In vitro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides Lam. Leaves

Abstract

In vItro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides lam. leaves Janaina dorneles mahlke, aline augusti boligon, michel mansur machado, tatiana borba spader, sydney Hartz alves, thais do canto-dorow e margareth linde athayde* Curso de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Campus Camobi, 97105-900 Santa Maria -RS, BrasilRecebido em 30/10/07; aceito em 16/9/08; publicado na web em 26/1/09Crude extract and fractions of Buddleja thyrsoides were investigated regarding antioxidant activities by DPPH, total phenolic contents by Folin-Ciocalteau and antimicrobial activity by the broth microdilution method. Total phenolics varied from 214.07 ± 3.6 to 438.4 ± 0.3 mg g -1 . Crude extract, ethyl acetate, dichloromethane and butanolic fractions exhibited a weak scavenging activity (SC 50 =186.04 ± 10.8, 137.70 ± 8.5, 146.89 ± 9.0 and 165.71 ± 3.2 μg mL -1 , respectively). A correlation between the antioxidant activities and total phenolic contents could be shown (r=0.857, p<0.01). The lowest value of MIC was observed with butanolic fraction against Saccharomyces cerevisiae (MIC and MFC at 62.5 μg mL -1 ). Dichloromethane and ethyl acetate fractions were effective against Staphylococcus aureus with MIC value at 250 and 500 μg mL -1 respectively.

Potential Use of Buddleja Thyrsoides for the Control and Prevention of American Foulbrood Disease in Honey Bees

“…In addition, previous works reported the antibacterial properties of B. thyrsoides against such diverse pathogens as Staphylococcus aureus, Saccharomyces cerevisiae, Pseudomonas aeruginosa, Candida glabrata, and Pro totheca zopfii (Mahlke et al, 2009). Based on earlier works and the present experiments with different fractions, an obvious antibacterial activity could be related to the chemical composition of the plant species.…”

“…Products derived from plants, such as propolis (Bastos et al, 2008;Mihai et al, 2012), essential oils (Albo et al, 2003;Santos et al, 2012); and extracts of Myrtus communis L., Eucalyptus dunnii (Maiden), Rosmarinus officinalis L., Zingiber officinale (Roscoe), Scutia buxifolia (Reissek), among other species (Flesar et al, 2010;Boligon et al, 2013a) were previously investigated and exhibited a growth-inhibitory effect against AFB. Buddleja is a genus with pan tropical distribution that occurs in South Asia, Africa, and America (Mahlke et al, 2009). Species belonging to the Buddleja genus exhibit antibacterial, antihepatotoxic, pesticidal, anti-inflammatory, sedative, analgesic, and diuretic actions (Houghton et al, 2003;Mahlke et al, 2012).…”

“…(Scrophulariaceae) is commonly known as "Barbasco" or "Cambara-do-campo", in Brazilian folk medicine, its leaves and flowers are taken by drinking an infusion made with hot water for the treatment of bronchitis and a cough (Mahlke et al, 2013). Some previously published work by Mahlke et al (2009;2013) describes the essential oil composition, antimicrobial, and antioxidant activities, as well as the antiplatelet and acetylcholinesterase inhibition, of B. thyrsoides. Our work is the first time that antimicrobial activity of B. thyrsoides crude extract and fractions against the Paenibacillus species have been evaluated.…”

In Vitro Evaluation of the Antimicrobial and Antimycobacterial Activities of Solanum guaraniticum A. St.-Hil. Leaves

“…These results can be explained, at least in part, because beside the efflux pumps, Gram-negative bacteria presents some other characteristic particularities in their outer membrane like the polysaccharides that contributes to cell surface properties, such as membrane permeability and antibiotic susceptibility (Mahlke et al, 2009). All the extracts were inactive against fungi, both as yeasts Candida albicans and Cryptococcus neoformans and for the filamentous fungi Aspergillus fumigatus.…”

PROFILING OF ANTI-FUNGAL ACTIVITY OF Trichoderma virens 159C INVOLVED IN BIOCONTROL ASSAY OF Ganoderma boninense

“…Usage of solvents with different polarities allowed discrimination between non-polar and polar fraction activities (Mahlke et al, 2009). The EtOAc extract of T. virens 159c effectively inhibited the activity of G. boninense PER 71 which might be due to the enrichment of anti-fungal compounds in the EtOAc extract without any interference of the other nonactive compound from the other fractions (Krishna Reddy et al, 2009).…”