High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma

Abstract

Recebido em 8/12/06; aceito em 23/4/07; publicado na web em 5/10/07 A rapid HPLC analytical method was developed and validated for the determination of the N-phenylpiperazine derivative LASSBio-579 in plasma rat. Analyses were performed using a C18 column and elution with 20 mM sodium dihydrogen phosphate monohydrate -methanol. The analyte was monitored using a photodiode array detector (257 nm). Calibration curves in spiked plasma were linear over the concentration range of 0.3-8 μg/mL with determination coefficient > 0.99. The lower limit of quantification was 0.3 μg/mL. The applicability of the HPLC method for pharmacokinetic studies was tested using plasma samples obtained after administration of LASSBio-579 to Wistar rats, showing the specificity of the method.