Estudos histopatológicos de Colletotrichum spp. em plântulas de cafeeiro

Lins, Severina R.O.; Abreu, Márvio Lobão Teixeira de; Alves, Eduardo

doi:10.1590/s0100-41582007000600006

Cited by 17 publications

(12 citation statements)

References 14 publications

(13 reference statements)

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“…In this study, conidial germination was observed 6 h.a.i, through the formation of the germ tube or appressoria, similar to the findings obtained with C. dematium in cowpea and C. fragariae in cherimoya fruits (SMITH; KORSTEN; AVELING, 1999;VILLANUEVA-ARCE et al, 2006). In coffee plants inoculated with C. gloeosporioides and C. dematium, the conidial germination started 5 and 12 h.a.i, respectively (LINS; ABREU; ALVES, 2007). Conidia of Colletotrichum acutatum germinated 4 h.a.i on strawberries (ARROYO et al, 2005) and C. destructivum took 12 h to germinate on tobacco (SHEN; GOODWIN; HSING, 2001).…”

Section: Suspensions Of 10mentioning

confidence: 99%

Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.)

2013

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-Anthracnose, caused by Colletotrichum gloeosporioides, produces brown lesions on guava fruits, causing severe losses on postharvest. In this study, the infection and colonization of guava fruits by C. gloeosporioides has been examined using scanning and transmission electron microscopy. Fruits at the physiologically mature stage were inoculated with a 10 5 conidia/mL spore suspension. Afterward, fruits were incubated at 25 °C in a wet chamber for periods of 6, 12, 24, 48, 96 and 120 h to allow examination of the infection and colonization process. Conidia germination and appressoria formation occurred six hours after inoculation (h.a.i). Penetration occurred directly via penetration pegs from appressoria, which penetrated the host cuticle 48 h.a.i. Notably, the appressoria did not produce an appressorial cone surrounding the penetration pore. Infection vesicles were found in epidermal cells 96 h.a.i. The same fungal structures were found in epidermal and parenchymal cells of the host 120 h.a.i. Colonization strategy of C. gloeosporioides on guava fruit was intracellular hemibiotrophic. Index terms: colonization, penetration, pre-penetration, ultrastructure. HISTOPATOLOGIA DE Colletotrichum gloeosporioides EM FRUTOS DE GOIABEIRA (Psidium guajava L.).RESUMO -A antracnose, causada por Colletotrichum gloeosporioides, é caracterizada por lesões marrons nos frutos de goiabeira, causando severas perdas em pós-colheita. Os processos de infecção e colonização de C. gloeosporioides foram estudados usando microscópio eletrônico de varredura e transmissão. Frutos em plena maturação fisiológica foram inoculados com suspensão de 10 5 conídios/mL. Posteriormente, os frutos foram incubados em câmara úmida a 25 °C, por períodos de molhamento de 6; 12; 24; 48; 96 e 120 h para o estudo do processo de infecção e colonização. Germinação de conídios e formação de apressórios ocorreram seis horas após a inoculação (h.a.i). A penetração ocorreu diretamente, por meio da formação do peg de penetração emitido da base do apressório, o qual penetrou na cutícula 48 h.a.i. O apressório não produziu o cone apressorial ao redor do poro de penetração. Foram observadas vesículas de infecção nas células epidérmicas 96 h.a.i. As mesmas estruturas fúngicas foram encontradas nas células epidérmicas e parenquimáticas do hospedeiro, após 120 h da inoculação. A estratégia de colonização de C. gloeosporioides foi do tipo hemibiotrófica intracelular. Termos para indexação: Colonização, penetração, pré-penetração, ultraestrutura. JEGER, 1992;JEFFRIES et al., 1990;PRUSKY;FREEMAN;DICKMAN, 2000). Although anthracnose symptoms may appear in the field, depending on the host and environmental conditions, Colletotrichum also cause quiescent infections in fruit, placing them amongst the most important postharvest pathogen. Thereby the pathogen infects the fruit still in the field, but symptoms appear only during ripening (JEFFRIES et al. 1990).Anthracnose is one of the main guava diseases in all guava-growing countries, causing considerable postharvest...

