Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Abstract

-The objective of this work was to identify growth-promoting bacteria isolated from Agaricus blazei and to evaluate their effect on mushroom mycelial growth and productivity. A total of 56 A. blazei-associated bacterial isolates were obtained from casing soil and identified by 16S rRNA gene sequencing. Bacteria were evaluated as to phosphate-solubilization ability, nitrogen-fixation capability, and secretion of cellulase. Superior isolates were tested for their to effect on A. blazei productivity, micelial growth, and on the contents of the polysaccharide-protein complex and of N, P, K, Ca, and Mg. Bacterial isolates were identified as actinobacteria (60%), firmicutes (20%), and proteobacteria (20%). Among them, ten isolates had phosphate-solubilization ability, eight showed nitrogen-fixation capability, and 12 isolates promoted A. blazei mycelium growth. Bacterial inoculation reduces time till harvest in up to 26 days, increases fresh mushroom yield up to 215%, and increases total polysaccharide-protein complex content twofold when compared to the non-inoculated control. The actinobacteria group is the predominant A. blazei-associated phylum.Index terms: Agaricus blazei, bioprospecting, fresh mushroom yield, polysaccharide-protein complex. Termos para indexação: Agaricus blazei, bioprospecção, produção de cogumelos frescos, complexo polissacarídeo-proteínas. Bactérias cultiváveis promotoras do crescimento do cogumelo e seu impacto sobre a produtividade de Agaricus blazei

Genetic diversity, microbiological study and composition of soil associated with wild Pleurotus ostreatus from different locations in Ondo and Ekiti States, Nigeria

“…The occurrence of isolated bacteria and fungi from P. ostreatus and their associated soil at different locations is shown in Tables 3 and 4. The presence of Pseudomonas putida conformed to the findings of Cho et al [22] and Young et al [23]. In their studies, they revealed the bacterium as one of the mushroom growth-promoting bacteria (MGPB) during the growth of P. ostreatus and Agaricus bisporus.…”

Growing edible mushrooms: a conversation between bacteria and fungi

“…The development of MGP also offers an opportunity to maximize the production of alternative low‐yielding cultivated species under controlled conditions, such as the medicinal mushroom Agaricus subrufescens (Zied et al ., ). Young and colleagues () characterized a number of cultivable bacteria isolated from A. subrufescens ‐associated soil showing phosphate‐solubilization ability, nitrogen‐fixation capability, and the ability to promote mycelium growth. Among them, the application of the actinobacteria MGP Microbacterium humi in casing during cultivation contributed to reduce time till harvest in up to 26 days and an increase of fresh mushroom yield up to 215% was reported.…”

VIT-CMJ2: Endophyte of Agaricus bisporus in Production of Bioactive Compounds

“…They generally show endosymbiosis with the host, resulting in mutual benefit. Fungal endophytes in plant help the roots in nutrients absorption, preventing infection and supports in growth (7,9). Twenty three strains of mushroom growth promoting bacteria have been identified from 14 different mushroom farms (7,8).…”

“…Agaricus blazei have been found to be associated with actinobacteria that help to reduce the harvest time and at the same time increases the total polysaccharide-protein complex content (9). So far, association studies were used to analyze interaction between the microbes and mushroom substrate or mycelium.…”