FT-IR Characterization of Pollen Biochemistry, Viability, and Germination Capacity inSaintpauliaH. Wendl. Genotypes

Abstract

FT-IR characterization of pollen biochemistry was analyzed to detect possible connection with the viability (by staining with potassium iodide, 25%) and the germination capacity (on solid nutrient medium), in 15Saintpauliagenotypes. Vibrational spectroscopy indicates that the pollen ofS. ionanthagenotype “Red Velvet” is rich in proteins, lipids, triglycerides, and esters and has a viability of 88.4% and a low germination capacity (27.16%). ForS. ionantha“Jolly Red” and “Lucky Ladybug” genotypes, pollen showed high viability (88.81–91.49%) and low germination capacity (23.02–9.17%), even though the pollen is rich in carbohydrates.S. ionantha“Aloha Orchid” genotype has the highest percentage of viability (94.32%) and germination capacity (45.73%) and a rich content of carbohydrates and polygalacturonic acids. InS. rupicolaandS. ionanthagenotypes, the rich content of polygalacturonic acids, lipids, and carbohydrates favourably influenced the germination capacity. Spectroscopic result indicates, through different absorbance band intensity, a possible link between biochemical composition, viability, and germination capacity ofSaintpauliapollen. To determine exactly the relation between biochemistry and biological processes, it is necessary to initiate quantitative researches.

Analysis of morphological and molecular composition changes in allergenic Artemisia vulgaris L. pollen under traffic pollution using SEM and FTIR spectroscopy

“…Negative environmental stress strongly influences pollen grains, what is however not always mirrored in their morphology. It affects the pollen viability, germination, and finally reproduction success of the plant (Malayeri et al 2012; Buta et al 2015; Lahlali et al 2014). Kaur et al (2015) stated that the size and morphology does not depend on traffic, which yet, affects pollen viability.…”

“…Intensive studies on pollen biochemical compounds allowed creating a spectra library for many species, which enables the identification of pollen (Gottardini et al 2007; Dell’Anna et al 2009; Guedes et al 2014; Zimmermann 2010; Zimmermann et al 2015, 2016). Buta et al (2015) used FTIR to determine the links between the chemical structure, viability and germination of pollen of Saintpaulia genotypes. Zimermann and Kohler (2014) showed distinct inter-annual variations of biochemical constituents of Pinaceae specimens, which were explained by different course of weather in the period of pollen differentiation.…”

Discrimination of grass pollen of different species by FTIR spectroscopy of individual pollen grains

“…As demonstrated during the last decade, FTIR spectroscopy enables a detailed analysis of the species-specific chemical composition of pollen [14][15][16][17][18][19][20][21]. FTIR analysis of pollen is based on the fingerprint-like characteristics of the IR spectrum, containing contributions from all different kinds of biomolecular constituents.…”

Métodos colorimétricos sob uso de reagentes sintéticos e alternativos para viabilidade polínica de Bixa orellana L.

“…Há diferentes metodologias que podem ser aplicadas na verificação da viabilidade polínica, como por exemplo, os métodos colorimétricos que fazem uso de corantes, substâncias químicas específicas que interagem com elementos celulares nos grãos de pólen maduro e permitem estimar a viabilidade de uma espécie de maneira eficiente e rápida (Techio et al, 2006;Buta et al, 2015), mas para cada espécie podem ocorrer diferentes reações de coloração entre a planta e os corantes químicos (Lauton et al, 2016).…”