Spectrophotometric and spectrofluorimetric determination of some drugs containing secondary amino group in bulk drug and dosage forms via derivatization with 7-Chloro-4-Nitrobenzofurazon

Abstract

Sensitive and selective spectrophotometric and spectrofluorimetric methods have been developed for determination of some drugs such as Pramipexole, Nebivolol, Carvedilol, and Eletriptan, which commonly contain secondary amino group. The subject methods were developed via derivatization of the secondary amino groups with 7-Chloro-4-Nitrobenzofurazon in borate buffer where a yellow colored reaction product was obtained and measured spectrophotometrically or spectrofluorimetrically. Concentration ranges were found as 2.0 to 250 μg mL-1 and 0.1 to 3.0 μg mL-1, for spectrophotometric and spectrofluorimetric study, respectively. The described methods can be easily applied by the quality control laboratories in routine analyses of these drugs in pharmaceutical preparations

Spectrophotometric quantification of direct factor xa inhibitor, rivaroxaban, in raw and tablet dosage form

“…NBD has been used as a fluorogenic or chromogenic reagent in pharmaceutical analysis. The analysis of different compounds/drugs either in pharmaceuticals/biological samples was performed after derivatization with NBD followed by measuring the resulted product by means of spectrophotometry [33][34][35][36][37][38] and spectrofluorimetry [39,40]. Due to electrophilic properties, NBD has also been used in charge transfer reactions.…”

Spectrofluorimetric Determination of Atenolol and Carvedilol in Pharmaceutical Preparations after Optimization of Parameters using Response Surface Methodology

“…17 Fluorescence spectrometry due to its low cost as well as great sensitivity and selectivity is widely used in quantitative analysis of different materials such as drugs, [18][19][20][21][22] thus several spectrofluorimetric methods have been proposed for the determination of ATE and CAR in their pharmaceutical preparation. [22][23][24][25][26][27][28][29] In practice, usually trial and error or simple optimization such as one parameter at a time is used to find the best analytical conditions, but these are time-consuming procedures. Multivariate experimental design strategies are useful methods for the optimization of a response, i.e., the experimental conditions that produce the best results.…”

High Performance Liquid Chromatographic Methods for Analysis of Taurine in Energy Drinks after Pre-column Derivatization

“…4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) is an extremely sensitive labeling agent that have been used for the determination of primary and secondary amino acids and some drugs [12]. NBD-Cl react amino group to form stable condensation colored products [19]. NBD-Cl has advantages such as low cost, it can be used for fluorescent labeling of amino acids and UV detection, and this reagent produces low number of byproducts [20].…”