Enraizamento ex vitro e aclimatização de plântulas micropropagadas de amoreira-preta 'Xavante'

Pelizza, Tânia Regina; Muniz, Janaína; Camargo, Paola; Kretzschmar, Aike Anneliese; Rufato, Léo

doi:10.1590/s0100-29452013000100039

Cited by 3 publications

(7 citation statements)

References 7 publications

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“…In regards to aerial part fresh weight, root fresh weight, aerial part dry weight and root dry weight, there was no significant difference in regards to the tested treatment. Pelizza et al (2013), in an experiment with ex vitro mulberry rooting observed that an increase in IBA dosages led to an increase in fresh weight.…”

Section: Resultsmentioning

confidence: 99%

“…We believe that these results are related to the genotype physiological condition, since, under the same conditions, the Xavante cultivar showed no significant differences for the same testes variable. According to Pelizza et al (2013), there was a quadratic answer for the number of roots mean, in which the maximum efficiency point was obtained with the use of 320 mg.L -1 of IAB, producing 6.1 roots per seedling, for the ex vitro rooting of the Xavante blackberry seedlings.…”

Section: Resultsmentioning

confidence: 99%

“…Plant micropropagation stages include in vitro setting, multiplication, rooting and acclimatization (Pelizza et al, 2013). However, ex vitro rooting is most advantageous since, besides reducing cost and seedling time, the need for specialized labor is also low (Feyissa et al, 2007); however, there are several factors that may interfere in a successful ex vitro rooting such as temperature, relative humidity, substrate and the growth regulator (Pelizza et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Substrates and indolbutyric acid in ex vitro rooting of blackberry and raspberry mini-cuttings

Sommer

Camargo

Silva

et al. 2016

ASB J.

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The objective of this work was to determine the most adequate substrate and indolbutyric acid (IBA) concentration for the ex vitro rooting of the “Xavante” and “Tupy” blackberry and the “Heritage” and “Fall Gold” raspberry mini-cuttings. Explants were transferred to a laminar flow chamber and placed in a MS nutrient medium, giving origin to mini-cuttings whose bases immersed in four different IAC concentrations (0 mg.L-1, 1000 mg.L-1, 2000 mg.L-1, 3000 mg.L-1). Next, they were placed in transparent and articulated Sampack® (10 x 13 x 20) plastic bags with two types of substrates (vermiculite and vermiculite + coconut fiber, in the proportion of 1:1 v/v). For the number of roots variable, vermiculite substrate associated with coconut fiber showed representative results for the Tupy cultivar. However, for the Heritage cultivar, satisfactory results were obtained for the same variable with vermiculite substrate. One point of maximum efficiency was observed with the 1800 mg.L-1 IBA concentration. Results showed that the ex vitro rooting of blackberry and raspberry mini-cuttings may be realized without immersion in an IBA solution. Therefore, the use of vermiculite associated with coconut fibber is the most recommended.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Substrates and indolbutyric acid in ex vitro rooting of blackberry and raspberry mini-cuttings

Sommer

Camargo

Silva

et al. 2016

ASB J.

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“…Callus formation was not observed in any ex vitro plant as observed with blackberry after five minutes of immersion and ex vitro rooting (Pelizza et al, 2013). The callus formation reached 58.3% in vitro with IBA diluted in ethanol (Tab.…”

Section: Rootingmentioning

confidence: 91%

“…The rooting of microcuttings can either be performed in vitro, as is already widely used (Deng and Donnelly, 1993) or ex vitro during acclimatization, directly on the substrate (Jin et al, 1992;Augusto et al, 2006) or in float hydroculture (Clapa et al, 2013). Ex vitro rooting offers the advantage of reducing difficulties related to survival and development of plants cultivated in vitro (Augusto et al, 2006;Pelizza et al, 2013) and also reduces costs.…”

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confidence: 99%

Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

Bueno

Biasi

Tofanelli

2018

Rev. Colomb. Cienc. Hortic.

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This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

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In vitro propagation and seedling acclimatization of Caesalpinia ferrea Mart., a valuable medicinal plant in the Amazon (Fabaceae)

et al. 2020

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The objective of this work was to establish a protocol for the rapid in vitro multiplication of Caesalpinia ferrea Mart., aimed at genetic selection and in vitro germplasm conservation. Seeds treated in different sodium hypochlorite solution concentrations were inoculated in Murashige and Skoog (MS) culture medium and maintained in a growth room. After 30 days, sodium hypochlorite at 0.25% concentration resulted in 96.67% survival and 90% germination of the seeds. In the explants multiplication phase three types of medium culture were evaluated supplemented with cytokinins and auxins in different concentrations and combinations. The addiction of 0.1 mg·L-1 Benzylaminopurine (BAP) and 0.1 mg·L-1 Indole butyric acid (IBA) induced 100% of explants with shoots and 32% with root formation, while a complete absence of callus was found. It was observed that 90% of explants derived from the buds developed shoots with 2.73 cm and greater multiplication rate (10.06). The seedlings obtained from in vitro multiplication were acclimated without the presence of roots in different types of substrate. After 60 days, soil substrate was most indicated for acclimatization, showing 63.33% of healthy and uniform plants. In vitro propagation of C. ferrea allows the establishment and multiplication of in vitro seedlings from nodal segments and axillary buds.

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Enraizamento ex vitro e aclimatização de plântulas micropropagadas de amoreira-preta 'Xavante'

Cited by 3 publications

References 7 publications

Substrates and indolbutyric acid in ex vitro rooting of blackberry and raspberry mini-cuttings

Substrates and indolbutyric acid in ex vitro rooting of blackberry and raspberry mini-cuttings

Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

In vitro propagation and seedling acclimatization of Caesalpinia ferrea Mart., a valuable medicinal plant in the Amazon (Fabaceae)

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