Reaction of sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' infection

Felipe, Rafaella Teles Arantes; Filho, Francisco de Assis Alves Mourão; Lopes, S. A.; Mendes, Beatriz Madalena Januzzi; Behling, Maurel; Pereira, Ernani Vicente

doi:10.1590/s0100-204x2013001100004

Cited by 9 publications

(7 citation statements)

References 21 publications

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“…Therefore, many researchers are committed to detecting plants in Rutaceae that harbor immunity, resistance, or tolerance to the bacterium or the psyllid vector (Folimonova et al 2009;Bowman et al 2016;Ramadugu et al 2016). Developing HLB-resistant plants using genetic engineering is another strategy (Felipe et al 2013;Dutt et al 2015;Alquézar et al 2017;Miyata et al 2017;Zou et al 2017;Guerra-Lupián et al 2018;Tavano et al 2019). These are long-term strategies and, to date, a variety that is resistant to HLB has not been developed.…”

Section: Attempts To Cure or Remedy Disease Symptomsmentioning

confidence: 99%

Overview of citrus huanglongbing spread and management strategies in Brazil

Bassanezi

Lopes

Miranda

et al. 2020

Trop. plant pathol.

104

133

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Huanglongbing (HLB) has been a serious threat to the citrus industry worldwide. After its first report in São Paulo State, the main citrus production area in Brazil, the disease spread to the States of Minas Gerais, Paraná and Mato Grosso do Sul. Attempts to cure plants or remiss disease symptoms and damages have been evaluated and showed to be inefficient and nonviable. The development of resistant or tolerant varieties to the bacteria or its insect vector, the Asian citrus psyllid Diaphorina citri, is still a long term challenge. Earlier HLB management has been based on preventive measures such as planting of healthy nursery trees, elimination of diseased trees, and vector control. Supported by both research data and citrus grower experiences, HLB management in São Paulo and Triângulo/Sudoeste Mineiro citrus belt has been improved from measures individually applied only into the orchards to regional disease management, including differentiated psyllid control in the orchards based on tree location and shoot flushing, area-wide coordinated control of psyllids, and removal of inoculum sources in noncommercial properties in the vicinity of commercial orchards. In addition, the negative impact of HLB on orchard production and longevity has been reduced with wide adoption of better cultural practices such as high-density planting, irrigation, and adequate nutrition. Unlike in other countries where HLB reached epidemic levels, the management of HLB in São Paulo and Triângulo/Sudoeste Mineiro citrus belt has been considered a success case and has ensured the maintenance of citrus production and competitiveness of the Brazilian citrus industry while new, more durable, and sustainable measures are not yet available.

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Section: Attempts To Cure or Remedy Disease Symptomsmentioning

confidence: 99%

Overview of citrus huanglongbing spread and management strategies in Brazil

Bassanezi

Lopes

Miranda

et al. 2020

Trop. plant pathol.

104

133

View full text Add to dashboard Cite

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“…Transgenic lines expressing attA transgene showed some level of tolerance to HLB with lowered CLas titer estimated by qPCR. One of the lines also had fewer visual HLB symptoms in comparison to the other lines and control (Felipe et al 2013). Contrary to what was observed by Boscariol et al (2006) and Cardoso et al (2010) with canker tolerance, attA lines were unable to provide durable resistance to HLB.…”

Section: Antimicrobial Peptide (Amp)-mediated Resistancementioning

confidence: 54%

“…Genetic transformation using attacin genes (att) have been successfully carried out in apple (attA, attE) and pear (attE) trees and resulted in a reduction in susceptibility to fire blight (Erwinia amylovora [Burr]) (Ko et al 2002;Reynoird et al 1999). To reduce the severity of citrus canker and HLB in citrus, several studies were conducted with genetically engineered plants expressing attacin A (attA) (Boscariol et al 2006;Cardoso et al 2010;Felipe et al 2013;Tavano et al 2019). 'Hamlin' sweet orange was stably transformed with an attA construct and citrus canker tolerance was evaluated in eight selected regenerated plants.…”

Section: Antimicrobial Peptide (Amp)-mediated Resistancementioning

confidence: 99%

Development of genetically modified citrus plants for the control of citrus canker and huanglongbing

Soares

Tanwir

Grosser

2020

Trop. plant pathol.

