Estimation of Biodiesel Cytotoxicity by Using Acid Phosphatase as a Biomarker of Lysosomal Integrity

Cruz, Andrea; Leite, Maria Bernadete Neiva Lemos; Rodrigues, Luiz Erlon; Nascimento, Iracema Andrade

doi:10.1007/s00128-012-0707-7

Cited by 17 publications

(13 citation statements)

References 24 publications

(42 reference statements)

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“…Information regarding biofuel exhaust particles, their chemical composition, size, morphology, biological and toxicological effects is scarce (Traviss et al, 2014). Biodiesel could present deleterious effects on the aquatic environments (da Cruz et al, 2012) and could exert undesirable effects on the human respiratory tract (Bünger et al, 2012). A review of literature showed that there are few toxicological, cytotoxicity and mutagenicity studies on the effects of biodiesel exhaust in biological systems.…”

Section: Introductionmentioning

confidence: 99%

Biodiesel from soybean promotes cell proliferation in vitro

Gioda

Rodríguez-Cotto

Amaral

et al. 2016

Toxicology in Vitro

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Toxicological responses of exhaust emissions of biodiesel are different due to variation in methods of generation and the tested biological models. A chemical profile was generated using ICP-MS and GC-MS for the biodiesel samples obtained in Brazil. A cytotoxicity assay and cytokine secretion experiments were evaluated in human bronchial epithelial cells (BEAS-2B). Cells were exposed to polar (acetone) and nonpolar (hexane) extracts from particles obtained from fuel exhaust: fossil diesel (B5), pure soybean biodiesel (B100), soybean biodiesel with additive (B100A) and ethanol additive (EtOH). Biodiesel and its additives exhibited higher organic and inorganic constituents on particles when compared to B5. The biodiesel extracts did not exert any toxic effect at concentrations 10, 25, 50, 75, and 100 μg mL -1. In fact quite the opposite, a cell proliferation effect induced by the B100 and B100A extracts is reported. A small increase in concentrations of inflammatory mediators (Interleukin-6, IL-6; and Interleukin-8, IL-8) in the medium of biodiesel-treated cells was observed, however, no statistical difference was found. An interesting finding indicates that the presence of metals in the nonpolar (hexane) fraction of biodiesel fuel (B100) represses cytokine release in lung cells. This was revealed by the use of the metal chelator. Results suggest that metals associated with biodiesel’s organic constituents might play a significant role in molecular mechanisms associated to cellular proliferation and immune responses.

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Section: Introductionmentioning

confidence: 99%

Biodiesel from soybean promotes cell proliferation in vitro

Gioda

Rodríguez-Cotto

Amaral

et al. 2016

Toxicology in Vitro

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“…The reversibility of the transesterification process produces methanol, a water soluble compound that has been reported to elicit cytotoxic effects by altering acid phosphatase activity in multi-cellular organisms [45] and also able to reduce microbial growth at high concentrations in the soil [46]. Studies by Sannino and Gianfreda [47] also corroborate the stimulatory and inhibitory effects of methanol on soil phosphatase enzyme activities.…”

Section: Toxicity Assessment Of Stepwise Amendment Experiments By Phomentioning

confidence: 85%

Enhanced Degradation of Benzo[α]Pyrene in Coal Tar Contaminated Soils Using Biodiesel

