Phytogeographical origin of Madeiran common beans based on phaseolin patterns

Abstract

-The objective of this work was to determine the geographic origin of the Madeiran common bean (Phaseolus vulgaris) gene pool. Phaseolin patterns of 50 accessions representing the diversity of common bean collected in Madeira, Portugal, and conserved in the ISOPlexis Germplasm Bank, were analysed using the Experion automated electrophoresis system, based on lab-on-a-chip technology. Five common bean standard varieties with typical phaseolin patterns were used to determine the phytogeographical origin of the Madeiran common bean accessions. Ninety two percent of the accessions exhibited a phaseolin pattern consistent with the one of common bean types belonging to the Andean gene pool, while the origin of the remaining 8% of the accessions was indistinguishable. The application of a similarity coefficient of 85%, based on Pearson correlations, increases the number of accessions with uncertain pattern. The analytical approach used permitted the determination of the origin of the common bean gene pool, which is Andean in 98% of the cases, and clustering of the observed variability among the Madeiran common beans.

Morphological evaluation of common bean diversity on the Island of Madeira

“…In our study, it is notorious the dominance of bean accessions with large seeds, indicating their possible Andean origin. This hypothesis is supported by the data of phaseolin pattern electrophoresis analysis of the Madeiran beans (da Silva et al 2010). Our data are consistent with the results of the analysis of 88 germplasm accessions from northern Portugal performed by Rodiño et al (2001b), who also observed the predominance of the Andean germplasm (seed size) over germplasm of Mesoamerican origin.…”

Nutritional and Mineral Variability in 52 Accessions of Common Bean Varieties (<i>Phaseolus vulgaris</i> L.) from Madeira Island

“…Fifty-two bean accessions from the ISOPlexis Genebank were selected based on their representativeness of crop As-Ash; N-Nitrogen; P-Phosphorous; K-Potassium; Mg-Magnesium; Fe-Iron; Cu-Copper; Zn-Zinc; B-Boron. 1 Contender MBG CODE: PHA-0947, 2 Sanilac MBG CODE: PHA-0948, 3 Tendergreen MBG CODE: PHA-0949, 4 Pampa MBG CODE: PHA-0950, 5 Boyaca MBG CODE: PHA-0951 are International Bean Standards from Galicia, Spain, 6,7 Commercial bean varieties from a local supermarket. * Lowest value registered.…”

“…At the same time, its nutritional composition is conditioned by factors such as genotype, origin, environmental and growing conditions, influencing the quality of the bean seeds [5]. Historical records [6] corroborated by the analysis of phytogeographic origin [7] indicate that common bean has been introduced into Madeira in the beginning of sixteenth century, probably from Brazil, South America, represented almost exclusively by the Andean gene pool, C (Contender) and T (Tendergreen) genotypes. For centuries the farmers maintained under cultivation a wide diversity of common bean landraces, both with indeterminate and determinate growth, divided into green or seed bean.…”

Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chip electrophoresis

“…It offers a platform to quickly identify grain varieties and estimate protein sizes. Using the microfluidic LoaC the geographic origin of the Madeiran common bean gene pool was reported by Da Silva et al (2010). The aim of this study was to analyze the seed storage proteins of the Serbian grain legumes cultivars.…”