Determinação de cádmio em amostras de urina e soro humano por espectrometria de absorção atômica em forno de grafite usando irídio como modificador permanente

Pinto, Francisco de Assis de Carvalho; Rey, Ulisses Vilela; Ansaloni, L. M. S.; Andrada, Daniel; Silva, J. B. B.

doi:10.1590/s0100-46702005000400008

Cited by 3 publications

(4 citation statements)

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“…Intra-and inter-assay precision was below the recommended values found in other studies for both analytes. [22][23][24]30 In this study we successfully demonstrated that there was no need for lengthy digestion prior to the analysis, which is subject to losses and contamination. Finally, the use of a permanent modier ensures the destruction of the matrix in situ providing the use of aqueous calibration, i.e.…”

Section: Discussionmentioning

confidence: 90%

“…The condition without the use of a modier was also studied. The lifetime of the graphite tube using a permanent modier without a decrease in sensitivity is more than 1000 cycles, 24,25 but was not investigated in this study.…”

Section: Methodsmentioning

confidence: 99%

“…All other tested modiers resulted in very low relative sensitivity. The diluent was selected as described by Pinto et al 24 in human serum and based on the results obtained for Bi, Cr and Al determinations in human serum samples. [27][28][29] Multivariate optimization…”

Section: Choice Of Diluents and Modiersmentioning

confidence: 99%

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Development and validation of methods for the determination of copper and iron in serum of dogs with canine visceral Leishmaniasis using multivariate optimization and GF AAS

et al. 2013

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In this work efficient methods to determine copper and iron in dog serum samples by graphite furnace atomic absorption spectrometry were developed. The samples were diluted at a 1 : 9 or 1 : 19 (for Cu and Fe, respectively) ratio with 1% (v/v) nitric acid containing a 0.1% Triton X-100 solution. Rhodium for copper and no modifier for iron proved to be the better permanent modifier. Optimization included fractional factorial planning using Pareto and the CCD designs. For both analytes the working linear range was 0-100 mg L À1 (r 2 > 0.99). The obtained LOQ was 19.3 AE 2.8 mg L À1 for Cu and 16.5 AE 0.2 mg L À1 for Fe. Aqueous and matrix matching calibration curves had average angular coefficients that were not statistically different, i.e. the matrix effect was absent for both analytes. The accuracy was checked by recovery tests with an average of 101 AE 4% (n ¼ 45) for Cu and 90 AE 3% for Fe (n ¼ 45). The certified reference material SeronormÔ Trace Elements Serum L-1 obtained was 1705 AE 20 mg L À1 for Cu (certified: 1691 AE 84 mg L À1 ) and 1.40 AE 1.22 mg L À1 for Fe (certified: 1.43 AE 0.08 mg L À1 ). Cu and Fe levels in 39 canine serum samples -9 uninfected, 19 symptomatic and 11 asymptomatic dogs naturally infected with Leishmania chagasiranged from 374 to 913 mg L À1 , and 1103 to 4260 mg L À1 , respectively. Cu was higher in symptomatic than in asymptomatic and control dogs, and Fe was significantly lower in symptomatic than in either asymptomatic or control dogs.

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Section: Discussionmentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

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Development and validation of methods for the determination of copper and iron in serum of dogs with canine visceral Leishmaniasis using multivariate optimization and GF AAS

et al. 2013

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“…The condition without modifier was also studied. The lifetime of the graphite tube using a permanent modifier without a decrease in sensitivity is more than 1000 cycles (11,(12)(13)(14); however, this was not investigated in this study.…”

Section: Experimental Instrumentationmentioning

confidence: 99%

Development and Validation of Methods for the Determination of Aluminum and Lead in Beer Using Multivariate Optimization and GFAAS

Oliveira¹

2016

At.Spectrosc.

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In this work, efficient methods to determine aluminum (Al) and lead (Pb) in beer samples by graphite furnace atomic absorption spectrometry (GFAAS), following multivariate optimization, were developed. The only sample preparation used was degassing of the beer to remove CO 2. Permanent rhodium (Rh) for aluminum and permanent iridium (Ir) for lead proved to be the better permanent modifier. Optimization included fractional factorial planning using the Pareto and the CCD designs. The working linear range was 0-90 µg L-1 for aluminum and 0-60 µg L-1 for lead (r 2 >0.99). The obtained LOQs were 4.8 and 1.6 µg L-1 for aluminum and lead, respectively. Aqueous and matrix-matched calibration curves had average angular coefficients that were not statistically different, i.e., the matrix effect was absent for aluminum, while for lead the coefficients were statistically different. Matrix-matched calibration was used in the subsequent studies for the beer samples. The accuracy was checked by recovery tests with a percentage recovery range of 89-95% (n=45) for Al and 97 to 98% for Pb (n=45). The coefficient of variation (CV) of the intra-assay studies (n=15) was 4.0 and 2.5% for Al and Pb, respectively, where the CV for the inter-assay studies (n=45) was 3.0 for Al and 3.1% for Pb. The Al and Pb levels in the 21 beer samples varied from

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Application of constrained mixture design and Doehlert matrix in the optimization of dispersive liquid-liquid microextraction assisted by ultrasound for preconcentration and determination of cadmium in sediment and water samples by FAAS

Meira

Dias

2017

Microchemical Journal

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Determinação de cádmio em amostras de urina e soro humano por espectrometria de absorção atômica em forno de grafite usando irídio como modificador permanente

Cited by 3 publications

References 3 publications

Development and validation of methods for the determination of copper and iron in serum of dogs with canine visceral Leishmaniasis using multivariate optimization and GF AAS

Development and validation of methods for the determination of copper and iron in serum of dogs with canine visceral Leishmaniasis using multivariate optimization and GF AAS

Development and Validation of Methods for the Determination of Aluminum and Lead in Beer Using Multivariate Optimization and GFAAS

Application of constrained mixture design and Doehlert matrix in the optimization of dispersive liquid-liquid microextraction assisted by ultrasound for preconcentration and determination of cadmium in sediment and water samples by FAAS

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