Transgene inheritances and genetic similarities of near isogenic lines of genetically modified common beans

Pinheiro, Patrícia Valle; Faria, J. C.; Nogueira, Elsa Oliveira Paranaguá e Lago; Aragão, F. J. L.

doi:10.1590/s0100-204x2009000900015

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…29 In the present study, differences in environmental conditions throughout the three years seemed to have greater impact on insect abundance and diversity than the GM line itself. The total number of specimens rose from the first year to the third year, probably due to agrochemicals applied on the experimental field for management purposes in the first year and at a lower rate in the second and third year ( Table 6).…”

Section: Discussionmentioning

confidence: 45%

Populational survey of arthropods on transgenic common bean expressing therepgene fromBean golden mosaic virus

Pinheiro¹,

Quintela²,

Junqueira³

et al. 2014

GM Crops & Food

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Section: Discussionmentioning

confidence: 45%

Populational survey of arthropods on transgenic common bean expressing therepgene fromBean golden mosaic virus

Pinheiro¹,

Quintela²,

Junqueira³

et al. 2014

GM Crops & Food

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“…Unfortunatelly, RAPD are dominant markers, meaning they could not differentiate homozygous A/A from heterozygous A/a which give limitations for fingerprinting and mapping. However, they have been used as markers of important traits to be introgressed in near isogenic lines (Pinheiro et al, 2009).…”

Section: Randomly Amplified Polymorphic Dna (Rapd)mentioning

confidence: 99%

Molecular markers and their application for DNA fingerprinting and genetic diversity studies in Coffea species Marka molekuler dan penerapannya untuk studi sidik jari DNA dan keragaman genetik pada spesies Coffea

Priyono¹,

Putranto²

2016

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AbstrakStrategi klasik yang meliputi perbandingan anatomi, fisiologi dan sitogenetika telah banyak diterapkan untuk mengidentifikasi karakter tertentu serta untuk menentukan keragaman dan hubungan antar dan intra spesies. Namun, saat ini penanda molekuler telah melengkapi strategi sebelumnya dengan sangat cepat. Berbagai jenis penanda molekuler digunakan untuk menilai tingkat polimorfisme DNA. Penanda molekuler ini diklasifikasikan sebagai penanda berbasis hibridisasi dan berbasis Polymerase Chain Reaction (PCR). Dalam beberapa tahun terakhir, sistem penanda DNA yang berbeda seperti Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) yang juga disebut Mikrosatelit, Single Nucleotide Polymorphims (SNPs) dan lain-lain telah dikembangkan dan diterapkan pada berbagai spesies tanaman. Penanda molekuler ini dapat digunakan untuk sidik jari DNA dan studi keragaman genetik. Sidik jari berdasarkan DNA telah banyak digunakan dalam ilmu forensik, juga memiliki berbagai aplikasi dalam pemuliaan tanaman. Tulisan ini memberikan overview tentang berbagai penanda molekuler dan aplikasinya untuk sidik jari dan kajian keragaman genetik tanaman berdasarkan DNA pada berbagai spesies tanaman, dan secara khusus pada Coffea sp.AbstractConventional strategies including comparative anatomy, physiology and cytogenetics were applied to identify the certain character as well as to determine inter- and intra-species diversity and relationships. However, more recently molecular markers have very rapidly complemented the previous strategies. Various types of molecular markers are used to assess DNA polymorphism. They are classified as hybridization-based markers and polymerase chain reaction (PCR) based markers. In recent years, different DNA marker systems such as Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) which also called as microsatellites, Single Nucleotide Polymorphims (SNPs) and others have been developed and applied to a range of plant species. These molecular markers can be used for DNA fingerprinting and genetic diversity study. DNA fingerprinting has been widely used in forensic science, but is has also a variety of application in plant breeding. This paper provides an overview about various molecular markers and their application for DNA plant fingerprinting and genetic diversity, especially in Coffea sp.

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“…, Pinheiro et al. ). However, atypical non‐Mendelian segregations of transgenes have also been reported in 10 to 50% of independent transgenic lines (Aragão et al.…”

mentioning

confidence: 99%

RNAi‐based Bean golden mosaic virus‐resistant common bean (Embrapa 5.1) shows simple inheritance for both transgene and disease resistance

et al. 2014

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Bean golden mosaic virus (BGMV) is the causal agent of bean golden mosaic of common beans. A transgenic bean line that has been developed based on RNA interference to silence the BGMV rep gene showed immunity to the virus. Crosses were done between the transgenic line and six bean cultivars followed by four backcrosses to the commercial cultivars 'P erola' and 'BRS Pontal'. The transgene locus was consistently inherited from the crosses analysed in a Mendelian fashion in the segregating populations. The disease resistance reaction co-inherited with the transgene. Nevertheless, the expression of disease resistance displayed a dosage effect phenomenon in the F 1 generation. The analysis of the homozygous near-isogenic lines in field conditions, under high BGMV disease incidence, indicated that the transgenic lines were completely resistant. These results show the strength of the disease resistance obtained, the stability of the trait across generations and its usefulness in the management of a disease for which there is no reported Phaseolus germplasm with immunity.

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Transgene inheritances and genetic similarities of near isogenic lines of genetically modified common beans

Cited by 3 publications

References 19 publications

Populational survey of arthropods on transgenic common bean expressing therepgene fromBean golden mosaic virus

Populational survey of arthropods on transgenic common bean expressing therepgene fromBean golden mosaic virus

Molecular markers and their application for DNA fingerprinting and genetic diversity studies in Coffea species Marka molekuler dan penerapannya untuk studi sidik jari DNA dan keragaman genetik pada spesies Coffea

RNAi‐based Bean golden mosaic virus‐resistant common bean (Embrapa 5.1) shows simple inheritance for both transgene and disease resistance

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