se dois bioensaios sendo o primeiro (B1) com diferentes métodos de desidratação e o segundo (B2), com diferentes métodos de reidratação. Em B1 foi adicionado um grânulo de sílica gel para preservação dos urediniósporos nos tubos de microcentrífuga. Foram coletadas folhas com sintomas de ferrugem alaranjada, P. kuehnii, da cultivar de cana-de-açúcar SP89 1115. Os urediniósporos do agente causal de ferugem foram extraídos das folhas com o auxí...

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“…Individual buds were planted in 3-L pots containing a 3:1:1 mixture of soil, sand, and substrate (pine and coconut bark-Tropstrato) and grown in a greenhouse until inoculation. Initially, 30-day-old plants were kept in a humid growth chamber for 12 h. Subsequently, the abaxial face of leaves was sprayed with a solution of 2 x 10 4 urediniospores/mL from each of the 3 regions (Garcia et al, 2007). After inoculation, samples were kept in humid growth chamber for 24 h. Next, plants remained protected in a chamber, separated by regions to enable spore maintenance and multiplication.…”

Section: Evaluation Of Aggressiveness Of P Melanocephalamentioning

confidence: 99%

“…To evaluate differences in aggressiveness among P. melanocephala isolates, inoculations were conducted on excised leaves (Garcia et al, 2007). The abaxial side of a 20-cm segment of the +1 leaf of cultivars 'SP701143' (susceptible) and 'RB835486' (intermediately resistant) was sprayed with a solution of 2 x 10 4 viable urediniospores/mL.…”

Section: Evaluation Of Aggressiveness Of P Melanocephalamentioning

confidence: 99%

Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers

Peixoto-Junior¹,

Creste²,

Landell³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Brown rust (causal agent Puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. Despite its importance, little is known regarding the genetic diversity of this pathogen in the main Brazilian sugarcane cultivation areas. In this study, we characterized the genetic diversity of 34 P. melanocephala isolates from 4 Brazilian states using loci identified from an enriched simple sequence repeat (SSR) library. The aggressiveness of 3 isolates from major sugarcane cultivation areas was evaluated by inoculating an intermediately resistant and a susceptible cultivar. From the enriched library, 16 SSR-specific primers were developed, which produced scorable alleles. Of these, 4 loci were polymorphic and 12 were monomorphic for all isolates evaluated. The molecular Genetic diversity among Puccinia melanocephala isolates characterization of the 34 isolates of P. melanocephala conducted using 16 SSR loci revealed the existence of low genetic variability among the isolates. The average estimated genetic distance was 0.12. Phenetic analysis based on Nei's genetic distance clustered the isolates into 2 major groups. Groups I and II included 18 and 14 isolates, respectively, and both groups contained isolates from all 4 geographic regions studied. Two isolates did not cluster with these groups. It was not possible to obtain clusters according to location or state of origin. Analysis of disease severity data revealed that the isolates did not show significant differences in aggressiveness between regions.

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Preservação de urediniósporos de Puccinia melanocephala, agente causal de ferrugem em cana-de-açúcar

Cited by 15 publications

References 7 publications

Preservation of Phakopsora pachyrhizi Uredospores

Preservation of Phakopsora pachyrhizi Uredospores

Métodos de preservação in vitro de urediniósporos de Puccinia kuehnii

Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers

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