Esterilização química de meio de cultura no cultivo in vitro de antúrio

Cardoso, Jean Carlos

doi:10.1590/s0100-204x2009000700020

Cited by 15 publications

(12 citation statements)

References 7 publications

(10 reference statements)

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“…Chemical sterilization with sodium hypochlorite (NaOCl), proposed initially by Teixeira et al (2005a, b), provided satisfactory results for pineapple (Ananas comosus L. cv Smooth Cayenne) (Texeira et al, 2006), sequoia (Sequoia sempervirens L.) (Ribeiro et al, 2011), and eucalyptus (Eucalyptus pellita L. and Eucalyptus benthamii Maiden et Cambage) (Teixeira et al, 2008;Brondani et al, 2013). Recently another chemical compound, chlorine dioxide (ClO 2 ), a stabilized gas, used by Cardoso (2009) in the sterilization of culture media for anthurium (Anthurium andraeanum Lind.) and gerbera, eliminated contamination without causing phytotoxicity to plants (Cardoso, 2009;Cardoso and Silva, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Pais¹,

Silva²,

Souza³

et al. 2016

Afr. J. Biotechnol.

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Micropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for sterilization of culture media and glassware used during rooting of micropropagated Gerbera hybrida cv. Essandre. Two experiments were carried out. In the first one, treatments consisted of control I (no sterilization), control II (thermal sterilization), and total active chlorine concentrations of 0.0005, 0.001, 0.002 and 0.003%. In the second experiment, based on the results observed in the first experiment, treatments consisted of control I (thermal sterilization) and II (chemical sterilization), and total active chlorine concentrations of 0.002, 0.0025 and 0.003%. Plant behavior was assessed based on the length of aerial part and roots, number of roots, and dry biomass of plants. Results showed that the addition of an active chlorine concentration of 0.003% to culture media provided total control of contaminants, and there were no significant differences regarding the variables analyzed between plants obtained with thermal sterilization and with sodium hypochlorite sterilization. Thus, chemical sterilization can be used as a replacement for thermal sterilization of nutrition media for rooting of gerbera in vitro.

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Section: Introductionmentioning

confidence: 99%

“…Recently another chemical compound, chlorine dioxide (ClO 2 ), a stabilized gas, used by Cardoso (2009) in the sterilization of culture media for anthurium (Anthurium andraeanum Lind.) and gerbera, eliminated contamination without causing phytotoxicity to plants (Cardoso, 2009;Cardoso and Silva, 2012).…”

Section: Introductionmentioning

confidence: 99%

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Pais¹,

Silva²,

Souza³

et al. 2016

Afr. J. Biotechnol.

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show abstract

“…Currently, ultraviolet light and chlorine-based products, such as the stabilized liquid chlorine dioxide (ClO 2 ), are very efficient, the latter even when used at very low concentrations (Cardoso, 2009;Cardoso & Teixeira da Silva, 2012). ClO 2 is also considered an excellent disinfectant, with bactericidal and fungicide action.…”

Section: Some Necessary Precautionsmentioning

confidence: 99%

Reuse water and urban horticulture: alliance towards more sustainable cities

Bizari

Cardoso

2016

Hortic. Bras.

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The need to rethink current models of using water resources in the various sectors of human activity is escalating, as thousands of people in different regions of the world are suffering from clean water shortage for their basic daily needs. In this context, the use of recycled water from treated domestic sewage in agricultural activities is gaining ground. Reuse water can combine environmental protection and high agricultural productivity, especially for simultaneously carrying plant nutrients and minimizing demands for catching "noble" fresh water from streams and water sources for irrigation, currently the highest water consuming activity. The increasing urbanization contributes to generate, but also to foster the treatment and use of wastewater, since urban areas are, at one time, chief water consumers and chief sewage producers. Although there are cultural and sanitary impediments for using recycled water on large scale and the knowledge about possible existing treatments is still limited, a safe alternative to integrate the reuse water into the productive process is its use in irrigation projects involving non edible species. Among these, plants used in floriculture and landscaping can be great allies for the development of wastewater-based urban and peri-urban horticulture, encouraging local development, creating jobs and aiding to environmental protection. We critically discuss these possibilities in this article.

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“…Large energy consumption is also a drawback because the heating sterilization usually requires heating the entire sample volume. Thus, some nonheating sterilization methods, such as UV irradiation [2], ion beam irradiation [3], electrical [4] and chemical [5] methods, have been proposed to avoid these problems. Among them, irradiation methods require expensive instruments, and are not to be easily popularized.…”

Section: Introductionmentioning

confidence: 99%

Low‐Voltage Pulsed Electric Field Sterilization on a Microfluidic Chip

et al. 2013

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A polyimide substrate based microfluidic chip with thousands of comb-shaped microelectrodes has been designed, fabricated, and tested for sterilization of bacteria by using pulsed electric field. The performance of bacteria sterilization as functions of the electric field strength, pulse number and width, treatment buffer, bacteria growth status, and bacteria enrichment by positive dielectrophoresis has been experimentally investigated on the microfluidic chip. Experimental results show that only 100 V are sufficient to obtain good sterilization of Escherichia coli. Higher electric field strength, bacteria enrichment by positive dielectrophoresis, longer pulse time, buffer with fewer components and nutritions, and suitable bacteria growth status also improve the sterilization of bacteria. In addition, configuration of the microelectrode array affects bacteria sterilization. This microfluidic device allows one to preconcentrate bacteria to a region with high electric field strength by using positive dielectrophoresis, and subsequently kill the enriched bacteria by applying a pulsed electric field through the same microelectrode array.

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Esterilização química de meio de cultura no cultivo in vitro de antúrio

Cited by 15 publications

References 7 publications

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Reuse water and urban horticulture: alliance towards more sustainable cities

Low‐Voltage Pulsed Electric Field Sterilization on a Microfluidic Chip

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