Flavonoids extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different procedures

Abstract

Abstract:The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Alpinia zerumbet. Dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. By the application of TLC and reversed-phase HPLC techniques the rutin and kaempferol-3-O-glucuronide were detected. Ethanol 70% was more efficient for flavonoids extraction than water. No significant yielding variation was verified for ultrasonic, microwave and stirring methods using ethanol 70% (11 to 14%). Relative concentration of rutin and kaempferol-3-Oglucuronide, respectively, was higher by ultrasonic (1.5 and 5.62 mg g -1 dried leaves) and by microwave (1.0 and 6.64 mg g -1 dried leaves) methods using 70% ethanol. Rapid and simplified extraction proceeding optimize phytochemical work and acquisition of secondary metabolites.

Antibacterial activity of Lemna minor extracts against Pseudomonas fluorescens and safety evaluation in a zebrafish model

“…For the evaluation of these extracts, six aqueous concentrations were prepared from 5000 µg mL −1 to 0.05 µg mL −1 per extraction method. The extraction yield was calculated by the formula (crude extract weight/plant material weight) × 100 ( Victório et al, 2010 ). To prepare the aqueous extracts, with the methanolic extract, water was added and polyvinylpyrrolidone (PVP) (Sigma-Aldrich®) at proportion of 1:4 (crude extract: PVP, (w/w), was used to allow to make a miscible solution with hexane and chloroform extracts.…”