Seed-tuber transmission of Spongospora subterranea

Nazareno, Nilceu Ricetti Xavier de; Boschetto, Norma

doi:10.1590/s0100-41582002000200019

Cited by 3 publications

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“…A survey in the main potato-producing zones of Pakistan showed that contaminated soils were found in most of the locations examined (Iftikhar et al 2003). The rising incidence of powdery scab in Brazil is of great concern as the disease was thought to be restricted to the centre-pivoted irrigation systems around São Paulo (de Nazareno and Boschetto 2002). Similarly, more widespread distribution of powdery scab than previously recognized was recently found in Argentina (Clausen et al 2005).…”

Section: Importance Occurrence Damagementioning

confidence: 97%

Review: Powdery Scab of Potato—Increased Knowledge of Pathogen Biology and Disease Epidemiology for Effective Disease Management

Merz

Falloon

2008

Potato Res.

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Section: Importance Occurrence Damagementioning

confidence: 97%

Review: Powdery Scab of Potato—Increased Knowledge of Pathogen Biology and Disease Epidemiology for Effective Disease Management

Merz

Falloon

2008

Potato Res.

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“…Other studies reported lower incidence and severity of disease on progeny tubers than on the seed stock from which they were grown (Braithwaite et al. , 1994; de Nazareno & Boschetto, 2002). Furthermore, a number of studies have shown that disease may not develop where temperature and/or soil moisture conditions are unfavourable, even where soils are known to be infested or diseased tubers are planted (Hughes, 1980; Christ & Weidner, 1988; Nakayama et al.…”

Section: Introductionmentioning

confidence: 92%

“…, 2006) or infected tubers in uninfested soil (Falloon et al. , 1996; de Nazareno & Boschetto, 2002) can lead to the development of disease in progeny tubers. In uninfested soils, transmission of the pathogen from infected seed tubers to progeny does not appear to be straightforward.…”

Section: Introductionmentioning

confidence: 99%

Relationship between Spongospora subterranea f. sp. subterranea soil inoculum level, host resistance and powdery scab on potato tubers in the field

Brierley

Sullivan

Wale³

et al. 2012

Plant Pathology

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The relationship between initial soil inoculum level of Spongospora subterranea f. sp. subterranea (Sss) and the incidence and severity of powdery scab on potato tubers at harvest was investigated. In all experiments soil inoculum level of Sss (sporeballs ⁄ g soil) was measured using a quantitative real-time PCR assay. Of 113 commercial potato fields across the UK, soil inoculum was detected in 75%, ranging from 0 to 148 Sss sporeballs ⁄ g soil. When arbitrary soil inoculum threshold values of 0, <10 and >10 sporeballs ⁄ g soil were set, it was observed that the number of progeny crops developing powdery scab increased with the level of inoculum quantified in the field soil preplanting. In four field trials carried out to investigate the link between the amount of inoculum added to the soil and disease development, disease incidence and severity on progeny tubers was found to be significantly (P < 0AE01) greater in plots with increasing levels of inoculum incorporated. There was a cultivar effect in all years, with disease incidence and severity scores being significantly greater in cvs Agria and Estima than in Nicola (P < 0AE01).

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Detection and Quantification of Spongospora subterranea Sporosori in Soil by Quantitative Real-Time PCR

et al. 2019

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Powdery scab on potato tubers is caused by the obligate soilborne biotroph Spongospora subterranea and is known to cause substantial losses in potato production. The pathogen also infects roots of susceptible hosts, forming galls which can negatively affect root function. S. subterranea is also the vector of Potato mop-top virus, which causes a tuber necrosis disease that can, depending on temperature and cultivar, render potato tubers unmarketable. In this study, we adapted a published protocol to develop a sensitive and robust quantitative real-time PCR (qPCR) assay using specific primers and probes for detecting and quantifying S. subterranea sporosori in soil types that differ in physical properties, including organic matter content and soil pH. For the first time, an external control was utilized and applied directly to the soil prior to DNA extraction, which facilitated normalization of S. subterranea sporosori soil levels from sample to sample. The duplex qPCR protocol was demonstrated to be highly sensitive, capable of detecting and quantifying as few as 1 sporosorus/g of soil, with consistently high qPCR efficiency and the coefficient of determination (R2) values ranging from 94 to 99% and 0.98 to 0.99, respectively. The protocol was successfully implemented in enumerating S. subterranea sporosori in naturally infested field soil collected from several states and in artificial potting mixes with high organic matter content ranging from 64 to 71%. The qPCR method developed can be useful for potato growers to avoid agricultural soils highly infested with S. subterranea and in the development of risk assessment models in the future that incorporate cultivar susceptibility to powdery scab and soil infestation levels.

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Seed-tuber transmission of Spongospora subterranea

Abstract: Autor para correspondência: Nilceu R. X. de Nazareno RESUMO Transmissão através de tubérculo semente de Spongospora subterranea * Bolsista do CNPq.

Cited by 3 publications

References 0 publications

Review: Powdery Scab of Potato—Increased Knowledge of Pathogen Biology and Disease Epidemiology for Effective Disease Management

Review: Powdery Scab of Potato—Increased Knowledge of Pathogen Biology and Disease Epidemiology for Effective Disease Management

Relationship between Spongospora subterranea f. sp. subterranea soil inoculum level, host resistance and powdery scab on potato tubers in the field

Detection and Quantification of Spongospora subterranea Sporosori in Soil by Quantitative Real-Time PCR

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