Viabilidade "in vitro" de grãos de pólen de bacurizeiro - Clusiaceae

Neto, Francisco De Assis Sinimbú; Martins, Antônio Baldo Geraldo; Barbosa, José Carlos

doi:10.1590/s0100-29452011005000062

Cited by 7 publications

(2 citation statements)

References 6 publications

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“…The success of these methods depends on factors like the plant species and cultivar, plant nutritional status, season, time of the day, collection method, photoperiod, temperature, incubation period, and presence of micro and macronutrients in the culture medium (KAKANI et al, 2002;KAKANI et al, 2005;SOARES et al, 2008;CHAGAS et al, 2010;SINIMBÚ NETO et al, 2011). Several authors have used such methods to study pollen viability in maize (BARNABÁS et al, 1988;ALMEIDA et al, 2002;AYLOR, 2004, FERREIRA et al, 2007, DAVIDE et al, 2009.…”

Section: Introductionmentioning

confidence: 99%

In vitro pollen viability of maize cultivars at different times of collection

et al. 2017

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In the present study, we aimed to assess the in vitro viability of pollen grains from maize cultivars collected at different times and days in the field. Four cultivars (Sol da Manhã, XB 6012, XB 8010, and BRS 2020) were evaluated from the second to fifth day of anthesis in three times. Pollen samples were evaluated for their in vitro viability through standard germination test in liquid and solid media and tetrazolium staining. The experimental design was completely randomized, in 4x4x3 split-split plots with four replicates. Data were subjected to analysis of variance followed by a means clustering test and linear regression analysis. The average percentage of viable pollen varied according to the day, collection time, and cultivar. In general, XB 8010 and BRS 2020 had the highest frequency of viable pollen. The highest percentages of viable pollen were observed on the second day of anthesis at 10:00h.

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Section: Introductionmentioning

confidence: 99%

In vitro pollen viability of maize cultivars at different times of collection

et al. 2017

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“…It is recommended to test pollen viability before use (Einhardt, Correa, & Raseira, 2006). The success of in vitro germination depends on several factors such as plant species, plant nutritional status, time of year and time of collection, photoperiod, air temperature, collection method, incubation period and presence of micro and macronutrients in the culture medium (Soares et al, 2008), as well as adjustments of the culture medium composition for each species (Chagas, Pio, Chagas, Pasqual, & Bettiol Neto, 2010;Sinimbú Neto, Martins, & Barbosa, 2011).…”

Section: Introductionmentioning

confidence: 99%

Flower stages, germination and viability of pollen grains of Annona squamosa L. in tropical conditions

Araújo

Chagas

et al. 2021

Acta Sci Technol

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The success in the application of artificial pollination techniques, aspects of floral biology should receive special attention, especially regarding the viability studies of pollen grains. In this sense, two experiments were carried out, aiming to determine the floral stages: floral opening (anthesis), female, male and senescence stages of A. squamosa L flowers under tropical climate conditions, and to evaluate the germination and viability of pollen grains submitted to different storage conditions. In the first experiment, observations and data collection began when flowers were still closed. Readings were taken every two hours for 46 hours until all the flowers reached the senescence stage. For the second, the experimental design was completely randomized, in a 2 x 7 factorial scheme, with 2 storage conditions (environment at 27 ± 2°C and refrigerated at 5 ± 2°C) and 7 pollen grain conditioning times (0, 4, 8, 12, 16, 20 and 24 hours), with 4 repetitions for the analysis of pollen grain germination and 3 repetitions for the analysis of pollen grain viability, and each repetition consisted of one blade. The variables evaluated were percentage of viable and non-viable pollen grains and percentage of germinated and non-germinated pollen grains. For tropical climatic conditions, the flower anthesis (female stage) of the sugar-apple begins at 00:00 h, extending until 12:00h on the same day. The flowers of the tree reach a functionally staminate stage (male stage) from 6 a.m. the next day. Pollen grains stored at room conditions (27 ± 2°C) remain viable for up to 24 hours after collection, showing that storage in a cold environment at low temperatures (5 ± 2°C) is not necessary. Seed pollen grains have a germination percentage of 51.25% when stored under ambient conditions (27 ± 2°C) for up to 4 hours after collection.

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FT-IR Characterization of Pollen Biochemistry, Viability, and Germination Capacity inSaintpauliaH. Wendl. Genotypes

Buta

Cantor

Ştefan

et al. 2015

Journal of Spectroscopy

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FT-IR characterization of pollen biochemistry was analyzed to detect possible connection with the viability (by staining with potassium iodide, 25%) and the germination capacity (on solid nutrient medium), in 15Saintpauliagenotypes. Vibrational spectroscopy indicates that the pollen ofS. ionanthagenotype “Red Velvet” is rich in proteins, lipids, triglycerides, and esters and has a viability of 88.4% and a low germination capacity (27.16%). ForS. ionantha“Jolly Red” and “Lucky Ladybug” genotypes, pollen showed high viability (88.81–91.49%) and low germination capacity (23.02–9.17%), even though the pollen is rich in carbohydrates.S. ionantha“Aloha Orchid” genotype has the highest percentage of viability (94.32%) and germination capacity (45.73%) and a rich content of carbohydrates and polygalacturonic acids. InS. rupicolaandS. ionanthagenotypes, the rich content of polygalacturonic acids, lipids, and carbohydrates favourably influenced the germination capacity. Spectroscopic result indicates, through different absorbance band intensity, a possible link between biochemical composition, viability, and germination capacity ofSaintpauliapollen. To determine exactly the relation between biochemistry and biological processes, it is necessary to initiate quantitative researches.

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Viabilidade "in vitro" de grãos de pólen de bacurizeiro - Clusiaceae

Cited by 7 publications

References 6 publications

In vitro pollen viability of maize cultivars at different times of collection

In vitro pollen viability of maize cultivars at different times of collection

Flower stages, germination and viability of pollen grains of Annona squamosa L. in tropical conditions

FT-IR Characterization of Pollen Biochemistry, Viability, and Germination Capacity inSaintpauliaH. Wendl. Genotypes

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