Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules

Abstract

Recebido em 1/6/11; aceito em 2/9/11; publicado na web em 7/10/11The bioassay, first order derivative UV spectrophotometry and chromatographic methods for assaying fluconazole capsules were compared. They have shown great advantages over the earlier published methods. Using the first order derivative, the UV spectrophotometry method does not suffer interference of excipients. Validation parameters such as linearity, precision, accuracy, limit of detection and limit of quantitation were determined. All methods were linear and reliable within acceptable limits for antibiotic pharmaceutical preparations being accurate, precise and reproducible. The application of each method as a routine analysis should be investigated considering cost, simplicity, equipment, solvents, speed, and application to large or small workloads.

Biowaiver Monograph for Immediate-Release Solid Oral Dosage Forms: Fluconazole

“…56 Other reports indicate a log P value of approximately 0.5, 17, 25,57,58 which would correspond to a moderate lipophilicity. It contains two weakly basic ionization centers that are triazolyl centers with a pKa value of 2.03 at 37 • C. 59,60 Three different pKa values of 11.01 ± 0.29, 2.94 ± 0.10, and Table 3 shows IR fluconazole dosage formulations with marketing authorization (MAs) in various countries. These MAs cover a range of strengths corresponding to 50, 150, and 200 mg fluconazole.…”

Development and Validation of First Derivative Spectrophotometric Method for Quantification of Darunavir in Tablets

“…figure 2, it is an intense, better characterized valley. The increased selectivity observed in the derivative spectroscopy is based on the relationship between the amplitude of the derivative absorbance and the wavelength which is inversely proportional to the bandwidth of the ordinary spectrum [16].…”

Comparative study of normal, micro- & nano-sized iron oxide effect in potentiometric determination of fluconazole in biological fluids

“…4 However, some of these methods need expensive equipment and/ or are time-consuming. Moreover, several spectroscopic methods have been developed for the determination of FLU, [5][6][7][8][9] but they need pretreatment steps before application in biological uids.…”