Estabelecimento e multiplicação in vitro de Pyrus calleryana D-6 em sistema de cultura 'dupla-fase'

Moraes, Liziane Kadine Antunes de; Felisbino, Claudia; Crestani, Leandro; Silva, Aparecido Lima da

doi:10.1590/s0100-29452004000300008

Cited by 3 publications

(4 citation statements)

References 6 publications

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“…BAP is responsible for cell division, overcoming apical dominance, and induction of the proliferation of axillary buds (MOK et al, 2000). The present results are similar to those observed by Moraes et al (2004) in Pyrus calleryana D-6 and Radmann et al (2011) in the rootstock 'Flordaguard' (Prunus persica L. Bastch). Both studies found that in the absence of BAP, the emission of new shoots does not take place, creating a dependency on the addition of exogenous cytokinin for the induction of shoots in these two Rosaceae species.…”

Section: +supporting

confidence: 88%

“…However, they are limited by factors of the physical and chemical microenvironment in the growing containers, such as concentrations of nutrients and growth regulators that can endanger the formation of shoots and/or microplants (BOMMINENI et al, 2001;REED, 2002;CASANOVA et al, 2008). Consequently, the quantity and balance of growth regulators, carbohydrates, and other nutrients are determining factors in the success of in vitro multiplication of plant material (BELL; REED, 2002;RUZIC et al, 2008;MORAES et al, 2004;DOBRÁNSZKI;TEIXEIRA DA SILVA, 2010), as demonstrated by the results in this study.…”

Section: Discussionmentioning

confidence: 57%

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In vitro multiplication of pear tree cultivar Cascatense

et al. 2016

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One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L -1 ), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L -1 ) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L ResumoUm dos principais entraves ao desenvolvimento e expansão da cultura da pereira no Brasil tem sido a falta de cultivares adaptadas às condições edafoclimáticas das regiões potencialmente produtoras. Visando sanar esta dificuldade, a Embrapa Clima Temperado desenvolveu e lançou a cv. Cascatense, com baixa exigência em frio (~400 h), mais adequada ao clima do Rio Grande do Sul e de Santa Catarina. No entanto, mudas com alta qualidade genética e sanitária são necessárias para garantir a boa formação de um pomar produtivo. Plantas com qualidade sanitária e uniformidade podem ser obtidas através da micropropagação in vitro. Assim, o presente trabalho teve como objetivo verificar a influência de diferentes meios de cultivo (MS, MS¾, WPM, QL e Himédia), concentrações de sacarose (0, 15, 30, 45 e 60 g L -1 ) e benzilaminopurina -BAP (0; 0,4; 0,8; 1,2 e 1,6 mg L -1 ) na multiplicação in vitro da cv. Cascatense, visando obter um protocolo eficiente para potencializar sua propagação e reprodutibilidade. Os estudos foram conduzidos em três experimentos sequenciais, nos quais foram avaliados o número de brotações por explante, o comprimento das brotações, o número de gemas axilares por brotação, massa fresca e massa seca das brotações. A cv. Cascatense pode ser propagada in vitro com a utilização do meio Himédia suplementado com 0,8 mg L -1 de BAP e 30 g L -1 de sacarose. Palavras-chave: Meio himédia. Sulfato ferroso. Sulfato férrico. Sacarose. Benzilaminopurina.

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Section: +supporting

confidence: 88%

Section: Discussionmentioning

confidence: 57%

In vitro multiplication of pear tree cultivar Cascatense

et al. 2016

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“…3A and 4B. The double-phase culture medium is widely used in in vitro culture systems as it is more efficient for many species such as pear rootstock (Moraes et al 2004), and apple rootstock (Machado et al 2004). Surakshitha et al (2019) reported that the major physiological disorder observed during in vitro culture of woody plant species is shoot-tip necrosis (STN).…”

Section: Effect Of Culture Medium On Shoot Proliferationmentioning

confidence: 99%

“…Another factor that can contribute to increasing the multiplication rate and growth of explants is the use of 'double-phase' medium, as described for species of the genus Pyrus spp. (Rodríguez et al 1991, Kadota et al 2001, Moraes et al 2004 and Ananas sp. (Scherwinski-Pereira et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock

et al. 2021

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Flordaguard' has shown great potential as a standard rootstock for peach. The aim of this study was to examine the influence of Murashige & Skoog (MS) medium and modified MS-½N medium (half Nitrogen content), explant type and angle orientation on the culture medium, as well as 6-benzylaminopurine (BAP) concentrations on in vitro shoot proliferation in 'Flordaguard' rootstock. Explants cultured in MS-½N medium showed higher averages for number of lateral shoots and elongated shoots. The maximum average length of lateral shoots was obtained using doublephase medium. The 3.0 mg L -1 BAP induced the highest number of lateral shoots per explant (3.38 ±0.57). Whereas the higher number of lateral shoots elongated (1.75 ±0.10 cm) occurred at concentrations until 2.0 mg L -1 BAP. The study corroborated the importance of a 50% reduction in both NH 4 NO 3 and KNO 3 content in the cultured medium to improvein vitro propagation of 'Flordaguard' peach rootstock.

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In vitro Propagation of Kainth (Pyrus pashia) Using Explants from Forced Cutting

2015

Horticulture

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This study was carried out in Tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M 1 ), MS (M 2 ) and WPM (M 3 )} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Maximum establishment (63.60%) was obtained on M 2 containing BAP (1.5 mgl -1 ) and IBA (0.25 mgl -1 ). Maximum proliferated cultures (95.30%) and shoots per explant were obtained using M 3 medium fortified with BAP (3.0 mgl -1 ). However, shoots of maximum length (42.97 mm) were obtained in M 3 medium containing BAP (0.0 mgl-1) i.e control. Rooting (%), roots per explant and root length was found to be influenced by type of medium and growth regulator fortification. Rooting (%) was maximum (13.34%) was observed in M 1 medium supplemented with IBA (0.1 mgl -1 ). However, NAA (1.0 mgl -1 ) induced rooting of 29.61 per cent using M 1 medium. Maximum roots per explant were obtained using M 1 medium supplemented with IBA (1.0 mgl -1 ). NAA (1.0 mgl -1 ) induced highest roots per explant i.e 3.40 using M 1 medium. However, M 3 supplemented with IBA (0.1 mgl -1 ) resulted in maximum root length of 31.15 mm. NAA (0.1 mgl -1 ) resulted in maximum root length of 22.97 mm using M 1 medium.

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Estabelecimento e multiplicação in vitro de Pyrus calleryana D-6 em sistema de cultura 'dupla-fase'

Cited by 3 publications

References 6 publications

In vitro multiplication of pear tree cultivar Cascatense

In vitro multiplication of pear tree cultivar Cascatense

Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock

In vitro Propagation of Kainth (Pyrus pashia) Using Explants from Forced Cutting

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