A validated method using RP-HPLC for quantification of reserpine in the Brazilian tree Rauvolfia sellowii Müll. Arg. (Apocynaceae)

Baratto, Leopoldo Clemente; Campos, Francinete Ramos; Pontarolo, Roberto; Santos, Cid Aimbiré de Moraes

doi:10.1590/s0100-40422012000200031

Cited by 5 publications

(3 citation statements)

References 18 publications

(16 reference statements)

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“…They observed that the reserpine content was ranged from 0.0382 to 0.1442%. Baratto et al (2012) quantified reserpine content in the dried stem bark of R. sellowii by HPLC and found 0.01% dry weight basis.…”

Section: Resultsmentioning

confidence: 99%

Quantification of reserpine content and antibacterial activity of Rauvolfia serpentina (L.) Benth. ex Kurz

Negi¹,

Bisht²,

Bhandari³

et al. 2014

Afr. J. Microbiol. Res.

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Reserpine is well known bioactive compound isolated from Rauvolfia serpentina. The aim of this study was to quantify reserpine content and evaluate the antibacterial activity of methanol extracts of R. serpentina against Salmonella typhimurium, Escherichia coli, Citrobacter freundii, Proteus vulgaris, Enterococcus faecalis and Staphylococcus aureus. Roots of R. serpentina were collected from Gadarpur and Uttarakhashi of Uttarakhand State, India. The antibacterial activity of the methanol extracts was evaluated by determination of minimum inhibitory concentration (MIC) and the diameter of zone of inhibition (ZOI) against both Gram positive and Gram negative bacteria using agar well diffusion method. The study reveals that reserpine content was higher (0.37%) in the sample collected from Gadarpur, whereas it was found to be 0.31% in sample collected from Uttarakashi. The highest zone of inhibition (13 mm) with lowest MIC (625 µg) was observed against Staphylococcus aureus and highest MIC (10 mg) was observed against Escherichia coli, whereas Proteus vulgaris was observed resistant to tested extracts upto 10 mg. R. serpentina contain good amount of reserpine and exhibited strong antibacterial activity against most of the tested human pathogenic bacteria. Therefore, the results of the study support the folklore claim of the plant species.

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Section: Resultsmentioning

confidence: 99%

Quantification of reserpine content and antibacterial activity of Rauvolfia serpentina (L.) Benth. ex Kurz

Negi¹,

Bisht²,

Bhandari³

et al. 2014

Afr. J. Microbiol. Res.

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“…High-performance liquid chromatography (HPLC) has been successfully applied to analyze plant extracts and products. [ 13 14 ] This method has several advantages, such as efficient separation, identification and quantification of similar compounds. However, to the best of our knowledge, no HPLC-UV simultaneous method has been reported to evaluate extracts and products from S. terebinthifolius .…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a simultaneous RP-HPLC-UV/DAD method for determination of polyphenols in gels containing S. terebinthifolius raddi (Anacardiaceae)

2017

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Topical gels containing extracts of Schinus terebinthifolius have been used to treat bacterial vaginosis. It has been reported that this species has antimicrobial, anti-inflammatory and anti-ulcerogenic properties, which can be attributed to the presence of phenolic compounds. In this work, a sensitive and selective reversed-phase HPLC-UV/DAD method for the simultaneous assay of six polyphenols that could be present in S. terebinthifolius was developed. The method was shown to be accurate and precise. Peak purity and similarity index both exceeded 0.99. Calibration curves were linear over the concentration range studied, with correlation coefficients between 0.9931 and 0.9974. This method was used to determine the polyphenol content of a hydroalcoholic extract and pharmacy-compounded vaginal gel. Although the method is useful to assess the 6 phenolic compounds, some compounds could not be detected in the products.SUMMARY A sensitive, selective, accurate and precise reversed-phase HPLC-UV/DAD method for the simultaneous assay of six polyphenols in S. terebinthifolius Raddi Abbreviations used: RP-HPLC-UV/DAD: Reverse Phase High Performance Liquid Chromatograph with Ultraviolet and Diode Array Detector, HPLC: High Performance Liquid Chromatograph, HPLC-UV: High Performance Liquid Chromatograph with Ultraviolet Detector, ANVISA: Brazilian National Health Surveillance Agency, LOD: Limit of detection, LOQ: Limit of quantitation

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“…Multiple herbs in polyherbal and herbo-mineral formulations in a meticulous ratio will give an improved therapeutic effect and lessening the toxicity. [4,5] Several chromatographic methods, for example, UV-visible Spectrophotometric, [4][5][6] HPLC, [7][8][9][10] HPTLC, [11][12][13][14][15][16] and methods are established for the qualitative and quantitative estimation of the Ascorbic acid, Gallic acid, Quercetin, and Reserpine separately or together with other chemical markers. This study primarily reports RP-HPLC method for the straightforward and rapid determination of the Ascorbic acid, Gallic acid, Quercetin, and Reserpine in Polyherbal Formulation.…”

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confidence: 99%

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2021

AJP

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Aim:The study aims to develop and validate accurate, precise, and robust reverse phase high performance liquid chromatographic method for determination of phytoconstituents such as ascorbic acid, gallic acid, quercetin, and reserpine in polyherbal formulation and crude drugs. Materials and Methods: The adequate chromatographic separation was accomplished on C 18 column, 250 × 4.6 mm, 5 µmAgilent eclipse using mobile phase 0.05 M sodium dihydrogen phosphate buffer (pH-4 adjusted with 1% Orthophosphoric acid): Acetonitrile with properly resolved gradient program. The flow rate was 1 ml/min, and the ultraviolet detection was monitored at 227 nm. The retention time of ascorbic acid, gallic acid quercetin and reserpine was found to be 3.44 min, 5.26 min, 10.02 min and 13.24 min, respectively. The method was validated as a final verification of method development concerning precision, linearity, accuracy, sensitivity and robustness as per International Council for Harmonization (ICH) guideline Q2 (R1). Results and Discussion: A method is considered to be linear as the correlation coefficient was found to be within acceptance criteria. The detector response was linear in the range of 50-250 µg/ml Ascorbic acid, 100-300 µg/ml Gallic acid, 100-300 µg/ml, Quercetin and 50-250 µg/ml Reserpine. The % RSD of peak area response due to Ascorbic acid, Quercetin, Reserpine and Gallic acid in five replicate injections of standard solution was to be less than 2.0%, and system suitability parameters were passed. Conclusion: The proposed method was effectively applied for the simultaneous estimation of Ascorbic acid, Gallic acid, Quercetin and Reserpine in Polyherbal formulation and in crude drugs.

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A validated method using RP-HPLC for quantification of reserpine in the Brazilian tree Rauvolfia sellowii Müll. Arg. (Apocynaceae)

Cited by 5 publications

References 18 publications

Quantification of reserpine content and antibacterial activity of Rauvolfia serpentina (L.) Benth. ex Kurz

Quantification of reserpine content and antibacterial activity of Rauvolfia serpentina (L.) Benth. ex Kurz

Development and validation of a simultaneous RP-HPLC-UV/DAD method for determination of polyphenols in gels containing S. terebinthifolius raddi (Anacardiaceae)

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