Diversidade genética de acessos do banco de germoplasma de mangaba em Sergipe

Abstract

Genetic diversity of accessions of the mangaba germplasm bank in Sergipe, BrazilAbstract -The objective of this work was to estimate the genetic variability of mangaba accessions of natural populations, from 11 locations, using RAPD markers. The accessions belong to Banco Ativo de Mangaba of Embrapa Tabuleiros Costeiros, in Itaporanga d'Ajuda, SE, Brazil. A total of 13 primers were used, which generated 82 fragments, of which 78 (95%) were polymorphic. Genetic analysis among regions showed low genetic diversity; however, genetic similarity ranged from 0.02 to 0.91, for the 55 accessions. Divergent groups were identified by UPGMA and ACoP clustering. The least similar accessions were derived from Ipiranguinha (Conde, PB, Brazil) and Preguiça (Indiaroba, SE, Brazil), and the most similar from Jandaíra (Costa Azul, BA, Brazil). From the total, 49 accessions were genetically distinct and six were similar. By using RAPD markers, it was possible to obtain a unique molecular profile, besides estimating the variability among the accessions evaluated. The Banco Ativo de Germoplasma de Mangaba of Embrapa Tabuleiros Costeiros shows low genetic diversity among locations.

Genetic diversity of the Neotropical tree Hancornia speciosa Gomes in natural populations in Northeastern Brazil

“…The absolute values were 0.207 (Region 1), 0.197 (Region 2), and 0.229 (Region 3). These results are close to those obtained in studies with dominant markers in natural populations; for example, 0.163-0.236 for Eugenia dysenterica (Zucchi, 2002), 0.25-0.29 to Polylepis australis (Julio et al, 2008), 0.11-0.26 and 0.25-o 0.42 for Hancornia speciosa (Costa et al, 2011;Martins et al, 2012), 0.30-0.42 for Acrocomia aculeata (Oliveira et al, 2012), and 0.24-0.27 for Metrodorea nigra (Moraes Filho et al, 2015). In addition, these results are much higher than those obtained for autogamous species such as Theobroma speciosum (Ĥ E = 0.051-0.095; Giustina et al, 2014), and Coffea arabica (Ĥ E = 0.05-0.15; Aga et al, 2005).…”

Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers

“…Indeed, our results showed this low locus variability, with four monomorphic markers and five markers with two or three alleles per locus. The low H E value (0.2) obtained in our analysis is similar to that reported in a previous study (Costa et al, 2011) and suggested low genetic diversity in mangabeira populations. The polymorphic loci are important indicators of the level of genetic variation in a given area.…”

“…To date, there have been very few studies of genetic diversity in mangabeira populations, and all of them use RAPD markers as the molecular tool (Moura et al, 2005(Moura et al, , 2011Costa et al, 2011;Silva et al, 2011) due to the absence of the species genome information. As noted, only six sequences of H. speciosa appear in the GenBank database.…”

Genetic variability in accessions of the acerola germplasm bank of Universidade Federal Rural de Pernambuco, Brazil

“…The H E values in the present study were similar to those in studies with natural populations, e.g., 0.27 to 0.33 for T. pallida (Zimback et al, 2004), 0.25 to 0.29 for Polylepis australis (Julio et al, 2008), 0.11 to 0.26 for Hancornia speciosa (Costa et al, 2011) and 0.25 to 0.42 for Acrocomia aculeata (Oliveira et al, 2012), suggesting a strong genetic basis.…”