Fenótipo S1 de esterase em Meloidogyne incognita no Brasil

Oliveira, Dagoberto S.; Oliveira, R. D. L.; Gonçalves, Wallace

doi:10.1590/s0100-41582006000200016

Cited by 5 publications

(3 citation statements)

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“…comm.). This population displaying the esterase phenotype S1 (= S2), presented a perineal pattern resembling M. incognita (Oliveira et al, 2006). Other morphological characters using scanning electron microscopy and SCAR markers should be used to characterize this species.…”

Section: Discussionmentioning

confidence: 99%

Reaction of coffee genotypes to different populations of Meloidogyne spp.: detection of a naturally virulent M. exigua population

Muñiz¹,

Campos²,

Moita³

et al. 2009

Trop. plant pathol.

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The reaction of seven genotypes of Coffea arabica to 10 Meloidogyne spp. populations collected mainly from coffee plantations in Brazil and Costa Rica was evaluated under greenhouse conditions. The inoculum consisted of 10,000 eggs per plant. Evaluations were done 8 months after inoculations considering the root fresh weight, gall and egg mass indices, number of eggs per gram of root and reproduction factor (RF). The cultivars Obatã IAC 1669-20, Sarchimor IAC 4361 and Tupi Amarelo IAC 5111 exhibited susceptibility to the four Brazilian M. exigua populations tested. However, cv. Tupi Vermelho IAC 1669-33 revealed resistance (RF value of 0.7) to the M. exigua population from Lavras, Minas Gerais State, Brazil. A population of M. exigua from Bom Jesus de Itabapoana, Rio de Janeiro State, Brazil, was highly virulent on cv. IAPAR 59 (RF= 165.7), bearing resistance gene Mex-1, and was also virulent on genotype Paraíso (H 419-5-4-5-2) (RF=396.2). A Meloidogyne sp. population on coffee from Garça, São Paulo State, Brazil, reproduced at low rates (RF ranging from 0.1 to 3.9) on all genotypes. All tested cultivars were susceptible to M. incognita and M. paranaensis. M. mayaguensis of guava from Paraná State, Brazil, reproduced at low rates in all coffee genotypes; however, another population of coffee, from Costa Rica, was more aggressive and showed RF value that ranged from 0.8 to 12.4. Results of this study point for the first time to the ability of a naturally occurring M. exigua population to overcome the resistance conferred by the Mex-1 gene.

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Section: Discussionmentioning

confidence: 99%

Reaction of coffee genotypes to different populations of Meloidogyne spp.: detection of a naturally virulent M. exigua population

Muñiz¹,

Campos²,

Moita³

et al. 2009

Trop. plant pathol.

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“…A pesar de que los métodos morfológicos poseen sus ventajas en algunos casos, para conseguir una correcta identificación, se vuelve necesario y obligatorio el uso de otras técnicas como la caracterización enzimática y técnicas de PCR basada en la amplificación de genes de interés (Eisenback & Hunt, 2009;Oliveira et al, 2016). La caracterización bioquímica de nematodos es una metodología muy sensible, que requiere únicamente de la presencia de una hembra joven para poder ser realizada (Carneiro et al, 2005;Oliveira, Oliveira & Gonçalves, 2006). Una comparación de esterasas muestra la mayor precisión para poder distinguir las especies de Meloidogyne; sin embargo, cuando los perfiles de esterasa son semejantes, los perfiles de malato deshidrogenasa son más precisos en la identificación (Horrigue-Raouani et al, 2008;Hunt & Handoo, 2009;Rusinque et al, 2018).…”

Section: Los Nematodos Fitoparásitos Del Génerounclassified

“…También se utilizan perfiles de superóxido dismutasa SOD y glutamato-oxaloacetato transaminasa GOT (Karssen & Moens, 2006, Blok & Powers, 2009. La comparación de perfiles electroforéticos de esterasas muestra una gran consistencia para distinguir especies de Meloidogyne spp Oliveira et al, 2006;Steffen et al, 2007); sin embargo, los perfiles de malato deshidrogenasa ayudan a identificar cuando los perfiles de esterasa son similares, como, por ejemplo, con M. incognita y M. hapla. Algunas especies, como M. arenaria, muestran varios perfiles diferentes, aunque esto puede indicar la existencia de especies crípticas (Hunt & Handoo, 2009).…”

