Extraction and isolation of dictamnine, obacunone and fraxinellone from Dictamnus dasycarpus Turcz. by supercritical fluid extraction and high-speed counter-current chromatography

Abstract

Recebido em 19/4/11; aceito em 10/8/11; publicado na web em 30/9/11 Supercritical fluid extraction was used to extract active compounds from the Chinese traditional medicinal D. dasycarpus under the pressure of 30 MPa and temperature of 45 ºC. Further separation and purification was established by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.8:1.3:0.9, volume ratio). The separation yielded a total of 47 mg of dictamnine, 24 mg of obacunone and 83 mg of fraxinellone from 1.0 g of the crude extract in one step separation with the purity of 99.2, 98.4 and 99.0%, respectively, as determined by HPLC. The chemical structures of these compounds were identified by ESI-MS, IR, 1 H-NMR and 13 C-NMR.

Preparative separation and purification of bufadienolides from ChanSu by high-speed counter-current chromatography combined with preparative HPLC

“…HSCCC has been successfully applied to prepare various types of natural products. [8][9][10][11] Ma and co-researchers have isolated several bufadienolides from ChanSu using HSCCC. However, the separation process lasted more than 11 h. 12 Hence, it is necessary to explore a more powerful and efficient method of separating bufadienolides at large quantities in a shorter time.…”

Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte

“…Dictamnus dasycarpus is widely distributed in Asia and has been shown to possess anti-atopic [14], antioxidant [15], anti-inflammatory [15], and anti-allergic [14] properties. Furthermore, D. dasycarpus contains several bioactive alkaloids, limonoids, and terpenoids [19,20,27], and its extract has been reported to alleviate oxazolone-induced atopic-like dermatitis in mice [14]. The present study was performed to identify biologically active components in D. dasycarpus with anti-inflammatory, antioxidant, and/or anti-AD effects by performing in vitro and in vivo experiments in TNF-α/IFN-γ-induced HaCaT cells, H 2 O 2 -treated Jurkat T cells, and in a murine DNCB-induced model of AD.…”

“…The present study was performed to identify biologically active components in D. dasycarpus with anti-inflammatory, antioxidant, and/or anti-AD effects by performing in vitro and in vivo experiments in TNF-α/IFN-γ-induced HaCaT cells, H 2 O 2 -treated Jurkat T cells, and in a murine DNCB-induced model of AD. Fraxinellone, dictamine, and obacunone have been shown to be produced by D. dasycarpus [19,20,27], and haplopine isolated from this plant has been reported to have strong anti-inflammatory and anti-fungal properties [18,20,21]. Therefore, we selected these four compounds and investigated their potentials with respect to F.…”

“…Its root bark, called "Baekseonpi" in Korean, has been used to treat mild skin diseases in traditional medicine in Korea, China, and Japan [13]. D. dasycarpus extracts have been reported to have anti-atopic [14], anti-inflammatory [15], and antioxidant [15], anti-allergic [14] effects, and alkaloids (e.g., dictamine and haplopine) and limonoids (e.g., fraxinellone and obacunone) isolated from D. dasycarpus have been shown to possess anti-inflammatory [16][17][18], antioxidative [18][19][20], and antifungal [21] activities. Although many studies have been conducted on the bioactivity of D. dasycarpus, few have investigated the use of its constituent compounds for the treatment of AD.…”

Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

“…HSCCC has been successfully applied to the isolation and purification of compounds from natural materials. [10][11][12][13] However, so far no reports have been published on the purification of artichoke components by HSCCC.…”