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Citrus cultivation is challenging due to the plethora of abiotic and biotic stresses faced by the crop. In recent years, production has been severely affected by diseases such as citrus canker and huanglongbing (HLB). Disease management is hampered as there is no field resistance to these diseases in any of the important commercially planted varieties. Traditionally, conventional breeding approaches have been applied for the improvement of the susceptible cultivars; however, this technique is laborious and time consuming. Genetic transformation of citrus allows for the rapid integration of novel genes into the plant's genome to develop disease-resistant transgenic plants. Therefore, efforts have been made to utilize genetic engineering tools to develop genetically modified citrus that are resistant to citrus canker and HLB. This review summarizes the major achievements made in the development of citrus canker and HLB tolerance using transgenic technologies.

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“…Although complete immunity may not be reached, transgenic sweet orange overexpressing genes encoding antimicrobial peptides or genes related to the SAR, may contribute to lower bacteria titers and, consequently, lower infection and epidemiological rates. In our previous work, five different transgenic lines of 'Pera' sweet orange expressing attacin A antimicrobial peptide under control of CaMV35S promoter showed significantly lower CLas titers than non-transgenic plants after bacterial infection through infected budwood (FELIPE et al, 2013).…”

Section: Resultsmentioning

confidence: 92%

PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus

et al. 2017

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-The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 (Citrus phloem protein 2), known to be expressed in Citrus sinensis phloem. 'Hamlin' sweet orange plants (Citrus sinensis L. Osbeck) transformed with the uidA (GUS) reporter gene under the control of AtPP2, AtSUC2 and CsPP2 promoters were infected to evaluate the interdependence between transgene expression and the concentration of Las. Plants were inoculated with Las by Diaphorina citri and eighteen months later, bacterial concentration and uidA expression were determined by qPCR and RT-qPCR, respectively. Reporter gene expression driven by AtSUC2 promoter was strongly and positively correlated with Las concentration. Therefore, this promoter combines desirable features of both tissue-specificity and pathogen-inducibility for the production of transgenic plants tolerant to Las. Index terms: Citrus sinensis, Diaphorina citri, genetic transformation, GUS, huanglongbing, quantitative real-time PCR. PROMOTORES DE FLOEMA SÃO DIFERENTEMENTE ATIVADOS POR Candidatus Liberibacter asiaticus EM LARANJEIRAS DOCES TRANSGÊNICASRESUMO -A utilização de promotores preferencialmente expressos em tecidos vegetais específicos é uma estratégia desejável na busca por resistência a patógenos que colonizam tais tecidos. A bacteria Candidatus Liberibacter asiaticus (Las), associada à doença huanglongbing (HLB) em citros, coloniza o tecido floemático. Alguns promotores, além de conferir expressão específica em certos tecidos, podem também responder diferencialmente à presença de patógenos. O objetivo deste estudo foi verificar se a presença de Las poderia modular a ativação dos promotores de floema AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) e CsPP2 (Citrus phloem protein 2), conhecidos por expressão específica em floema de Citrus sinensis. Plantas de laranja 'Hamlin' (Citrus sinensis L. Osbeck) transformadas com o gene reporter uidA (GUS) sob controle dos promotores AtPP2, AtSUC2 e CsPP2 foram infectadas para avaliar a interdependência entre a expressão dos transgenes e concentração de Las. Plantas foram inoculadas com Las por meio de Diaphorina citri e dezoito meses após, a concentração de bactéria e expressão do gene uidA foram determinadas por qPCR e RT-qPCR, respectivamente. A expressão do gene reporter dirigida pelo promotor AtSUC2 foi forte e positivamente correlacionada com a concentração de Las. Portanto, este promotor contém característ...

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Reaction of sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' infection

Cited by 9 publications

References 21 publications

Overview of citrus huanglongbing spread and management strategies in Brazil

Overview of citrus huanglongbing spread and management strategies in Brazil

Development of genetically modified citrus plants for the control of citrus canker and huanglongbing

PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus

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