Enhanced triterpene production in Tabernaemontana catharinensis cell suspension cultures in response to biotic elicitors

Pereira, Paulo Sérgio; Ticli, Fábio Kiss; França, Suzelei de Castro; Breves, Camila Moniz de Souza; Lourenço, Miriam Vergínia

doi:10.1590/s0100-40422007000800010

Cited by 14 publications

(3 citation statements)

References 9 publications

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“…Addition of cell wall homogenates to the cell cultures, no matter the concentration or type of microorganism source tested, resulted in increased triterpene levels. Among elicitors tested, Saccharomyces cerevisae showed the best results in terms of triterpene accumulation [40].…”

Section: Changes In Saponin Production After Elicitationmentioning

confidence: 99%

Modulation of Triterpene Saponin Production: In Vitro Cultures, Elicitation, and Metabolic Engineering

Lambert

Faizal

Geelen

2011

Appl Biochem Biotechnol

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Saponins are secondary metabolites that are widely distributed in the plant kingdom and are often the active components in medicinal herbs. Hence, saponins have a potential for the pharmaceutical industry as antibacterial, virucidal, anti-inflammatory, and anti-leishmanial drugs. However, their commercial application is often hindered because of practical problems, such as low and variable yields and limited availability of natural resources. In vitro cultures provide an alternative to avoid problems associated with field production; they offer a system in which plants are clonally propagated and yield is not affected by environmental changes. Additionally, treatment of in vitro cultures with elicitors such as methyl jasmonate may increase the production of saponins up to six times. In vitro cultures are amenable to metabolic engineering by targeting specific genes to enhance saponin production or drive production towards one specific class of saponins. Hitherto, this approach is not yet fully explored because only a limited number of saponin biosynthesis genes are identified. In this paper, we review recent studies on in vitro cultures of saponin-producing plants. The effect of elicitation on saponin production and saponin biosynthesis genes is discussed. Finally, recent research efforts on metabolic engineering of saponins will also be presented.

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Section: Changes In Saponin Production After Elicitationmentioning

confidence: 99%

Modulation of Triterpene Saponin Production: In Vitro Cultures, Elicitation, and Metabolic Engineering

Lambert

Faizal

Geelen

2011

Appl Biochem Biotechnol

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“…The leaf extract is reported as antimicrobial, fungicidal, insecticidal, and nematocidal and the root extract is used to treat malaria, rheumatism, and skin rashes. [1][2][3][4][5] The highest concentration of OA in roots of Lantana camara. 6 Secondary metabolites are plant products that do not have any direct function in plants.…”

Section: Introductionmentioning

confidence: 99%

The Eff ect of Precursors and Elicitors on the Production of Oleanolic Acid in Suspension Culture of Lantana camara

Kamarapu¹,

Sailaja²

2023

IJPQA

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Oleanolic acid (OA) is an important pentacyclic triterpenoid. Traditionally used by Asian medicine, OA for anti-infl ammatory, anti-hyperlipidemic, and hepatoprotective. This study used triterpene precursors at 1 to 5 mg/L and elicitors in diff erent concentrations at 2, 5, 10 mg/L to enhance OA production in Lantana camara suspension cultures. Treated cells were evaluated for their OA contents by using HPLC and compared with the nontreated cells as a control. The results showed that FPP at 2 mg/L infl uenced the biomass and OA production more over the phytohormone combination, other precursors, and elicitors in Suspension cultures of L. camara.

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“…Several classes of compounds including xanthoids, iridoids, secoiridoids, trirepenoids, flavonoids, lignans, alkoloids and volatile constituents have been identified from the genus Swertia. Among these, pentacyclic triterpenes betulinic acid (BA), oleanolic acid (OA) and ursolic acid (UA) (Figure 1) are referred with enormous pharmacological potentials (Hsu et al 1997;Pereira et al 2007;Pai et al 2013). Traditional uses of plants containing these compounds in folk medicine are multiple in terms of anti-inflammatory, hepatic-protection, analgesic, cardiotonic, sedative and tonic effects .…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of three pentacyclic triterpenes from fiveSwertiaL. species endemic to Western Ghats, India, using RP-HPLC analysis

Kshirsagar

Pai

Nimbalkar

et al. 2015

Natural Product Research

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Aim of this study was to identify pentacyclic triterpenoids betulinic acid (BA), oleanolic acid (OA) and ursolic acid (UA) from five Swertia species endemic to Western Ghats, which are used as substitutes as well as adulterants to Swertia chirayita. Our results indicate that the concentration of active compound vary largely among and within the species from different localities. OA was found in a higher amount in all species compared to BA and UA. From the results, it was clear that BA, OA and UA are present in the endemic species collected from Western Ghats, thus advocating the use of these species as alternate sources to S. chirayita. This in due course may release pressure of exploitation from natural resources of S. chirayita and help to bring it out from an endangered category from conservation point of view.

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Enhanced triterpene production in Tabernaemontana catharinensis cell suspension cultures in response to biotic elicitors

Cited by 14 publications

References 9 publications

Modulation of Triterpene Saponin Production: In Vitro Cultures, Elicitation, and Metabolic Engineering

Modulation of Triterpene Saponin Production: In Vitro Cultures, Elicitation, and Metabolic Engineering

The Eff ect of Precursors and Elicitors on the Production of Oleanolic Acid in Suspension Culture of Lantana camara

Quantitative determination of three pentacyclic triterpenes from fiveSwertiaL. species endemic to Western Ghats, India, using RP-HPLC analysis

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