A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicão) leaves: Application to chemical variability evaluation

Gouvea, Dayana Rubio; Meloni, Fernando; Ribeiro, Arthur de Barros Bello; Lopes, João Luís Callegari; Lopes, Norberto Peporine

doi:10.1016/j.aca.2012.08.027

Cited by 19 publications

(12 citation statements)

References 27 publications

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“…Initially, a systematic investigation concerning the effects of energy and frequency laser, number of shots and PIE was performed to develop the metabolic fingerprint method by LDI-MS, since this approach had not been explored previously. So a qualitative screening of L. salicifolia specimens was performed and it was consistent with previous investigations7918, confirming the ability of the LDI-MS proposed method to analyze L. salicifolia specimens. Moreover, the flavonoid vicenin-2 was found in all specimens regardless of the origin of the plants.…”

Section: Discussionsupporting

confidence: 87%

“…So, it explains the distribution of vicenin-2 producing a layer on the top of the leaf, which set off from epidermal cells in upper up to lower leaf sides; different distribution in the tissue can be related to the position of the leaves in the plants. Based on the distribution and its absorption coefficient7, vicenin-2 probably acts as a sunlight barrier, mainly against UV-B radiation. The radiation is divided into lower energy UV-A (320–400 nm), higher energy UV-B (280–320 nm) and UV-C (254–280 nm), but UV-B causes the most severe damages2324.…”

Section: Discussionmentioning

confidence: 99%

“…The survival characteristic of these plants under these evolutionary pressures can be related to these strong environmental characteristics. Recently, an ecological analysis of Lychnophora salicifolia showed a correlation between the geographical localization of a sample and its polar metabolites (quantitative effects), such as vicenin-2, but no qualitative differences7. Vicenin-2 (Fig.…”

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Mass Spectrometry of Flavonoid Vicenin-2, Based Sunlight Barriers in Lychnophora species

Silva

Turatti

Gouveia

et al. 2014

Sci Rep

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Lychnophora salicifolia plants collected from four different places in Brazil (three states: Goias, Minas Gerais and Bahia) revealed a conserved accumulation of vicenin-2, a di-C-glycosyl flavonoid. Quantitative studies by UPLC-MS/MS showed high concentration of vicenin-2 in leaves from sixty specimens of six Lychnophora species. So the tissue distributions of vicenin-2 were evaluated in wild Lychnophora leaves (Asteraceae) by laser based imaging mass spectrometry (IMS) to propose its distributions and possible functions for the species analyzed. Mass spectrometric imaging revealed that vicenin-2, unlike other flavonoids, was produced at the top of the leaves. The combination of localization and UV absorption properties of vicenin-2 suggests that it could act as a UV light barrier to protect the plants, since plants are sessile organisms that have to protect themselves from harsh external conditions such as intense sunlight.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Mass Spectrometry of Flavonoid Vicenin-2, Based Sunlight Barriers in Lychnophora species

Silva

Turatti

Gouveia

et al. 2014

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“…[2][3][4][5] These compounds are present in large amounts in hydroalcoholic extract from leaves of Lychnophora salicifolia and are present in most studied populations of this plant. 6 VIC and LA structures are illustrated in Figure 1.…”

Section: Introductionmentioning

confidence: 99%

An UPLC-MS/MS Method for Determination of Vicenin-2 and Lychnopholic Acid in Rat Plasma and its Application to a Pharmacokinetic Study

Gouvea¹,

Buqui²,

Lopes³

et al. 2016

J. Braz. Chem. Soc.

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Vicenin-2 (VIC) and lychnopholic acid (LA) are present in large amounts in hydroalcoholic extract from leaves of Lychnophora salicifolia, commonly known in the Brazilian Cerrado as "arnicão", a plant applied as topic anti-inflammatory and as a flavoring agent for the traditional spirit (cachaça). A new ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for quantification of LA and VIC in plasma samples in order to evaluate their pharmacokinetic profiles in rats. The method was linear in the range of 10-2500 ng mL -1 , acceptable precision accuracies were obtained and no significant matrix effect was observed. The validated method was successfully applied to pharmacokinetic study. LA presented very low t 1/2 (half-time), high Cl (serum clearance) and Vd (volume of distribution), and for VIC, the t 1/2 value was slightly larger, but still rather low, whereas Cl and Vd values were slightly lower, indicating that both substances are instantaneous and well distributed in the body, and quickly eliminated.

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“…The identity of the peak is thought to be the sodiated ion of vicenin-2, which already was identified for Lychnophora salicifolia (GOUVEA et al, 2012). In spectra acquired without the addition of the NaCl solution a peak with only a very low intensity, barely distinguishable from noise could be observed at m/z 595, where the protonated ion of vicenin-2 would appear (see Figures 32 and 33).…”

Section: Resultsmentioning

confidence: 99%

Metabolômica como ferramenta em taxonomia: O modelo em Arnica. Metabolomics in plant taxonomy: The Arnica model

Ernst¹

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A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicão) leaves: Application to chemical variability evaluation

Cited by 19 publications

References 27 publications

Mass Spectrometry of Flavonoid Vicenin-2, Based Sunlight Barriers in Lychnophora species

Mass Spectrometry of Flavonoid Vicenin-2, Based Sunlight Barriers in Lychnophora species

An UPLC-MS/MS Method for Determination of Vicenin-2 and Lychnopholic Acid in Rat Plasma and its Application to a Pharmacokinetic Study

Metabolômica como ferramenta em taxonomia: O modelo em Arnica. Metabolomics in plant taxonomy: The Arnica model

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