Storage protein profile and amino acid content in wild rice Oryza glumaepatula

Abstract

-The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan) in comparison to the two analized rice cultivars. The albumin and glutelin profiles in SDS-Page were distinct between rice cultivars and O. glumaepatula. This wild species has the potential to increase the nutritional quality of rice storage protein through interspecific crosses.Index terms: Oryza sativa, genetic resources, nutritional quality, plant breeding, SDS-Page. Perfil da proteína de reserva e conteúdo de aminoácidos no arroz silvestre Oryza glumaepatulaResumo -O objetivo deste trabalho foi determinar os perfis de proteína total e o conteúdo das quatro principais frações proteicas (albumina, globulina, prolamina e glutelina) e de aminoácidos no endosperma da espécie de arroz silvestre Oryza glumaepatula. O experimento foi realizado com 29 acessos dessa espécie, coletados em 13 locais no Brasil, e duas cultivares comerciais. Amostras de proteínas foram preparadas com grãos secos, polidos e moídos, para obtenção de uma farinha seca e homogênea, usada no preparo dos estratos. Acessos de O. glumaepatula foram identificados com os maiores níveis de proteína total, frações proteicas de albumina e glutelina, e aminoácidos (com exceção do triptofano), em comparação às duas cultivares de arroz avaliadas. Os perfis de albumina e glutelina em SDS-PAGE foram distintos entre as cultivares de arroz e O. glumaepatula. Essa espécie silvestre tem o potencial de aumentar a qualidade nutricional da proteína de reserva do arroz por meio de cruzamentos interespecíficos.Termos para indexação: Oryza sativa, recursos genéticos, qualidade nutricional, melhoramento de plantas, SDS-Page.

Food Composition Data: Edible Plants in Pantanal

“…(Filgueiras et al 2015;Flora do Brasil 2020). Among these, O. glumaepatula presents high levels of total fractions of protein, albumin, and glutelin (protein, albumin, and glutelin fractions) (Santos et al 2013).…”

Gluten Immunogenic Peptides as Standard for the Evaluation of Potential Harmful Prolamin Content in Food and Human Specimen

“…β Differences among varieties. (a) Osborne method [102] (b) Kjeldahl method [101] (c) SDS-Page [103]. ND, Not determined.…”

Substitution mapping of the major quantitative trait loci controlling stigma exsertion rate from Oryza glumaepatula

“…Several hybrid pollen sterility genes between O. sativa and O. glumaepatula have been identi ed, such as S12, S22, S23, S27 and S28 (Sano 1994;Sobrizal et al 2000a, b;Yoshimura 2001, 2002;Sakata et al 2014;Fang et al 2019). It was found that the endosperm of O. glumaepatula had the high levels of total protein, albumin, and glutelin protein fractions and amino acids, showing the potential to increase the nutritional quality of rice storage protein (Santos et al 2013). O. glumaepatula also showed the rapid internodal elongation potential under partial submergence (Sasayama et al 2018), as well as its important yield and yield component traits (Zhang et al 2015;Bhatia et al 2017).…”