Annona squamosa, a new host of Ceratocystis fimbriata

Silveira, Silvaldo Felipe da; Harrington, T. C.; Mussi‐Dias, Vicente; Engelbrecht, Christine; Alfenas, Acelino C.; Silva, Carolina R.

doi:10.1590/s0100-41582006000400010

Cited by 29 publications

(29 citation statements)

References 8 publications

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“…Como outros hospedeiros, citamse: cássia (Cassia sp. ), crotalária (Crotalaria juncea L.), mamona (Ricinus communis L.) e pinha (Annona squamosa) (Morgan-Jones, 1967;MacFarlene, 1968;Harrington, 2000;Barnes et al, 2001;Baker at al., 2003;Marin et al, 2003;Engelbrecht e Harrington, 2005;Silveira et al, 2006;Engelbrecht et al, 2007). A despeito desta ampla distribuição e círculo de hospedeiros, a doença ainda não havia sido relatada em cupuaçuzeiro, mesmo em países aonde já ocorre sobre o cacaueiro há bastante tempo.…”

Section: Resultsunclassified

Murcha De Ceratocystis, Nova Doença Do Cupuaçuzeiro

Oliveira¹,

Niella²,

Silva³

et al. 2013

Agrotropica

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Ceratocystis wilt, a new disease of the cupuassu tree, in Brazil. The cupuassu tree (Theobroma grandiflorum), native of the Amazon region, is cultivated mainly in the states of Para, Amazonas, Acre and Bahia, Brazil. In Bahia, it has been planted in the same municipalities were cacao is grown, especially, in the region known as Baixo Sul. During fitossanitary inspections carried out in one area at the Cacao Research Center (CEPEC), in Ilheus, BA, cultivated with cupuassu, trees showing symptoms of wilting, chlorosis and leaf drying, in addition to canker and necrosis of the stem, were observed. Ceratocystis fimbriata was the fungus, predominately, isolated in PDA medium in all the attempts using necrotic tissues obtained from the stem. Histopathological studies, done at the optical microscope, showed the presence of mycelia and chlamydospores of the fungus in the xylem vessels. Three-and 8-month-old cacao and cupuassu seedlings inoculated with the fungus started exhibiting the disease symptoms 20 and 40 days later, respectively. The fungus was re-isolated from inoculated plants in all the attempts. This is the first record of ceratocystis wilt disease in cupuassu tree.

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Section: Resultsunclassified

Murcha De Ceratocystis, Nova Doença Do Cupuaçuzeiro

Oliveira¹,

Niella²,

Silva³

et al. 2013

Agrotropica

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“…Entre elas, peritécios com pescoços longos e com hifas ostiolares (Figura 2a,b); ascósporos com a aparência de chapéu (Figura 2c); endoconídios cilíndricos, unicelulares, hialinos e catenulados produzidos em endoconidióforos, também cilíndricos, longos e hialinos (Figura 2d,e); além de clamidósporos castanhos, globosos a piriformes ocorrendo tanto isoladamente, quanto em cadeias curtas (Figura 2f) (Morgan-Jones, 1967;Hanlin, 1990). Em exames histológicos realizados em materiais naturalou artificialmente infectados, adotando uma metodologia simples utilizada na diagnose da doença em outros hospedeiros (Ferreira et al, 2005), foram observados hifas e clamidósporos do fungo no interior dos vasos do xilema (Figura 2f (Morgan-Jones, 1967;MacFarlene, 1968;Harrington, 2000;Barnes et al, 2001;Baker at al., 2003;Marin et al, 2003;Engelbrecht e Harrington, 2005;Silveira et al, 2006;Engelbrecht et al, 2007). A despeito desta ampla distribuição e círculo de hospedeiros, a doença ainda não havia sido relatada em cupuaçuzeiro, mesmo em países aonde já ocorre sobre o cacaueiro há bastante tempo.…”

Section: Resultsunclassified

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2013

Agrotropica

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“…This Ceratocystis isolate from passionflower plants (one month of age) was subjected to pathogenic characterization by adopting two methods: inoculation with mycelial disks of this fungus, as described by Silveira et al (2006), and deposit of a suspension of 10 8 cylindrical spores on the stem of the plant (Zauza et al, 2004). Evaluation occurred at 30 days post-inoculation.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Ceratocystis fimbriata from passion fruits

et al. 2016

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RESUMO:Frutos de maracujá (Passiflora edulis) foram encontrados com sintomas de podridão no campo, na cidade de Tanhaçu, Bahia. Após isolamento do patógeno associado a essas podridões, o presente trabalho teve como objetivo realizar a caracterização de um isolado de Ceratocystis de maracujá para melhor compreender esse patossistema. A caracterização molecular foi realizada com base no sequenciamento da região ITS-5.8S rDNA. Realizou-se a caracterização patogênica em mudas e frutos de maracujá. A colonização dos frutos de maracujá foi acompanhada com técnicas de microscopia de varredura (MEV). A análise do DNA do isolado de Ceratocystis mostrou que este pertence à espécie Ceratocystis fimbriata. As mudas de maracujá inoculadas apresentaram lesão 30 dias após a inoculação, e não foram observadas murcha nem morte das plantas inoculadas. Nos frutos não foram constatadas diferenças nas lesões causadas por esse fungo entre os cultivares, com médias de lesões de 1,0 a 2,2 cm de diâmetro, aos 7 e 11 dias, respectivamente. Nas análises realizadas em MEV, foram observadas a germinação dos esporos e a penetração do fungo nos frutos no período entre 2 e 6 horas após a inoculação. Doze e 24 horas após a inoculação foi visualizada a colonização do fruto, tanto externa como internamente, e 48 horas após a inoculação se notou o início da degradação da parede externa da casca do fruto. Noventa horas após a inoculação, observou-se a formação de novos peritécios, tanto na parte interna como na parte externa do fruto. Este estudo vem complementar as informações relacionadas à interação desse fungo com frutos de maracujazeiro. PALAVRAS-CHAVE: podridão; espécies; microscopia.ABSTRACT: Passion fruits (Passiflora edulis) were found with symptoms of rot in the field, in the city of Tanhaçu, Bahia. After isolating the pathogen associated with this rot, in the present study we aimed to characterize the Ceratocystis isolate from passion fruit for better understanding this pathosystem. Molecular characterization was done based on the region ITS-5.8S rDNA. Pathogenic characterization was carried out for seedlings and fruits of passionflower. Passion fruit colonization was monitored by means of scanning electron microscopy techniques (SEM). DNA analysis of the Ceratocystis isolate from passionflower pointed out that this species belongs to Ceratocystis fimbriata. The inoculated passionflower seedlings showed injury at 30 days post-inoculation, but no inoculated plant showed wilt or died. Considering fruits, no differences were found for lesions caused by this fungus among cultivars, and lesions had average diameters of 1.0 and 2.2 cm at 7 and 11 days, respectively. The analysis using SEM indicated fungus spore germination and penetration in the fruit between 2 and 6 hours post-inoculation. At 12 and 24 hours post-inoculation, fruit colonization was noted both externally and internally, while fruit wall degradation started at 48 hours post-inoculation. At 90 hours post-inoculation, formation of new perithecia was observed inside and outside th...

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Annona squamosa, a new host of Ceratocystis fimbriata

Cited by 29 publications

References 8 publications

Murcha De Ceratocystis, Nova Doença Do Cupuaçuzeiro

Murcha De Ceratocystis, Nova Doença Do Cupuaçuzeiro

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Characterization of Ceratocystis fimbriata from passion fruits

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