“…Other techniques use the efflux of the acid phosphatase (EC 3.1.3.2) as an effective biomarker. The activity of the acid phosphatase on different organisms has been used to determine pollutant effects on algae (Jonsson and Aoyama 2010;Pereira et al 2012), Daphnia magna (Khangarot and Rathore 2003) and several species of fishes (Vijayavel and Balasubramanian 2007;Nazar et al 2008). The acid phosphatase activity has also been taken as parameter for the evaluation of toxic effects of other chemicals and priority pollutants.…”