Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte

Kim, Tae‐Young; Kim, Ye Jin; Jegal, Jonghwan; Jo, Beom-Geun; Choi, Han‐Seok; Yang, Min

doi:10.3390/antiox10050806

Cited by 16 publications

(12 citation statements)

References 47 publications

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“…More related antioxidants are still being studied. For example, it has recently been found that Haplopine, a biologically active component of plants, increases the expression of SOD, CAT, HO-1 in a concentration-dependent manner in vitro and has excellent therapeutic potential in AD mouse models ( Kim et al, 2021 ). Many inorganic substances have strong antioxidant properties, such as the previously mentioned H 2 S (one of the RSS).…”

Section: Novel Therapeutic Approaches For Inflammatory Skin Diseasesmentioning

confidence: 99%

Oxidative Stress and Gut Microbiome in Inflammatory Skin Diseases

Zhang

et al. 2022

Front. Cell Dev. Biol.

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Oxidative stress plays a dominant role in inflammatory skin diseases. Emerging evidence has shown that the close interaction occurred between oxidative stress and the gut microbiome. Overall, in this review, we have summarized the impact of oxidative stress and gut microbiome during the progression and treatment for inflammatory skin diseases, the interactions between gut dysbiosis and redox imbalance, and discussed the potential possible role of oxidative stress in the gut-skin axis. In addition, we have also elucidated the promising gut microbiome/redox-targeted therapeutic strategies for inflammatory skin diseases.

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Section: Novel Therapeutic Approaches For Inflammatory Skin Diseasesmentioning

confidence: 99%

Oxidative Stress and Gut Microbiome in Inflammatory Skin Diseases

Zhang

et al. 2022

Front. Cell Dev. Biol.

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“…The expression of HO-1 was increased when the skin is exposed to environmental stimuli such as H 2 O 2 induced oxidative stress or in ammatory factors to give play to cytoprotective effects of anti-in ammatory [12], antioxidant [13], anti-apoptotic [14] and maintenance of epithelial cell integrity [15]. HO-1 attenuates the development of atopic dermatitis-like lesions in mice [16][17][18], suggesting HO-1 may play a certain role in the development of AD, but the mechanisms of its action involved are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: HO-1 may be the potential therapeutic target for Atopic dermatitis as oxidative stress is dysregulated and associated with inflammation in Atopic dermatitis patients

Yuan

Wang

et al. 2022

Preprint

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Atopic dermatitis (AD) is a chronic inflammatory skin disease and oxidative stress plays pathogenetic role in it. Heme oxygenase-1 (HO-1) is a key enzyme in response to oxidative and inflammatory insults. This study aims to evaluate the correlation between HO-1 expression, oxidative stress and inflammation in AD patients, identify the antioxidative and anti-inflammation effects of HO-1 on H2O2-induced HaCaT cells. Forty-two AD patients and twelve healthy controls were enrolled. Human Oxidative Stress PCR Array was performed to test the oxidative stress related gene expression in AD patients and controls. The HO-1 expression, malondialdehyde (MDA) and SOD and inflammatory factors IL-4 and high mobility group box1 (HMGB1) were measured by ELISA. The effect of HO-1 on the expressions of ROS, MDA and SOD, and inflammatory factors IL-4 and TNF-α were also detected in H2O2-induced HaCaT cells. We demonstrated that the levels of MDA were increased while SOD, antioxidants genes, ROS metabolisms and oxygen transporter genes were decreased in AD patients than in controls. In addition, high expression of MDA is positively correlated with IL-4 expression and disease severity of AD. HMGB1was also positively correlated with SOD activity. Furthermore, the level of HO-1 was significantly higher in AD patients than controls and HO-1 increased the SOD activity and decreased the levels of ROS, MDA, decreased the expressions of IL-4 and TNF-α in H2O2 -induced oxidative stress in HaCaT cells. In conclusion, oxidative damage is positively correlated with inflammatory factors and disease severity of AD and HO-1 may be potential target as its antioxidant and anti-inflammation effects.

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“…To date, there is no report on the production of these growth factors in the infection of keratinocytes by Sporothix spp. However, the production of GM-CSF and G-CSF has been reported in keratinocytes treated with dinitrochlorobenzene, and they have been attributed a pro-inflammatory effect [ 65 ], which could be contributing to the pathogenesis of Sporotrichosis.…”

Section: Discussionmentioning

confidence: 99%

Keratinocyte Response to Infection with Sporothrix schenckii

Paredes-Rojas

Palma-Ramos

Castrillón-Rivera

et al. 2022

JoF

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Sporotrichosis is a subacute, or chronic mycosis caused by traumatic inoculation of material contaminated with the fungus Sporothrix schenckii which is part of the Sporothrix spp. complex. The infection is limited to the skin, although its progression to more severe systemic or disseminated forms remains possible. Skin is the tissue that comes into contact with Sporothrix first, and the role of various cell lines has been described with regard to infection control. However, there is little information on the response of keratinocytes. In this study, we used the human keratinocyte cell line (HaCaT) and evaluated different aspects of infection from modifications in the cytoskeleton to the expression of molecules of the innate response during infection with conidia and yeast cells of Sporothrix schenckii. We found that during infection with both phases of the fungus, alterations of the actin cytoskeleton, formation of membrane protuberances, and loss of stress fibers were induced. We also observed an overexpression of the surface receptors MR, TLR6, CR3 and TLR2. Cytokine analysis showed that both phases of the fungus induced the production of elevated levels of the chemokines MCP-1 and IL-8, and proinflammatory cytokines IFN-α, IFN-γ and IL-6. In contrast, TNF-α production was significant only with conidial infection. In late post-infection, cytokine production was observed with immunoregulatory activity, IL-10, and growth factors, G-CSF and GM-CSF. In conclusion, infection of keratinocytes with conidia and yeast cells of Sporothrix schenckii induces an inflammatory response and rearrangements of the cytoskeleton.

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Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte

Cited by 16 publications

References 47 publications

Oxidative Stress and Gut Microbiome in Inflammatory Skin Diseases

Oxidative Stress and Gut Microbiome in Inflammatory Skin Diseases

WITHDRAWN: HO-1 may be the potential therapeutic target for Atopic dermatitis as oxidative stress is dysregulated and associated with inflammation in Atopic dermatitis patients

Keratinocyte Response to Infection with Sporothrix schenckii

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