Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids

Sakabe, Róberson; Moraes, Flávio Ruas de; Belo, Marco Antônio de Andrade; Pilarski, Fabiana; Moraes, Julieta Rodini Engrácia de

doi:10.1590/s0100-204x2013000300010

Cited by 24 publications

(20 citation statements)

References 22 publications

(27 reference statements)

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“…Gradually, the nuclei mobilize to the periphery of the cytoplasm forming Langhans cells (PETRIC et al, 2003a,b;Belo et al, 2005;. Sakabe et al (2013) observed these cells 8 DPI in Nile tilapia supplemented with high levels of linseed oil. In our study, it was no possible observed them.…”

Section: Resultsmentioning

confidence: 99%

Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus

Petrillo¹,

Claudiano²,

Yunis‐Aguinaga³

et al. 2017

Biosci. J.

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ABSTRACT:The aim of this study was to evaluate the effect of parenteral administration of dexamethasone and levamisole on the accumulation of macrophages and formation of giant cells in chronic inflammation by foreign body and blood parameters in pacu (Piaractus mesopotamicus). It was used 50 mg/kg of levamisole and 2.0 mg/kg of dexamethasone and the combination of both drugs. Coverslips were implanted under the skin. After 2, 7, and 15 days postimplantation (DPI), fish were anesthetized for removal of coverslips and the number of macrophages and giant cells were count. It was also collected blood from the caudal vessel to evaluate: red blood cell count, hematocrit, hemoglobin concentration, leukocytes count, thrombocytes count, MCV, and MCHC. We observed that dexamethasone affects negatively the formation of giant cells in the chronic inflammation for foreign body. Levamisole, despite being immunostimulatory in several species, showed limited action. However, it was enough to counteract the effect of dexamethasone; the association of the drugs did not interfere significantly in erythrocyte and leukocyte number in most of the treatments and times studied. In dexamethasone group there was a reduction in the number of erythrocytes and hemoglobin associated with increased mean corpuscular volume, suggesting slight macrocytic anemia. At 15 DPI, most groups showed the recovery of hematologic response. As in mammals, dexamethasone affects negatively the inflammatory response. Levamisole showed little effect by itself. However, in some parameters the association of both drugs causes similar response to control and naïve groups, showing the antagonistic effect of these drugs.

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Section: Resultsmentioning

confidence: 99%

Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus

Petrillo¹,

Claudiano²,

Yunis‐Aguinaga³

et al. 2017

Biosci. J.

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“…However, the development of colonies may result in local irritation, since multifocal granulomatous dermatitis was found in infested fish, characteristic of a chronic pathological process (Ulrich et al, 2007). Mononuclear infiltration and giant cell formation have also been reported in the evolution of chronic inflammatory response in teleost fish (Belo et al, 2005(Belo et al, , 2012Sakabe et al, 2013), including peritrich infestation (Valladão et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Host-parasite relationship during Epistylis sp. (Ciliophora: Epistylididae) infestation in farmed cichlid and pimelodid fish

Pádua¹,

Martins

Valladão

et al. 2016

Pesq. agropec. bras.

