Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P

Mertins, Omar; Sebben, Marcelo; Schneider, Paulo H.; Pohlmann, Adriana Raffin; Silveira, Nádya Pesce da

doi:10.1590/s0100-40422008000700043

Cited by 36 publications

(16 citation statements)

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“…For comparative purposes, purified lecithin was produced from crude soybean lecithin as previously described by Mertins et al 32 Briefly, 10 g of the granules of crude soybean lecithin was dispersed in ethyl acetate (100 mL) and ultrapure water (4 mL) was added under gentle shaking, leading to formation of a biphasic system. After decantation, the supernatant was discarded, and the lower phase was dispersed in acetone (60 mL).…”

Section: Purification Of Crude Lecithinmentioning

confidence: 99%

Co-encapsulation of Rifampicin and Isoniazid in Crude Soybean Lecithin Liposomes

et al. 2019

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Despite the well-known anti-mycobacterial actions of isoniazid (INH) and rifampicin (RIF), the clinical success of tuberculosis (TB) therapy requires prolonged administration of multiple drugs in high doses, which often result in frequent adverse effects and low patient adherence. Although liposomes are promising candidates for controlled delivery of anti-TB drug, the high cost of synthetic and highly purified natural lipids currently used in liposomal technology might preclude the universal application of therapeutic liposomes. This work aimed at evaluating the potential of a cost-effective lipid material, crude soybean lecithin (CL), to co-encapsulate RIF and INH for liposomal dual delivery. RIF was encapsulated in CL-liposomes with/without cholesterol using film hydration method, after which INH was incorporated using a freeze-thawing technique. Dynamic light scattering, differential scanning calorimetry, X-ray diffraction and dialysis were used for liposome characterization. Liposomes containing CL alone (CLL) exhibited 90 % encapsulation efficiency for RIF and 59 % for INH. The mean size and surface charge of CLL were 1114 nm and -63 mV, respectively. In addition, CLL showed a controlled release profile for the co-encapsulated drugs. CLL would be promising vehicles for macrophage-targeting drug delivery. The present findings demonstrate the feasibility of using CL for preparation of combination products for liposomal delivery.

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Section: Purification Of Crude Lecithinmentioning

confidence: 99%

Co-encapsulation of Rifampicin and Isoniazid in Crude Soybean Lecithin Liposomes

et al. 2019

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“…The phosphatidylcholine content of the ASO mixture is described by approximately 75% distearoylphosphatidylcholine (DSPC:C 18:0), 12% dioleoylphosphatidylcholine (DOPC:C 18:1) and 8% dipalmitoylphosphatidylcholine (DPPC:C 16:0). The vesicle size reduction could be related to the mixture of lipids found in ASO, which can be responsible for several phase states, from cylinder to hexagonal ones (Mertins et al, 2008;Malheiros et al, 2010).…”

Section: Mean Diameter Morphology Of Liposomes and Encapsulation Effmentioning

confidence: 99%

“…Liposomes, as model membranes, are also versatile tools to investigate a variety of physico-chemical properties or biological phenomena (De Lima, Morfim, Teixeira & Creczynski-Pasa, 2004). The amphiphilic nature of liposome components promotes the self-organization of bilayers, such as cell membranes (Mertins, Sebben, Schneider, Pohlmann & Silveira, 2008). Regarding the correlation of physico-chemical properties of liposomes and their biological activities, it would also be interesting to perform studies of unsaturated lipids, which can be used for preparing systems that resemble natural membranes, such as soybean asolectin (ASO), a mixture of phospholipids.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the encapsulation effect of phenolic compounds from Spirulina sp. LEB-18 on their antifusarium activities

et al. 2016

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“…Em tecidos animais, algumas das suas funções da membrana parecem ser compartilhadas com o esfingolipidio e com a esfingomielina(CRISTHIE, 2010). ParaMertins (2008), a fosfatidilcolina (FC) consiste de uma mistura de fosfolipídeos naturais constituídos por uma extremidade polar formada por um grupo colina e um grupo fosfato ligado à porção hidrofóbica. Devido à estrutura polarapolar, essas moléculas tendem a se auto-organizar em bicamadas As características de membranas utilizando FC vêm sendo estudadas no intuito de desenvolver novos sistemas para as mais diversas áreas, em especial nas ciências Esfingomielina (ou fosforilcolina ceramida) consiste de uma unidade de ceramida com uma fração de fosforilcolina.…”