Section: Identificación Por Medio De Isoenzimasunclassified

Identification methods for root-knot nematode Meloidogyne

Zambrano

Cedeño²

2022

Manglar

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Las especies del género Meloidogyne, son de distribución mundial y en general inducen la formación de agallas o nódulos en las raíces de una amplia variedad de plantas, debido a esto, es conocido como "nematodo agallador". Las plantas afectadas manifiestan pérdida de turgencia de las hojas, clorosis y marchitamiento, lo que reduce el rendimiento de los cultivos. En el Ecuador han sido registradas varias especies de Meloidogyne, en plantas silvestres y cultivadas. Para el diagnóstico, la identificación de este fitonemátodo inicialmente fue mediante el uso de caracteres morfológicos y posteriormente se incorporó el uso del análisis del patrón de isoenzimas en gel de poliacrilamida. Sin embargo, las técnicas moleculares, que incluyen el análisis de secuencias parciales o totales de uno o más genes de importancia taxonómica, han generado una revolución en la Taxonomía en general, por lo que actualmente es una de las estrategias con enorme potencial tanto para la Identificación como para el estudio genético de Meloidogyne spp., debido a su alta sensibilidad y confiabilidad del resultado. El surgimiento de modernas técnicas de identificación permitirá una correcta identificación de Meloidogyne spp., lo que contribuye al diseño de adecuadas estrategias de manejo de este nematodo fitopatógeno.

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Biometrical, biological, biochemical and molecular characteristics of Meloidogyne incognita isolates and related species

et al. 2012

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Meloidogyne incognita is one of the most polyphagous species of root-knot nematodes occurring in Brazil and worldwide. Eight M. incognita isolates were studied, representing two enzymatic phenotypes (esterase and malate desydrogenase: I1/N1, I2/N1) and four cryptic Meloidogyne sp.1 (S2/N1) isolates, representing one cytological type (3n040-46). Three M. hispanica isolates (Hi3/N1, 2n032-36) and two of an atypical Meloidogyne sp.2 (S2a/N3, 3n040-44) were included in this study for comparison. All isolates were tested with three M. incognita-specific molecular markers. The primer pairs B06F/R, miF/R and incK14F/R amplified three species-specific fragments of 1,200 bp, 955 bp and 399 bp, respectively for M. incognita and Meloidogyne sp.1 isolates. No amplification occurred in the M. hispanica and Meloidogyne sp.2 isolates, except with primers miF/R (1,650 bp). The genetic variability of the Meloidogyne spp. isolates was evaluated, using RAPD and ISSR markers. The phylogenetic analyses revealed two strongly supported monophyletic clades: clade I, consisting of M. hispanica and the atypical Meloidogyne sp.2 isolates, and clade II, clustering together all M. incognita and the Meloidogyne sp.1 isolates. Considering the biometrical, cytological and molecular approaches, it was possible to conclude that the isolates with three enzymatic phenotypes (I1/N1, I2/N1 and S2/N1) presented the characteristics described for M. incognita. Some correlations were detected between the isozymatic phenotypes and the tree topology (S2a/N3, Hi3/N1, I1/N1, S2/N1), but no strict correlation could be observed for the phenotype I2/N1 and one isolate of S2/N1. Morphologically, the Msp.2 isolates differ from M. incognita and M.

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Fenótipo S1 de esterase em Meloidogyne incognita no Brasil

Abstract: Esterase phenotype S1 in Meloidogyne incognita in Brazilian coffee plantationsThe rare esterase phenotype S1 of Meloidogyne incognita is reported here for the first time in Brazil. The root-knot nematode studied population was detected in a coffee (Coffea arabica)

Cited by 5 publications

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Reaction of coffee genotypes to different populations of Meloidogyne spp.: detection of a naturally virulent M. exigua population

Reaction of coffee genotypes to different populations of Meloidogyne spp.: detection of a naturally virulent M. exigua population

Identification methods for root-knot nematode Meloidogyne

Biometrical, biological, biochemical and molecular characteristics of Meloidogyne incognita isolates and related species

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