Caracterização de genes diferencialmente expressos na interação entre maçã 'Fuji' e Botryosphaeria dothidea

Abstract

RESUMO -O objetivo deste estudo foi investigar a interação entre o fungoBotryosphaeria dothidea e maçãs cv. Fuji por meio da técnica de Differential Display RT-PCR. O cDNA de frutos infectados e não infectados pelo fungo foi amplificado com uma combinação de 15 oligonucleotídeos iniciadores. Foram isolados 400 fragmentos de cDNA diferencialmente expressos, dos quais 120 foram sequenciados e comparados com sequências disponíveis no GenBank, por meio do programa BLASTX. As sequências obtidas foram similares à metalotioninas, profilina alergênica, proteína de resistência e fosfatase. Termos para indexação: Malus domestica, podridão-branca, transcriptase reversa. CHARACTERIZATION OF DIFFERENTLY EXPRESSED GENES DURING THE INTERACTION BETWEEN 'FUJI' APPLES AND Botryosphaeria dothideaABSTRACT -The aim of this study was to investigate the interaction between the Botryosphaeria dothidea fungi and 'Fuji' apples using the Differential Display RT-PCR technique. The cDNA of infected and non infected fruits by the fungus was amplified using a combination of 15 primers. Four hundred fragments of differentially expressed cDNA were isolated, and 120 of them were sequenced and compared with available sequences at GenBank database, using BLASTX software. Obtained sequences were similar to methalothionines, an allergenic profiline, a resistance linked protein, and a phosphatase.

Postharvest quality of ‘Brookfield’ apple field-treated with naphthalene acetic acid alone or combined with other growth regulators

“…During long-term storage period, decay incidence is one of the main causes of apple fruit loss (Corrent et al 2009;Vilanova et al 2012;Vilanova et al 2014). However, in the present study, no significant difference among treatments was measured (Fig.…”