Manitol para a conservação ex situ de coqueiro por crescimento mínimo

Abstract

O objetivo deste trabalho foi determinar a concentração de manitol para a germinação e crescimento mínimo in vitro do coqueiro (Cocos nucifera). Os seguintes acessos foram utilizados: Anão-vermelho-do brasil-de-gramame (BRDG), Anão-amarelo-da-malásia (MYD), Gigante-do-brasil-praia-do-forte (BRA), Gigante-da-polinésia (PYT) e Gigante-de-rennel (RIT). Os embriões foram colocados em meio de cultura Y3 a diferentes concentrações de manitol (0, 0,1, 0,2, 0,3 e 0,4 mol L-1). Verificou-se inibição do crescimento da parte aérea na presença de 0,1, 0,2 e 0,3 mol L-1, para MYD e BRDG, e de 0,1 e 0,2 mol L-1, para PYT, BRA e RIT. O uso do manitol é uma estratégia promissora para a conservação por crescimento mínimo.

In vitro maintenance, under slow-growth conditions, of oil palm germplasm obtained by embryo rescue

“…Similar results were found for coconut (Cocos nucifera L.) plants maintained in vitro at 27±1°C, for which the survival rate was 75% after 18 months of cultivation (García et al, 2008). Lédo et al (2007) observed, for this same species, the maintenance of 100% plant survival after 365 days of cultivation, and considered in vitro cultivation to be a promising alternative for the conservation of germplasm in palms. The use of low temperatures in the in vitro maintenance of the germplasm of palm species was previously reported only for date palm (Phoenix dactylifera L.) (Bekheet, 2011).…”

“…accessions after 12 months of in vitro storage at 25ºC with different carbohydrates at concentrations of 1, 2, and 3% (1) . (Lédo et al, 2007), in which the addition of mannitol resulted in lower growth of the aerial part and roots, significantly affecting plant survival. In the present study, the use of sorbitol produced intermediate results, but close to those obtained when the oil palm plants were cultivated in sucrose, with high explant survival, predominance of light-green leaves, and low incidence of necrosis.…”

“…In general, in vitro germplasm banks are maintained under minimal growth conditions, obtained by slowing down the plant metabolism, which is usually achieved by reducing temperature and light intensity, or by modifying the formulations of the culture media, including dilution of mineral elements, reduction of sugar concentrations, changes in the use of growth regulators, or the addition of osmotically active compounds (Lédo et al, 2007;Engelmann, 2011; Pesq. agropec.…”

“…agropec. bras., Brasília, v.50, n.5, p.426-429, maio 2015 DOI: 10.1590/S0100-204X2015000500010 -peng et al, 2012;Lédo et al, 2014;Nogueira et al, 2014). Currently, in vitro technology for the conservation of oil palm germplasm is focused almost exclusively on the technique of cryopreservation of zygotic embryos (Villa et al, 2007), somatic embryos (Konan et al, 2010), and pollen (Tandon et al, 2007).…”