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Section: Suspensions Of 10mentioning

confidence: 99%

Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.)

2013

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“…The acervuli were located in the epidermis, breaking the tissue surface, where the development of conidiophores and arrows could be noted. The formation of acervuli in hypocotyls was previously reported in an article written by Lins, Abreu and Alves (10), showing that conidiophores were formed on a subcuticular stroma, breaking the cuticle after inoculation. Ferreira (4) also observed acervulus formation and conidiophore emergence through the stomata at 96 hours after inoculation of different C. gloeosporioides strains.…”

Section: Resultsmentioning

confidence: 55%

GFP-fluorescent protein on the study of blister spot in coffee plants

et al. 2015

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In Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms.

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“…In seedlings with pairs of true leaves, we observed symptoms of anthracnose ( Figure 1B) and blister spot ( Figure 1D) as well as seedling wilt caused by an intense necrotic area in the stem ( Figure 1E) and apical stem. This wilt may have been caused by the fungus colonizing conducting vessels for sap, and it has been observed by Lins et al (2007) and Ferreira et al (2009). Seedlings derived from plants without blister spot did not show any symptoms of the disease ( Figure 1G and 1H).…”

Section: Treatmentsmentioning

confidence: 83%

“…In Brazil, reports of Colletotrichum complex diseases are associated with anthracnose on branches, fruits and leaves, dieback and blister spot (PARADELA FILHO et al, 2001;OROZCO-MIRANDA, 2003). The etiology of the diseases has been attributed to the species Colletotrichum gloeosporioides, which is found in all organs of coffee plants exhibiting foliar symptoms of the disease (OROZCO-MIRANDA, 2003;LINS et al, 2007;MARQUES, 2009;FERREIRA et al, 2009;PEREIRA et al, 2009). According to Paradela Filho et al (2001), the most critical and damaging injuries from Anthracnose in coffee plants are those in which the fungus localizes on the buds, flowers and small green berries, which die and fall, and causes blackening and death of branches.…”

Section: Introductionmentioning

confidence: 99%

Viability and susceptibility of propagation material from coffee plants to Colletotrichum sp.

et al. 2015

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This study aimed to verify the viability of propagation material from coffee plants descended from germplasm susceptible to blister spot disease as well as its susceptibility to Colletotrichum sp. relative to commercial coffee cultivars. In the first experiment, fruits were harvested from plants with and without symptoms of blister spot and sowed in trays containing a commercial sterilized substrate. The percentages of germinated seeds, viable plantlets and seedlings were evaluated. In diseased tissues, pathogens were isolated and identified though a pathogenicity test. In the second experiment, ten commercial cultivars and one cultivar originating from plants with blister spot were inoculated with the pathogens to assess the severity of anthracnose. Significant differences were not observed with respect to seed germination. However, the viability of plantlets and seedlings was reduced in the cultivar originating from plants with blister spot (Genotype Originated from Diseased Plants-GODP). These plants showed characteristic symptoms of blister spot, including necrosis in the leaves and hypocotyls, wilting and death. In the necrotic lesions, we observed characteristic sporulation of Colletotrichum sp. The cultivar most susceptible to anthracnose in cotyledonary leaves was Catuaí Vermelho (GODP), which presented the highest area under the disease progress curve (AUDPC). In conclusion, the viability of propagation material from coffee plants that had descended from plants with symptoms of blister spot (GODP) was reduced compared with plants from other genotypes, although seed germination was not affected. Moreover, GODP species are more susceptible to Anthracnose on the cotyledonary leaves relative to the other analyzed commercial cultivars. This work is the first to report on different symptoms exhibited by seedlings originating from the seeds of plants with symptoms of blister spot.

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Estudos histopatológicos de Colletotrichum spp. em plântulas de cafeeiro

Cited by 17 publications

References 14 publications

Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.)

Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.)

GFP-fluorescent protein on the study of blister spot in coffee plants

Viability and susceptibility of propagation material from coffee plants to Colletotrichum sp.

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