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-The objective of this work was to describe the host-Epistylis sp. relationship during infestation on farmed fish. Five Nile tilapia (Oreochromis niloticus) and ten hybrid surubim catfish (Pseudoplatystoma reticulatum x P. corruscans), all diseased, were used for in vivo morphological analysis of sessile peritrichs by contrast microscopy. Fragments of infected tissues were subjected to histological processing and scanning electron microscopy. Epistylis sp. caused hemorrhagic ulcer disease, and cichlids were more prone to develop infestations throughout the body surface due to the attachment of the colonies to the scales, which did not occur with pimelodids. Multifocal granulomatous dermatitis was observed, associated with the hydropic degeneration of the epithelium and to ulcerative areas of necrosis. Microscopic examination showed the presence of bacterial microflora associated to Epistylis sp. peduncles. Therefore, this species can be considered a portal of entry for opportunistic bacteria.Index terms: Oreochromis niloticus, Pseudoplatystoma reticulatum, Pseudoplatystoma corruscans, histopathology, pathogenesis, sessile peritrich. Relação hospedeiro-parasita durante infestação por Epistylis sp. (Ciliophora: Epistylididae) em peixes ciclídeos e pimelodídeos cultivadosResumo -O objetivo deste trabalho foi descrever a relação hospedeiro-Epistylis sp. durante infestação em peixes cultivados. Foram utilizados cinco tilápias-do-nilo (Oreochromis niloticus) e dez híbridos de surubim (Pseudoplatystoma reticulatum x P. corruscans), todos parasitados, para análise morfológica in vivo de peritríquios sésseis por microscopia de contraste de fase. Fragmentos de tecidos infectados foram processados para histologia e microscopia eletrônica de varredura. Epistylis sp. causou doença ulcerativa hemorrágica, e os ciclídeos foram mais susceptíveis ao parasitismo na superfície do corpo em razão da fixação das colônias nas escamas, o que não ocorreu nos pimelodídeos. Foi observada ocorrência de dermatite granulomatosa multifocal, associada à degeneração hidrópica do epitélio e às áreas ulcerativas de necrose. O estudo microscópico mostrou a presença de bactérias associadas aos pedúnculos de Epistylis sp. Portanto, esta espécie pode ser considerada porta de entrada para bactérias oportunistas.

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“…Moreover, the high concentration of glucocorticoids due to handling stress and treatment could favor energy metabolism by stimulating gluconeogenesis, increasing the bioavailability of free fatty acids resulting from the breakdown of triglycerides [22,23]. Sakabe et al [24], Belo et al [25,26] and Castro et al [27] reported the occurrence of stress in animals, resulting of handling and treatment. According to these authors, the organic response to stress conditions is complex, involving neuro-endocrine mechanisms with the release of glucocorticoids and catecholamines which act by increasing the metabolic activity and consequently altering the organic balance.…”

Section: Discussionmentioning

confidence: 99%

Clinical safety of amino acids and vitamins administered through parenteral route

Moraes¹,

Scherma²,

Prado³

et al. 2017

Int J Vet Sci Res

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The parenteral use of amino acids and vitamins has been used to improve performance in animal production. However, few studies demonstrating the toxicity of these compounds. The present investigation evaluated the toxicological and clinical safety of the injectable supplement compound by amino acids and vitamins, administered by intramuscular route in Wistar rats. 56 females (± 220g), pubescent virgins, were randomly divided into seven groups (n=8): Treated with dose of 0.2, 2 and 4 mL, sampled 24 and 96 hours post-treatment (HPT), totaling six groups and one control group (Physiology Standard). Signifi cant increase in the enzymatic activity of serum ALT, AST, LDH and ALP was observed in animals treated with 2 and 4 mL (24 HPT). However, these changes returned to baseline levels 96 HPT, except in serum AST and ALP in animals treated with 4 mL. CK-NAC showed dose-response effect, since the higher compound dose was followed by proportional increase in the circulating levels of this enzyme, associated to muscle degeneration and necrosis area in rats treat with 4mL at 96 HPT. The evaluation of hepatic function did not result in signifi cant changes in the serum levels of triglycerides, cholesterol, albumin, urea and creatinine in the comparison between rats treated with 0.2 mL and control rats, demonstrating the innocuousness of this treatment on the activity of liver and kidney. However, deleterious effects were observed in animals subjected to doses of 2 and 4 mL, including observed increased splenic and hepatosomatic response, signifi cant decrease in circulating albumin levels and signifi cant leukocytosis, which is characterized by neutrophilia, followed by lymphopenia demonstrating the compound irritating effect on muscle tissue.

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Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids

Cited by 24 publications

References 22 publications

Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus

Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus

Host-parasite relationship during Epistylis sp. (Ciliophora: Epistylididae) infestation in farmed cichlid and pimelodid fish

Clinical safety of amino acids and vitamins administered through parenteral route

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