In vitro study of antioxidant and scavenger properties of phenolic compounds from Lychnophora species

Grael, Cristiane Fernanda Fuzer; Kanashiro, Alexandre; Kabeya, Luciana M.; Jordão, Christiane O.; Takeara, Renata; Gobbo‐Neto, Leonardo; Polizello, Ana Cristina Morseli; Lucisano-Valim, Yara Maria; Lopes, Norberto P.; Lopes, J L C

doi:10.1590/s0100-40422010000400019

Cited by 18 publications

(11 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Comparison with the fragmentation profiles of chlorogenic acids described in the literature supported our identification of this type of compound [25][26][27][28]. As for the other compounds present in the evaluated extract, accurate mass data and comparison with previously described standards isolated by our research group were essential for their complete structural elucidation [3,4,12,[29][30][31] (for complete details and access to the original spectra, see supplementary material).…”

Section: Resultssupporting

confidence: 70%

A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicão) leaves: Application to chemical variability evaluation

Gouvea¹,

Meloni²,

Ribeiro³

et al. 2012

Analytica Chimica Acta

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 70%

A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicão) leaves: Application to chemical variability evaluation

Gouvea¹,

Meloni²,

Ribeiro³

et al. 2012

Analytica Chimica Acta

View full text Add to dashboard Cite

“…Phenolic compounds have antioxidant properties in plant metabolism (Grael et al, 2010). Therefore, phenolic compound accumulation could be a means of reducing the oxidative stress induced by arsenic (Zhao et al, 2010;Nascimento et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Arsenic toxicity in Acacia mangium willd. and mimosa Caesalpiniaefolia benth. seedlings

Cipriani

Dias²,

Costa³

et al. 2013

Rev. Bras. Ciênc. Solo

View full text Add to dashboard Cite

SUMMARYAcacia mangium and Mimosa caesalpiniaefolia are fast-growing woody fabaceous species that might be suitable for phytoremediation of arsenic (As)-contaminated sites. To date, few studies on their tolerance to As toxicity have been published. Therefore, this study assessed As toxicity symptoms in A. mangium and M. caesalpiniaefolia seedlings under As stress in a greenhouse. Seedlings of Acacia mangium and M. caesalpiniaefolia were grown for 120 d in an Oxisol-sand mixture with 0, 50, 100, 200, and 400 mg kg -1 As, in four replications in four randomized blocks. The plants were assessed for visible toxicity symptoms, dry matter production, shoot/root ratio, root anatomy and As uptake. Analyses of variance and regression showed that the growth of A. mangium and M. caesalpiniaefolia was severely hindered by As, with a reduction in dry matter production of more than 80 % at the highest As rate. The root/shoot ratio increased with increasing As rates. At a rate of 400 mg kg -1 As, whitish chlorosis appeared on Mimosa caesalpiniaefolia seedlings. The root anatomy of both species was altered, resulting in cell collapse, death of root buds and accumulation of phenolic compounds. Arsenic concentration was several times greater in roots than in shoots, with more than 150 and 350 mg kg -1 in M. caesalpiniaefolia and A. mangium roots, respectively. These species could be suitable for phytostabilization of As-contaminated sites, but growth-stimulating measures should be used.Index terms: arsenate, heavy metals, phytoremediation, root anatomy, soil contamination.(

show abstract

“…The phenolic and flavonoid are secondary antioxidant compounds normally produced by plants to defend themselves under stress condition by chelating the metals that interfere normal plant processes (Michalak, 2006), as well as inhibit ROS generation (Deng et al, 1997;Grael et al, 2010). Although there was a significant increase in total phenolic content with increasing copper concentrations, the percentage of increment seems low, probably due to low level of oxidative stress in cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of Copper on Total Phenolics, Flavonoids and Mitochondrial Properties of Orthosiphon stamineus Callus Culture

Mamat

ChunShiong²,

Samad³

et al. 2015

IJAB

View full text Add to dashboard Cite

Plants need an appropriate amount of copper to sustain their normal growth. However, excess of copper may interrupt plant development and cause stress. In this study, the responses of Orthosiphon stamineus callus culture under various copper concentrations were investigated. Callus cells at 24-days old were treated with 100, 150, 200 and 250 µM of copper solution and harvested after one week. Mitochondria from the calluses were isolated and the total mitochondrial proteins were determined. We used sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to compare the mitochondrial protein profiles in normal medium and copper-containing medium. The activities of cytochrome c oxidase, as well as the total phenolic and flavonoid contents were determined. The results of atomic absorption spectroscopy (AAS) analysis have shown significant amount of copper accumulated in O. stamineus callus cells, depending on the amount of copper supplied. With the BCF values of more than 10, this species can be categorized as copper hyperaccumulator. When the amount of copper supplied increased, the cells exhibit higher cytochrome c oxidase activities and produce more phenolic compounds, in spite of decreasing amount of total mitochondrial proteins and a lesser number of visible bands visualized from SDS-PAGE analysis. Overall, our findings highlight the dynamic responses of O. stamineus cells when exposed to various levels of copper, notably by alteration of biochemical and mitochondrial properties as their protection mechanism under stress.

show abstract

In vitro study of antioxidant and scavenger properties of phenolic compounds from Lychnophora species

Cited by 18 publications

References 13 publications

A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicão) leaves: Application to chemical variability evaluation

A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicão) leaves: Application to chemical variability evaluation

Arsenic toxicity in Acacia mangium willd. and mimosa Caesalpiniaefolia benth. seedlings

Effects of Copper on Total Phenolics, Flavonoids and Mitochondrial Properties of Orthosiphon stamineus Callus Culture

Contact Info

Product

Resources

About