Inhibitors of human collagenase, MMP1

Leite, Sérgio Roberto de Andrade

doi:10.1590/s0100-46702009000400008

Cited by 6 publications

(3 citation statements)

References 27 publications

(35 reference statements)

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“…Since increased MMP1 activity was observed in the BBBdeficit groups, we sought to test whether inhibition of MMP1 can restore BBB function in the BBB-deficit group. Piroxicam is a non-steroidal anti-inflammatory drug that broadly inhibits several MMPs but is highly selective for MMP1 compared to other MMPs 46 . Infliximab is an immunosuppressive drug that specifically targets TNFα 47 and has been shown to decrease MMP1 levels, endothelial cell necroptosis, and to prevent BBB leakage 48 .…”

Section: Mmp1 Impairs Bbb Functionmentioning

confidence: 99%

TNFα and MMP1 in brain microvascular endothelial cells regulate blood-brain barrier dysfunction in psychotic disorders

Lizano¹,

Pong²,

Santarriaga

et al. 2022

Preprint

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In schizophrenia and bipolar disorder, tight junction deficits in brain microvascular endothelial cells (BMECs) lead to blood brain barrier (BBB) dysfunction. Using an in vitro model of human BBB function, we show that BMECs derived from human induced pluripotent stem cells of schizophrenia patients show reduced BBB integrity and increased small molecule permeability when compared to healthy control BMECs. Stratification based on BBB function in schizophrenia and bipolar disorder patients identified a BBB-deficit subtype with reduced barrier function, increased permeability to larger molecules, and decreased claudin-5 (CLDN5) levels. BMECs from the BBB-deficit group show increased MMP1 activity, which correlated with reduced CLDN5 levels and worse BBB function, which were rescued by tumor necrosis factor α (TNFα and MMP1 inhibition. These results show intrinsic deficits in BMECs in psychotic disorders that result in BBB disruption and further identify TNFα and MMP1 as potential targets for ameliorating BBB deficits.

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Section: Mmp1 Impairs Bbb Functionmentioning

confidence: 99%

TNFα and MMP1 in brain microvascular endothelial cells regulate blood-brain barrier dysfunction in psychotic disorders

Lizano¹,

Pong²,

Santarriaga

et al. 2022

Preprint

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“…Lower IL2 concentration is responsible for decreased T cell proliferation, B cell multiplication and differentiation, reduced monocyte and K cell activity [14]. The K and NK cells perform cell recognition and destruction not dependent on MHC class I [15]. NK cells destroy virus-infected cells or tumour cells.…”

Section: Introductionmentioning

confidence: 99%

Considerations on the Influence of Zinc on Infectious Diseases in Children

Negruț¹,

Rus²,

Pantiş³

et al. 2020

Rev. Chim.

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Zinc modulates the human body defence against infections. Mild and medium deficiency in this mineral appears usually sub-clinically, being mistaken for other diseases, but the severe form can be fatal. The purpose of the study was to determine the plasma zinc concentration (PZC) for the most common infectious pathology in children. Zinc was measured in plasma using direct colorimetric assay based on the 5-Br-PAPS method (CV% 0.98-4.64%). In the paediatric patients, 0-3 years old, the PZC values were 15.20�1.37 μmol/L, with limits ranging between 13.05-20.6 μmol/L, the values falling within normal limits and proving the absence of zinc deficiency in the investigated population. During 3 years of follow up, 137 healthy children presented low values of plasma zinc concentration if they had acute lower respiratory infections, acute otitis media or giardiasis in past medical history. There were not found significant differences in case of children with viral or bacterial acute diarrheal diseases or viral exanthemas. In the present study, the children exposed to severe, complicated or chronic forms of infectious diseases were predisposed to low plasma zinc concentrations.

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“…Latency of collagenase is removed to form the active enzyme by proteolytic enzymes such as trypsin and non proteolytic agents such as chatropic salts and organo mercurial. The conversion of latent enzyme to its active enzyme is accompanied by a decrease in its molecular weight of about (10-20) kilo dalton (Leite et al, 2009;Meada et al, 1995;Smith et al, 1989). The difference in the molecular weight of MMPs is depend on their type (serine or metalloproteinase) and their source (animal or microbial) (Daboor et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Isolation of Latent Matrix Metallo Proteinase-1 (Latent Collagenase-1) from Serum and Synovial Fluid of Rheumatoid Arthritis Patient

Hamoudatt¹,

Chemistry²,

Mustafa³

2013

RJS

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This study included the isolation of the enzyme Matrix Metalloprotrinase-1 (MMP-1; Collagenase-1) from serum of healthy individual and synovial fluid (SF) of a patient with rheumatoid arthritis using different techniques. After precipitation of proteins using saturated ammonium sulfate, two porteinous components had been isolated by gel filtration chromatography. It was found that only the first peak has high activity of latent-MMP-1. The apparent molecular weights of MMP-1 in serum and SF using gel filtration chromatography was found to be (47752 ± 816 and 48194 ± 707) dalton respectively. High performance liquid chromatography (HPLC) was used to show the extent of purity. The main maximum of the enzyme from serum and SF appeared at retention times (1.652 and 1.65) minutes respectively compared with the retention time of standard enzyme at (1.571) minutes. The approximate molecular weight of latent-MMP-1 by HPLC technique, in serum and SF were found (48067 and 48033) dalton respectively. The study included, also, the effect of some material compounds on the activity of latent-MMP-1. The results revealed that the addition of ethylene diamine tetra acetic acid (EDTA), 2,4-dinitrophenol, sodium azide, potassium oxalate, mercaptoethanol (MEH), glutathione (GSH), cystein and healthy human serum decreased the activity of latent-MMP-1, while the addition of NaCl, MgSO 4 , CoCl 2 , CaCl 2 , CuSO 4 , ZnSO 4 , HgCl 2 and pepsin increases the activity of enzyme.

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Inhibitors of human collagenase, MMP1

Cited by 6 publications

References 27 publications

TNFα and MMP1 in brain microvascular endothelial cells regulate blood-brain barrier dysfunction in psychotic disorders

TNFα and MMP1 in brain microvascular endothelial cells regulate blood-brain barrier dysfunction in psychotic disorders

Considerations on the Influence of Zinc on Infectious Diseases in Children

Isolation of Latent Matrix Metallo Proteinase-1 (Latent Collagenase-1) from Serum and Synovial Fluid of Rheumatoid Arthritis Patient

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