Caracterização de estirpes de Ralstonia solanacearum isoladas de plantas de batata com murcha bacteriana, por PCR-Rep e RAPD

Silveira, José Ricardo Pfeifer; Duarte, Valmir; Moraes, Marcelo Gravina de; Oliveira, Andréia Mara Rotta de; Barni, Valmor; Maciel, João Leodato Nunes

doi:10.1590/s0100-41582005000600008

Cited by 6 publications

(12 citation statements)

References 15 publications

(23 reference statements)

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“…It is interesting to highlight that, in this review, some studies about the genetic variability of this bacterial population in Brazil report that the existence of diversity among strains oftentimes is correlated with its geographical origin (SILVEIRA et al, 2005;FONSECA et al, 2014), similarly to what was established for the classification into phylotypes. Therefore, the lower local bacterial variability allows disease control through the use of resistant cultivars recommended for each region of the country, although care should be taken against the dissemination of strains via propagation material.…”

Section: Genetic Diversity Of Ralstonia Solanacearum In Brazilmentioning

confidence: 53%

“…Subsequently, its occurrence was reported in potato in South of Brazil, although in lower frequency than R3Bv2 (LOPES; NAZARENO;FURIATTI, 1993;MACIEL et al, 2001;MORAES, 2002;SILVEIRA et al, 2005). Moreover, under special conditions of moisture and temperature, it has caused significant losses in commercial eucalypt nurseries in the States of Espírito Santo, Santa Catarina, Maranhão, Minas Gerais, Bahia, Pará, and Goiás (ALFENAS et al, 2006;AUER;.…”

Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 99%

“…Analysis of genetic diversity can be conducted based on repetitive sequences (rep-PCR), comprised by the elements BOX, ERIC, and REP, by randomly amplified DNA (RAPD), by amplification of restriction fragments (AFLP), repeated simple sequences (SSR) and by polymorphisms based on restriction fragment size (RFLP) (JAUNET;WANG, 1999;VANDEWALLE;LUISETTI, 1999;COENYE;VANDAMME, 2003;YU et al, 2003;KUMAR;SARMA;ANANDARAJ, 2004;SILVEIRA et al, 2005;LOPES, 2007;IVEY et al, 2007). Silveira et al (2005) investigated the genetic variability of R. solanacearum strains obtained from different potato producing areas in Rio Grande do Sul, using RAPD and repetitive sequences, differentiating biovars1 and 2 by ERIC and BOX-PCR. In this case, only BOX-PCR could confirm the variability within strains of R1Bv1.…”

Section: Genetic Diversity Of Ralstonia Solanacearum In Brazilmentioning

confidence: 99%

“…However, it is important to note that the ability of RAPD in detecting polymorphisms depends on the selection of primer oligonucleotides that will reveal greater variability among and within strains of the biovar being studied. While the population of R. solanacearum in Rio Grande do Sul was quite homogeneous (SILVEIRA et al, 2005), bacterial strains from the Amazon region presented high degree of polymorphism by BOX-PCR, with no correlation among genome profiles and source host, biovar, ecosystem or collection location (COSTA; FERREIRA; LOPES, 2007).…”

Section: Genetic Diversity Of Ralstonia Solanacearum In Brazilmentioning

confidence: 99%

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Occurrence and diversity of Ralstonia solanacearum populations in Brazil

et al. 2015

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ABSTRACT:Ralstonia solanacearum is a gram-negative soil-borne bacterium capable of infection of hundreds of vegetable species over more than 50 botanical families, causing bacterial wilt, except for bananas, when the disease is called Moko. It deserves special attention, from all other plant pathogenic bacteria, for its high phenotypic and genotypic plasticity, a characteristic that makes disease control extremely difficult. In this context, frequent and necessary surveys are conduct in the attempt of characterizing the prevailing strains of R. solanacearum in each region where the disease has been reported. However, knowledge about occurrence and diversity of R. solanacearum in Brazil is fragmented and in some cases, based on inconclusive studies with few strains, little representative of a given region. The need to obtain a greater picture guided this review. The occurrence of this bacterium in Brazilian States and the possible causes for its dissemination are presented, with emphasis on studies of genetic variability of populations of R. solanacearum in the country. The compiled results report a wide distribution of the bacterium in Brazil and great variability of its populations among locations. Partly due to the difficulty of detecting small titer of bacteria in samples, paucity of information about the origin of inoculum in certain regions is observed, as well as the need for detecting the presence of the pathogen in asymptomatic plants, potato tubers with latent infections, soil, and water, which are the major causes of bacterial dissemination into areas without any disease history.

