Implantação e implementação das normas das Boas Práticas Laboratoriais (BPL) no laboratório de análises de resíduos da Universidade Estadual de Campinas

Rodrigues, Nádia Regina; Souza, Ana Paula Ferreira de; Watanabe, Marcelo

doi:10.1590/s0100-40422012000600038

Cited by 4 publications

(1 citation statement)

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“…Method Validation: The in house validation was performed according to the criteria and recommendations by SANTÉ 11945/2015 28 and on good laboratory practice (GLP), supported in good scientific practice [29][30] . The parameters considered were: linearity, LOQ, specificity, recovery and precision (repeatability and within-laboratory) and matrix effect.…”

Section: Instrumentation and Conditionsmentioning

confidence: 99%

Analysis of aflatoxin B1 in Brazil nuts: method optimization and validation

2018

JCBSC

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A method for the determination of aflatoxin B1 was validated according to criteria by SANTÉ 11945/2015. The samples were extracted with acetonitrile and the cleaning step was not performed. Quantification was made by liquid chromatography with fluorescence detector and pre-column derivatization with trifluoroacetic acid. No matrix effect was observed and linearity was obtained in the range of 5-80 µg L-1. The recoveries ranged from 73% to 85% with a coefficient of variation of less than 5%. The quantification limit was 6 µg kg-1 and the measurement uncertainty was ± 0.18 µg kg-1 for this level. This method was applied to 14 real samples purchased in the local market and contamination was found in one sample above the limit tolerated by the legislation (59 µg kg-1). It is a low cost method with good sensitivity and easy to execute.

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Section: Instrumentation and Conditionsmentioning

confidence: 99%

Analysis of aflatoxin B1 in Brazil nuts: method optimization and validation

2018

JCBSC

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Occurrence of glyphosate and AMPA residues in soy-based infant formula sold in Brazil

Rodrigues

Souza

2018

Food Additives & Contaminants: Part A

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Glyphosate is an herbicide widely used in the world, being applied in several crops, among them soybeans. Recently, glyphosate and its metabolite aminomethylphosphonic acid (AMPA) have been identified as possible contributors to the emergence of various diseases such as autism, Parkinson's and Alzheimer's diseases, as well as cancer. The child population-consuming cereal-based foods is the most exposed to the effects of pesticides because of their developmental phase and they have a higher food intake per kilogram of body weight than adults. The presence of glyphosate and AMPA residues in soy-based infant formulas was evaluated during the years 2012-2017, totalising 105 analyses carried out on 10 commercial brands from different batches. Glyphosate and AMPA were determined by liquid chromatography with fluorescence detection after derivatisation reaction. The method was validated and showed accuracy and precision with a limit of quantification (LOQ) of 0.02 mg kg. Among those samples that contained levels above the LOQ, the variation of glyphosate residues was from 0.03 mg kg to 1.08 mg kg and for AMPA residues was from 0.02 mg kg to 0.17 mg kg. This is the first scientific communication about glyphosate and AMPA contamination in soy-based infant formula in Brazil, The study was conducted under good laboratory practice (GLP) and supported by good scientific practice.

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