To¹,

Dm²

2018

J Bioremediat Biodegrad

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The biodegradation of the potent carcinogen, benzo [a]pyrene (BaP), and other priority pollutants was investigated in un-weathered coal tar contaminated soil over a period of 150 days. Results from the laboratory microcosm experiments showed that after 60 days, the concentrations of BaP were significantly reduced by 81%, in the biodiesel amended samples compared to the 26% and 34% depletion in the control and nutrient-only amended microcosms, respectively. The 3-ring PAH anthracene was also almost completely biodegraded in the presence of biodiesel. However, phenanthrene degradation was significantly inhibited in these samples as only 3% reduction occurred as opposed to the 80% depletion observed in the control. A stepwise treatment approach conducted on the coal tar spiked soil also revealed a higher reduction in BaP (98%) in the biodiesel amended microcosms compared to the control (29%) and further enhanced the depletion of phenanthrene by 51% after 60 days of adding biodiesel to soil initially treated with nutrients. Toxicity assays showed that biodiesel amended microcosms stimulated phosphatase enzyme activity and exhibited a lower toxic response to Microtox Vibrio fischeri. Overall, the pattern observed in the removal of the PAHs using biodiesel, suggests the co-metabolic action of ligninolytic fungi, probably via lignin peroxidases, as also evidenced from the visible growth of moulds after 14 days of amendment. The enhanced removal of carcinogenic PAH and the reduced toxicity observed in soil after biodiesel amendment, indicates that this bioremediation technique has potential for full scale field trials.

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“…The chromatographic data were obtained using a gas chromatograph (Varian, model CP3800) with a flame ionization detector and a capillary column. In addition, electrical conductivity (Gehaka, (Cruz et al 2012). For the tests, they were randomly distributed in glass aquariums at a density of 1 fish/4 L of the test-solutions, which consisted of the water-soluble fractions (WSF) of the different fuels (B100, B5 and diesel), distributed in test containers according the previously established standard operation procedures (SOPs), inserted in the QA/QC Laboratory Program (Nascimento et al 2002;Cruz et al 2012).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, electrical conductivity (Gehaka, (Cruz et al 2012). For the tests, they were randomly distributed in glass aquariums at a density of 1 fish/4 L of the test-solutions, which consisted of the water-soluble fractions (WSF) of the different fuels (B100, B5 and diesel), distributed in test containers according the previously established standard operation procedures (SOPs), inserted in the QA/QC Laboratory Program (Nascimento et al 2002;Cruz et al 2012). The tests involved the fishes exposure to a serial dilution of each product-WSF at concentrations of 0 % (control), 4.6 %, 10 %, 22 %, 46 % and 100 % aiming at determining their effective concentrations (Leite et al 2011;Pereira et al 2012).…”

Section: Methodsmentioning

confidence: 99%

Comparing the Toxicity of Water-Soluble Fractions of Biodiesel, Diesel and 5 % Biodiesel/Diesel Blend on Oreochromis niloticus Using Histological Biomarkers

Leite

Cruz

Rodrigues

et al. 2015

Bull Environ Contam Toxicol

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This study estimated end compared the potential toxic effects of the water-soluble fractions (WSF) of biodiesel (B100), diesel and the commercial biodiesel (B5) on Oreochromis niloticus. After a 24 h-exposition to WSF-0% (control) and WSF-serial concentrations of 4.6%, 10%, 22%, 46% and 100%, samples of gill and liver of the exposed fishes were fixed in Bouin's solution, processed, stained using hematoxylin/eosin and analyzed by light-microscopy. WSF-hydrocarbons and methanol contents, analyzed by gas chromatography, were checked against the occurrence of abnormal histopathological alterations. These were not found in the control and WSF-4.6% exposed fishes, while exposures to or above 10%-WSF resulted in histopathological alterations whose severity increased in a dose-dependent manner, being higher in fishes exposed to WSF-diesel, or WSF-B5 when compared to biodiesel. These results, which were corroborated by the chemical analyses, highlighted the histological technique as an appropriate diagnostic tool that can be used for the preservation of water bodies' quality.

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Estimation of Biodiesel Cytotoxicity by Using Acid Phosphatase as a Biomarker of Lysosomal Integrity

Cited by 17 publications

References 24 publications

Biodiesel from soybean promotes cell proliferation in vitro

Biodiesel from soybean promotes cell proliferation in vitro

Enhanced Degradation of Benzo[α]Pyrene in Coal Tar Contaminated Soils Using Biodiesel

Comparing the Toxicity of Water-Soluble Fractions of Biodiesel, Diesel and 5 % Biodiesel/Diesel Blend on Oreochromis niloticus Using Histological Biomarkers

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