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Section: Genetic Diversity Of Ralstonia Solanacearum In Brazilmentioning

confidence: 53%

Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 99%

Section: Genetic Diversity Of Ralstonia Solanacearum In Brazilmentioning

confidence: 99%

Section: Genetic Diversity Of Ralstonia Solanacearum In Brazilmentioning

confidence: 99%

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Occurrence and diversity of Ralstonia solanacearum populations in Brazil

et al. 2015

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“…Diversos autores (Bringel et al, 2004;Silveira et al, 2005;Costa et al, 2007) têm direcionado seus estudos para a caracterização e diversidade de isolados de R. solanacearum em estados brasileiros. Entretanto, todos esses estudos focam a raça 1 do patógeno, não sendo registrado, até o momento, um estudo de caracterização da diversidade de isolados de R. solanacearum raça 2 coletados em regiões brasileiras.…”

Section: Introductionunclassified

Diversidade genética de isolados de Ralstonia solanacearum e caracterização molecular quanto a filotipos e sequevares

Pinheiro

Amorim

Diniz

et al. 2011

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi identificar isolados brasileiros de Ralstonia solanacearum quanto a filotipos e sequevares, determinar sua diversidade genética, realizar a associação da estrutura genética do patógeno com sua classificação e origem geográfica e identificar um marcador molecular para a diagnose do moko-da-bananeira. Um grupo de 33 isolados de R. solanacearum, da coleção da Embrapa Tabuleiros Costeiros, coletado de diversos hospedeiros, foi caracterizado por meio de PCR em sequência palindrômica extragênica repetitiva (rep-PCR) e RAPD. Deste grupo, 19 perteciam à raça 2 do patógeno e 14 à raça 1, e 15 isolados eram associados à cultura da bananeira. Os filotipos e sequevares foram caracterizados e determinados por PCR Multiplex. Verificou-se que 82% dos isolados pertencem ao filotipo II, e 12% ao filotipo III. Todos os isolados da bananeira pertencem ao filotipo II. A técnica de RAPD foi eficiente em agrupar os isolados de acordo com sua origem geográfica; entretanto, ela requer um número elevado de marcas moleculares. Foi possível relacionar os isolados pela análise rep-PCR. O iniciador com sequências repetitivas enterobacterianas intergênicas de consenso (ERIC) possibilitou a separação dos isolados de acordo com a raça, e o iniciador REP permitiu a discriminação entre os filotipos. Estas duas análises foram as mais informativas.Termos para indexação: Musa, marcador molecular, moko-da-bananeira, murcha bacteriana, rep-PCR, variabilidade genética. Genetic diversity of Ralstonia solanacearum isolates and molecular characterization as to phylotypes and sequevarsAbstract -The objective of this work was to identify Brazilian isolates of Ralstonia solanacearum according to phylotypes and sequevars, to determine their genetic diversity, to associate the pathogen genetic structure with its taxonomy and geographical origin, and to identify a specific molecular marker to diagnose banana moko disease. A group of 33 isolates of R. solanacearum, from the collection of Embrapa Tabuleiros Costeiros, collected from different plant hosts, was characterized using the repetitive extragenic palindromic sequence-based PCR (rep-PCR) and RAPD. From this group, 19 belonged to the pathogen race 2 and 14 to the race 1, and 15 isolates were associated with banana crop. Phylotypes and sequevars were characterized and determined by Multiplex PCR. It was verified that the isolates belonged to phylotypes II (82%) and III (12%). All isolates from banana plants belonged to phylotype II. The RAPD technique was efficient in grouping these isolates according to their geographical origin; however, it requires a large number of molecular markers. It was possible to establish the relationships among the isolates by rep-PCR. The enterobacterial repetitive intergenic consensus primer (ERIC) made it possible to separate the isolates according to the race, and the REP primer allowed for the discrimination among phylotypes. These were the two most informative analyses.

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Genetic Structure of Ralstonia solanacearum and Ralstonia pseudosolanacearum in Brazil

et al. 2020

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Bacterial wilt-causing Ralstonia threaten numerous crops throughout the world. We studied the population structure of 196 isolates of Ralstonia solanacearum and 39 isolates of Ralstonia pseudosolanacearum, which were collected from potato- and tomato-growing areas in 19 states of Brazil. Regardless of the species, three groups of isolates were identified. One group encompassed R. pseudosolanacearum isolates. The other two groups comprise isolates of R. solanacearum (phylotype II) split according to geographic regions, one made of isolates from the North and Northeast and the other made of isolates from the Central, Southeast, and South regions (CSS). Among the isolates collected in CSS, those from tomato were genetically distinct from the potato isolates. The genetic variability in the population of R. pseudosolanacearum was lower than that of R. solanacearum, suggesting that the former was introduced in Brazil. Conversely, the high genetic variability of R. solanacearum in all regions, hosts, and times supports the hypothesis that this species is autochthonous in South America, more precisely in Brazil and Peru. For R. solanacearum, higher variability and lower migration rates were observed when tomato isolates were analyzed, indicating that the variability is caused mainly by the differences of the local, native soil population. The North subpopulation was distinct from all others, possibly because of differences in environmental features of this region. The proximity of some geographic regions and the movement of potato tubers could have facilitated migration and therefore low genetic differentiation between geographic regions. Finally, geography, which also influences host distribution, affects the structure of the population of R. solanacearum in Brazil. Despite quarantine procedures in Brazil, increasing levels of trade are a threat to biosecurity, and these results emphasize the need for improving our regional efforts to prevent the dispersal of pathogens.

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Caracterização de estirpes de Ralstonia solanacearum isoladas de plantas de batata com murcha bacteriana, por PCR-Rep e RAPD

Cited by 6 publications

References 15 publications

Occurrence and diversity of Ralstonia solanacearum populations in Brazil

Occurrence and diversity of Ralstonia solanacearum populations in Brazil

Diversidade genética de isolados de Ralstonia solanacearum e caracterização molecular quanto a filotipos e sequevares

Genetic Structure of Ralstonia solanacearum and Ralstonia pseudosolanacearum in Brazil

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