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Análise do perfil lipídico do sêmen bovino

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DedicatóriaDedico, A meus pais, Vicente e Cirlene, pelo carinho, amizade e amor. Por serem responsáveis pelo que eu sou e por sempre acreditarem no meu sucesso.A minha irmã Ana Carolina pela sua amizade e incentivo, mesmo que por muitas vezes à distância. À Deus, por estar comigo em todos os momentos da minha vida. AgradecimentosÀ Universidade de São Paulo e ao Departamento de Anatomia dos Animais Domésticos e Silvestres, pela oportunidade de realização deste trabalho de pesquisa. Ao Prof. Felipe Perecin, pela oportunidade, pelo apoio e orientação. À CNPQ pela bolsa cedida, possibilitando a realização deste Mestrado. Aos meus pais, Vicente e Cirlene, pelo apoio e incentivo, à minha Irma Ana Carolina que torce por mim. À Dr. Christina Ramires Ferreira pela ajuda nas análises e obtenção dos espectros, pela disponibilidade e boa vontade em nos ajudar. Ao Laboratório Thomson do Instituto de Química da UNICAMP, pela realização das análises e pela obtenção das amostras. Ao Prof. Dr. Rubens Paes Arruda, pela colaboração com as amostras de sêmen para análise. A todos que, direta ou indiretamente, contribuíram para a realização deste trabalho. Palavras-chave: Lipídeos. Bovino. Espermatozoide. Espectrometria de massas.ABSTRACT FONSECA, M. A. Analysis of the lipid profile of bovine semen. [Análise do perfil lipídico do sêmen bovino]. 2012. 63 f. Dissertação (Mestrado em Ciências) -Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, 2012. The membrane lipid composition is amongst the factors that influence the fertilization competence of the spermatozoa. It is also known that lipids play fundamental roles at spermatozoa membranes, participating in many processes, such as spermatozoaoocyte interaction and determining the physical-chemical behavior of spermatozoa membranes during cryopreservation. These molecules show a wide range of chemical properties, making complex its analysis. Currently, mass spectrometry (MS) through matrix-assisted laser desorption ionization (MALDI) represents a powerful tool for lipids analysis. Hence, this proposal aimed to evaluate differences in bovine spermatozoa lipid profile obtained through MALDI using direct sample analysis of spermatozoa or analysis of spermatozoa lipid extract obtained by Bligh-Dyer technique. Moreover, this proposal evaluated lipid profile of spermatozoa from bull that received or not antioxidant supplementation. After collection, washing and separation, the spermatozoa were submitted to lipid extraction or straightforward destined to lipid profile acquisition (direct method). The lipid profiles of spermatozoa lipid extracts or from whole spermatoloza were obtained by MALDI in positive mode with DHB matrix (dihydrozybenzoic acid). Data analysis was performed with Metaboanalyst software using multivariated analysis (Principal component analysis -PCA) and tools for identification of differential ions between comparison groups (t-test, volcano plot). Results show differences in spermatozoa lipid profile obtained by direct method or after lipid...

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Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P

Cited by 36 publications

References 20 publications

Co-encapsulation of Rifampicin and Isoniazid in Crude Soybean Lecithin Liposomes

Co-encapsulation of Rifampicin and Isoniazid in Crude Soybean Lecithin Liposomes

Assessment of the encapsulation effect of phenolic compounds from Spirulina sp. LEB-18 on their antifusarium activities

Análise do perfil lipídico do sêmen bovino

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