Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE)

Abstract

Recebido em 3/2/09; aceito em 21/6/09; publicado na web em 25/11/09 OPTIMIZATION AND INTRALABORATORIAL VALIDATION OF METHOD FOR ANALYSIS OF CHLORAMPHENICOL RESIDUES IN GOAT MILK BY GC/ECD. The presence of chloramphenicol residues in goat milk can cause toxic effects in the population. The present work consists of the optimization and validation of analytical methodology for determination of chloramphenicol residues in goat milk by GC/ECD. The extraction was made with ethyl acetate and the clean-up with SPE-C18. The identification was made by comparison of retention time and GC/MS, and the quantification by external standard. The method was selective, linearity (0.998), precise (5.8-13.4%), exact (69.87-73.71%) and robust. The LOD and LOQ of method were 0.030 and 0.10 μg/kg, respectively. The method was efficiently for analysis of chloramphenicol in goat milk.

Study of the Reaction Derivatization Glyphosate and Aminomethylphosphonic Acid (AMPA) with <i>N</i>,O-Bis(trimethylsilyl)trifluoroacetamide

“…To favor the derivatization reaction with BSTFA one base was added to the medium. In this study, was used acetonitrile and pyridine, the latter is most often selected for derivatization reactions for analysis by GC [15,16].…”

Desenvolvimento e validação de método analítico para a determinação de resíduos de cloranfenicol e pescado por CLAE-ESI/EM/EM

“…Como forma de se determinar as condições de extração do analito cloranfenicol, -SPE) e dispensa da adição de dispersores nas etapas de trituração e secagem de amostras (MOTTIER et al, 2003;HUMMERT et al, 1995;DA SILVA et al, 2010).…”