Incidência de Fusarium solani em mandioca no estado do Pará

Poltronieri, L. S.; Trindade, D. R.; Albuquerque, F. C. de; Duarte, M. L. R.; Cardoso, S. S.

doi:10.1590/s0100-41582002000500019

Cited by 3 publications

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“…Este alto índice de incidência da podridão radicular em mudas de mandioca havia sido relatado, em condições casa-de-vegetação, por Poltronieri et al (2002), utilizando a cultivar Olho de Boto.…”

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Esporulação e crescimento micelial de Fusarium solani em diferentes meios de cultura e regimes de luminosidade

Silva¹,

Teixeira²

2012

AGRO@MBIENTE

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Resumo -Visando a caracterização fisiológica de Fusarium solani isolado de raízes de mandioca, objetivou-se com este trabalho avaliar a esporulação e o crescimento micelial de F. solani em diferentes meios de cultura e regimes de luminosidade. O fungo foi cultivado utilizando cinco meios de cultura (batata dextrose ágar, batata sacarose ágar, mandioca ágar, micophil e ágar-água) sob três regimes de luminosidade (escuro contínuo, fotoperíodo de 12 h e luz contínua) durante o período de incubação de sete dias, a temperatura de 25 °C ± 2 o C. O ensaio foi conduzido em delineamento inteiramente casualizado, em esquema fatorial (5x3), com três repetições. Discos de 5 mm de diâmetro, retirados da borda da colônia cultivada em meio BDA, foram transferidos para o centro de placas de Petri contendo 20 mL de cada meio. Determinou-se o crescimento micelial por meio da medida do diâmetro das colônias em dois sentidos diametralmente opostos, enquanto a esporulação por meio da quantificação de conídios pelo método da gota. Foram observadas variações significativas na produção de massa micelial e conídios nos diferentes meios de cultura e regimes de luminosidade testados, sendo que BDA e BSA sob regime de luz contínua induziram maior crescimento micelial e produção de conídios. Enquanto que no meio AA sob escuro contínuo ocorreu às menores taxas de esporulação e crescimento micelial. Palavras-chaves -Avaliação. Fisiologia. Manihot esculenta. Podridão radicular.Abstract -Considering the physiological characterization of Fusarium solani isolated from cassava roots, the objective of this study was to evaluate mycelial growth and sporulation of F. solani in different culture media and lighting regimes. The fungus was grown using five culture media (potato dextrose agar, potato sucrose agar, cassava, agar-agar, and water micophil) under three light regimes (continuous darkness, a photoperiod of 12 h, and continuous light) during the incubation period of seven day, temperature 25 °C ± 2 °C. The trial was done in completely randomized design with three replications. Discs of 5mm diameter taken from the edge of the colony grown on PDA medium were transferred to the center of Petri dishes containing 20 mL of each medium. Mycelial growth was determined by measuring the diameter of the colonies in two diametrically opposite directions while sporulation by quantifying conidia by drop method. No significant changes in the production of conidia and mycelial mass in different culture media and lighting regimes tested, and BDA and BSA under the regime of continuous light best sporulation and conidial production were observed. While in the midst AA under continuous darkness was the lowest rates of mycelial growth and sporulation.

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Section: Resultsunclassified

Esporulação e crescimento micelial de Fusarium solani em diferentes meios de cultura e regimes de luminosidade

Silva¹,

Teixeira²

2012

AGRO@MBIENTE

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Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers

Brasileiro¹,

Coimbra²,

Morais³

et al. 2004

Braz. J. Microbiol.

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Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates. Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical origin and substrate. Its polymorphism was observed even in the very conserved sequence of rDNA locus, and the SPAR marker (GTG)5 showed the highest polymorphism. Together, those results may contribute to understand the relation between fungal genetic variability and cultivars resistance phenotypes to fungal-caused diseases, helping plant-breeding programs.

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Intracellular Siderophore Detection in an Egyptian, Cobalt-Treated F. solani Isolate Using SEM-EDX with Reference to its Tolerance

Rasha

2017

Polish Journal of Microbiology

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A b s t r a c tAn Egyptian, plant pathogenic Fusarium solani isolate was grown on cobalt concentrations of 0, 50, 200, 500, 800, and 1000 ppm. The isolate survived concentrations up to 800 ppm, however failed to grow at 1000 ppm. Morphology and elemental analysis of the isolate under the investigated Co concentrations were studied using Scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). The isolate reserved its morphology up to a concentration of 200 ppm. Morphological distortions were dramatic at 500 and 800 ppm. EDX detected Co uptake through the hyphae, microconidia, macroconidia, and chlamydospores. Iron, calcium, and potassium were also detected. EDX results showed a linear relationship between Co% and Fe% up to a concentration of 500 ppm reflecting the possible ability of the isolate to synthesize intracellular siderophores storing iron and their release from the vacuoles. The participation of such siderophores in conferring tolerance against cobalt is discussed. At 800 ppm, the % of Fe was greatly reduced with an accompanying increase in morphological distortions and absence of microconidia. Increasing the implicated cobalt concentrations resulted in increasing the percentages of the chelated cobalt reflecting the possible implication of the cell wall as well as extracellular siderophores in the uptake of cobalt. The current results recommend the absence of cobalt in any control regime taken to combat the investigated F. solani isolate and highlights the accomplishment of biochemical, ultrastructural, and molecular studies on such isolate to approve the production of siderophores and the role of cell wall in cobalt uptake.K e y w o r d s: Fusarium solani, cobalt uptake, cobalt tolerance, siderophores

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Incidência de Fusarium solani em mandioca no estado do Pará

Cited by 3 publications

References 0 publications

Esporulação e crescimento micelial de Fusarium solani em diferentes meios de cultura e regimes de luminosidade

Esporulação e crescimento micelial de Fusarium solani em diferentes meios de cultura e regimes de luminosidade

Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers

Intracellular Siderophore Detection in an Egyptian, Cobalt-Treated F. solani Isolate Using SEM-EDX with Reference to its Tolerance

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