Identificação morfológica e molecular de Helicoverpa armigera (Lepidoptera: Noctuidae) e ampliação de seu registro de ocorrência no Brasil

Abstract

Resumo -O objetivo deste trabalho foi descrever métodos para a caracterização morfológica e molecular de Helicoverpa armigera e ampliar o registro de ocorrência da praga no Brasil. As mariposas foram obtidas de lagartas coletadas nas culturas de algodão, milho e soja, com uso de armadilhas luminosas. As coletas foram realizadas na Bahia, no Distrito Federal, no Mato Grosso e no Paraná. A identificação foi baseada na genitália masculina e nas análises das sequências dos genes mitocondriais do citocromo B e da região cox1-tRNALeu-cox2. A genitália masculina foi comparada com as descrições morfológicas na literatura, e as sequências de genes, com as depositadas no GenBank. Ambas as análises confirmaram a presença de H. armigera nos locais de coleta. Ampliou-se o registro de ocorrência da praga para a região Sul do país.Termos para indexação: código de barras de DNA, lagarta polífaga, mtDNA, praga quarentenária. Morphological and molecular identification of Helicoverpa armigera (Lepidoptera: Noctuidae) and expansion of its occurrence record in BrazilAbstract -The objective of this work was to describe methods for molecular and morphological characterization of Helicoverpa armigera and to extend the pest occurrence record in Brazil. Moths were obtained from larvae collected in cotton, corn, and soybean crops, using light traps. Collections were done in the states of Bahia, Distrito Federal, Mato Grosso, and Paraná, Brazil. Identification was based on male genitalia and on the analyses of mitochondrial gene sequences of cytochrome B and of the cox1-tRNALeu-cox2 region. The male genitalia were compared with the morphological descriptions in the literature, and the gene sequences with the ones deposited at GenBank. Both analyses confirmed the presence of H. armigera in the collecting locations. The pest geographic range was expanded to the South region of the country.

Lepidoptera (Insecta) associated with soybean in Argentina, Brazil, Chile and Uruguay

“…The recent detection of H. armigera (CZEPAK et al, 2013;EMBRAPA 2013;SPECHT et al, 2013), previously not reported in the American continent become another important aspect for agriculture in Brazil. H. armigera is reported to feed in more than 300 native and cultivated host plants (LIU et al, 2004).…”

“…MARQUES & LAMAS, 2006). Anyway, the lack of taxonomists was directly related to the delay in the report of H. armigera in Brazil, which has affected high value crops such as cotton and soybean (CZEPAK et al, 2013;EMBRAPA 2013;SPECHT et al, 2013). This indicates the vulnerability of Brazil and other countries in South America concerning the change of the status of native arthropod species, due to climate change, the invasion risk of quarantine pests, management, and the introduction of new crops or cultivars.…”

“…However, several aspects have affected the appropriate management of this group of pests: 1) changes in systematic positions and nomenclature (LAFONTAINE & SCHMIDT, 2010); 2) changes detected in specific compositions of lepidopteran communities associated with soybean (GUEDES et al, 2010;SOSA-GÓMEZ et al, 2010;MOSCARDI et al, 2012); and 3) recent detection of Helicoverpa armigera (Hübner), which has economy impact in this crop (CZEPAK et al, 2013;EMBRAPA, 2013;SPECHT et al, 2013). This research is a review of reports of lepidoptera whose larvae were collected feeding on the soybean crop in Argentina, Brazil, Chile and Uruguay and also updates the systematic position and nomenclature of this taxonomic group.…”

<b>Age and density of eggs of <i>Helicoverpa armigera</i> influence on <i>Trichogramma</i> pretiosum parasitism

“…Recently, the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), which is a cosmopolite and polyphagous pest, has been introduced in Brazil (Czepak, Albernaz, Vivan, Guimarães, & Carvalhais, 2013;Specht, Sosa-Gómez, Paula-Moraes, & Yano, 2013;Gómez et al, 2016). Since then, it has been causing losses in the production of several crops including cotton, corn, soybean, and tomatoes (Bueno, Yamamoto, Carvalho, & Bueno, 2014;Czepak et al, 2013;Mastrangelo et al, 2014;Pratissoli, Lima, Pirovani, & Lima, 2015;Tay et al, 2013).…”

Parasitoides associados com Helicoverpa armigera em áreas de refúgio de algodão, no Oeste da Bahia, Brasil

“…The larvae were collected manually (n =301) in a random transversal "zigzag" sampling as described by CORRÊA-FERREIRA et al (2014) and were individually maintained in polyethylene cups (80 ml) with artificial diet (GREENE et al 1976) until the emergence of moths or parasitoids. All emerged moths were identified as H. armigera comparing their genitalia (HARDWICK 1965, SPECHT et al 2013. Likewise, the parasitized larvae or pupae were identified as H. armigera comparing the larval and pupal exuvia with reference material of H. armigera and Helicoverpa zea (Boddie, 1850) maintained in the Laboratório de Entomologia (Embrapa Cerrados).…”