RNAi‐based Bean golden mosaic virus‐resistant common bean (Embrapa 5.1) shows simple inheritance for both transgene and disease resistance

Abstract

Bean golden mosaic virus (BGMV) is the causal agent of bean golden mosaic of common beans. A transgenic bean line that has been developed based on RNA interference to silence the BGMV rep gene showed immunity to the virus. Crosses were done between the transgenic line and six bean cultivars followed by four backcrosses to the commercial cultivars 'P erola' and 'BRS Pontal'. The transgene locus was consistently inherited from the crosses analysed in a Mendelian fashion in the segregating populations. The disease resistance reaction co-inherited with the transgene. Nevertheless, the expression of disease resistance displayed a dosage effect phenomenon in the F 1 generation. The analysis of the homozygous near-isogenic lines in field conditions, under high BGMV disease incidence, indicated that the transgenic lines were completely resistant. These results show the strength of the disease resistance obtained, the stability of the trait across generations and its usefulness in the management of a disease for which there is no reported Phaseolus germplasm with immunity.

Agronomic Performance and Yield Stability of the RNA Interference‐Based Bean golden mosaic virus‐Resistant Common Bean

“…In all backcrossing cycles and generations, the presence of the transgene was checked through polymerase chain reaction with specific primers (Bonfim et al, 2007). F 1 plants of all the backcrossing cycles were also inoculated with the BGMV and evaluated for BGMV reaction (Faria et al, 2014). Thus the segregation pattern of the presence of the transgene and of resistance to BGVM was always checked during the backcrossing phase.…”

Population Dynamics of Whiteflies and Associated Viruses in South America: Research Progress and Perspectives

“…Among them, tomato plants have been explored, in which studies conducted with different genotypes concluded that the presence of high levels of allelochemicals such as acyl sugars (AS) and zingiberene (ZGB) associated with the presence of the Mi gene might be related to greater resistance to whitefly [214]. Besides the searching of resistant genotypes for whiteflies, studies are conducted to generate resistant cultivars to diseases, and the BGMV resistant bean is an example of it, in which Brazilian researchers developed a transgenic common bean resistant to this virus [215]. The use of repellent plants can also reduce the number of insecticide spraying, or even in organic crops.…”

Small RNA Based Genetic Engineering for Plant Viral Resistance: Application in Crop Protection

“…Stable transgenic plants for a variety of crops were generated expressing small RNAs in different ways and their reactions to targeted viruses were tested in both laboratory and field condition ( Supplementary Table S1 ). In some studies, the durability of resistance was tested for many generations (Wang et al, 2001, 2016; Liu et al, 2007; Cruz et al, 2014; Faria et al, 2014). According to the International Service for the Acquisition of Agri-Biotech Applications (ISAAA) website, dozens of transgenic crops resistance to virus generated with SRGE were approved for commercial release ( Supplementary Table S2 ).…”

“…Multiple-viruses resistant soybean was also generated by expressing multiple short hairpin targeting Rep of AMV, BPMV, and SMV (Zhang et al, 2011b). BGMV-partial-resistant common bean was initially generated with S-PTGS mechanism targeting CP and completely resistant transgenic line was recently obtained using hp-PTGS targeting AC1 gene (Faria et al, 2006, 2014; Aragao et al, 2013). BGMV-resistant common bean was approved for commercial release in Brazil ( Supplementary Table S2 ) while no commercial release of SRGE based viral resistant peanut and soybean were reported.…”